Reconstruction of Functional Connectivity from Multielectrode Recordings and Calcium Imaging

Bonifazi, P.; Massobrio, Paolo

doi:10.1007/978-3-030-11135-9_9

Cited by 6 publications

(5 citation statements)

References 44 publications

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“…Functional inferences about circuit connections are based on the timing of neuronal activities between groups of neurons. Last, effective connectivity refers to the ability of neurons to influence other neuronal populations that they are not necessarily directly connected to ( 62 – 64 ). We speculate that in the highly innervated HPV − HNSCC slices, effective connectivity results in the high evoked activity measured.…”

Section: Discussionmentioning

confidence: 99%

Functional neuronal circuits promote disease progression in cancer

Restaino

Walz

Vermeer³

et al. 2023

Sci. Adv.

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The molecular and functional contributions of intratumoral nerves to disease remain largely unknown. We localized synaptic markers within tumors suggesting that these nerves form functional connections. Consistent with this, electrophysiological analysis shows that malignancies harbor significantly higher electrical activity than benign disease or normal tissues. We also demonstrate pharmacologic silencing of tumoral electrical activity. Tumors implanted in transgenic animals lacking nociceptor neurons show reduced electrical activity. These data suggest that intratumoral nerves remain functional at the tumor bed. Immunohistochemical staining demonstrates the presence of the neuropeptide, Substance P (SP), within the tumor space. We show that tumor cells express the SP receptor, NK1R, and that ligand/receptor engagement promotes cellular proliferation and migration. Our findings identify a mechanism whereby intratumoral nerves promote cancer progression.

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Section: Discussionmentioning

confidence: 99%

Functional neuronal circuits promote disease progression in cancer

Restaino

Walz

Vermeer³

et al. 2023

Sci. Adv.

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“…Alternative methods that rely on calcium imaging to evaluate neuronal network activity in COs have been recently developed (Bershteyn et al, 2017;Lancaster et al, 2017;Mansour et al, 2018;Sakaguchi et al, 2019;Watanabe et al, 2017) and would greatly complement the analyses we report here. These methods permit the quantitation of changes in intracellular calcium levels in neuronal and glial cultures (Bonifazi and Massobrio, 2019;Wong, 1998) and have been adapted for use in dissociated organoid cultures by Sakaguchi et al The primary advantage of calcium-imaging-based methods is their ability to measure functional activity with spatial resolution. A combined approach that incorporates both calcium imaging and MEA techniques would therefore provide the advantage of analyzing functional activities with both high temporal and spatial resolution and allow a more accurate assessment of neural network dynamics in COs.…”

Section: Discussionmentioning

confidence: 99%

Electrophysiological Maturation of Cerebral Organoids Correlates with Dynamic Morphological and Cellular Development

et al. 2020

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Summary Cerebral organoids (COs) are rapidly accelerating the rate of translational neuroscience based on their potential to model complex features of the developing human brain. Several studies have examined the electrophysiological and neural network features of COs; however, no study has comprehensively investigated the developmental trajectory of electrophysiological properties in whole-brain COs and correlated these properties with developmentally linked morphological and cellular features. Here, we profiled the neuroelectrical activities of COs over the span of 5 months with a multi-electrode array platform and observed the emergence and maturation of several electrophysiologic properties, including rapid firing rates and network bursting events. To complement these analyses, we characterized the complex molecular and cellular development that gives rise to these mature neuroelectrical properties with immunohistochemical and single-cell transcriptomic analyses. This integrated approach highlights the value of COs as an emerging model system of human brain development and neurological disease.

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“…Compared with other analyses, ALFF analysis can suppress non-specific signals more effectively, in order to significantly improving the neuron specificity for detecting spontaneous activity in brain regions ( 75 ). The FC analysis focuses on the similarity of spontaneous brain activity within and between regions, with the ALFF activation area as the point of interest ( 76 ). Changes in the ALFF and FC values of DCG, SFG, and bilateral OG suggest that patients with CFS have increased hemodynamic response to local neural activity or the brain's compensatory response to fatigue.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Prolong Life With Nine Turn Method (Yan Nian Jiu Zhuan) Qigong on Brain Functional Changes in Patients With Chronic Fatigue Syndrome in Terms of Fatigue and Quality of Life

Xie

Guan

et al. 2022

Front. Neurol.

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BackgroundChronic fatigue syndrome (CFS) is characterized by persistent fatigue, which often leads to physical and psychological damage. The Prolong Life with Nine Turn method (PLWNT) Qigong is considered as one of the complementary treatments for improving symptoms in patients with CFS. In this study, we used functional magnetic resonance imaging (fMRI) to explore the effects of PLWNT intervention on the subjects with CFS.MethodsThirty four CFS patients were randomly divided into PLWNT group and cognitive behavioral therapy (CBT) group. Both groups were taught by a highly qualified professor at the Shanghai University of Traditional Chinese Medicine once a week and were supervised online during the remaining 6 days at home, over 12 consecutive weeks. We calculated the regional rs-fMRI index amplitude of low-frequency fluctuations (ALFF) for all subjects. To study the changes of the brain network, we used the brain regions with significant differences in ALFF as the regions of interest for whole-brain functional connectivity (FC) analysis. The Multi-dimensional Fatigue Inventory 20 (MFI-20) and Short Form 36-item Health Survey (SF-36) were used for clinical symptom assessment to explore the possible correlation between the rs-fMRI indicators and clinical variations.ResultsThe ALFF values of the right superior frontal gyrus (SFG), and left median cingulate gyrus (DCG) were increased, whereas those of the left middle occipital gyrus (OG), right middle OG and left middle temporal gyrus (MTG) were decreased in PLWNT group. The FC values between the DCG and middle temporal gyrus (MTG), and those between the left OG and the right OG were enhanced. In addition, the SF-36 were positively with the left OG (r = 0.524), SFG (r = 0.517), and DCG (r = 0.533), MFI-20 were negatively with the SFG (r = −0.542) and DCG (r = −0.578). These results were all corrected by FWE (voxel level p < 0.001, cluster level p < 0.05).ConclusionCFS patients have abnormal regional spontaneous neuronal activity and abnormal functional connections between regions after PLWNT intervention. PLWNT can relieve the fatigue symptoms of CFS patients and improve their quality of life. The study was registered in the American Clinical Trial Registry (12/04/2018). Registration Number is NCT03496961.

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Reconstruction of Functional Connectivity from Multielectrode Recordings and Calcium Imaging

Cited by 6 publications

References 44 publications

Functional neuronal circuits promote disease progression in cancer

Functional neuronal circuits promote disease progression in cancer

Electrophysiological Maturation of Cerebral Organoids Correlates with Dynamic Morphological and Cellular Development

Effects of the Prolong Life With Nine Turn Method (Yan Nian Jiu Zhuan) Qigong on Brain Functional Changes in Patients With Chronic Fatigue Syndrome in Terms of Fatigue and Quality of Life

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