Reconstruction of in vivo-like in vitro model: Enabling technologies of microfluidic systems for dynamic biochemical/mechanical stimuli

Park, Sang Min; Eom, Seongsu; Hong, Hyunsuk; Yoon, Jaeseung; Lee, Seong Jin; Kim, Bum Chang; Kim, Hyung Woo; Kim, Dong Sung

doi:10.1016/j.mee.2018.10.010

Cited by 23 publications

(15 citation statements)

References 149 publications

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“…Tissue engineering has been reported to be useful for regenerative medicine 1,2 and organ replacement therapy for the cutaneous, 3,4 neural, 5,6 ophthalmic, 7 cardiovascular, 8–10 pulmonary, 11,12 and skeletal (bone and cartilage) systems, 13–15 and also for testing the efficacies of new drugs in in vitro cell culture platforms. 16–20 For applications in these fields, scaffolds of biomimetic microstructures have been developed that can reproduce specific functions of the native organ by mimicking in vivo cellular microenvironments. One of the challenges in tissue engineering is to reproduce blood vessels as they exist in most organs.…”

Section: Introductionmentioning

confidence: 99%

Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation

Park

Lee

et al. 2019

J Tissue Eng

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The endothelialization on the poly (ε-caprolactone) nanofiber has been limited due to its low hydrophilicity. The aim of this study was to immobilize collagen on an ultra-thin poly (ε-caprolactone) nanofiber membrane without altering the nanofiber structure and maintaining the endothelial cell homeostasis on it. We immobilized collagen on the poly (ε-caprolactone) nanofiber using hydrolysis by NaOH treatment and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/sulfo- N-hydroxysulfosuccinimide reaction as a cost-effective and stable approach. NaOH was first applied to render the poly (ε-caprolactone) nanofiber hydrophilic. Subsequently, collagen was immobilized on the surface of the poly (ε-caprolactone) nanofibers using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/sulfo- N-hydroxysulfosuccinimide. Scanning electron microscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and fluorescence microscopy were used to verify stable collagen immobilization on the surface of the poly (ε-caprolactone) nanofibers and the maintenance of the original structure of poly (ε-caprolactone) nanofibers. Furthermore, human endothelial cells were cultured on the collagen-immobilized poly (ε-caprolactone) nanofiber membrane and expressed tight junction proteins with the increase in transendothelial electrical resistance, which demonstrated the maintenance of the endothelial cell homeostasis on the collagen-immobilized-poly (ε-caprolactone) nanofiber membrane. Thus, we expected that this process would be promising for maintaining cell homeostasis on the ultra-thin poly (ε-caprolactone) nanofiber scaffolds.

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Section: Introductionmentioning

confidence: 99%

Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation

Park

Lee

et al. 2019

J Tissue Eng

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“…In vivo , cellular responses are influenced by various spatiotemporal biochemical signals (Caddeo et al, 2017 ; Park et al, 2019 ). Within tissues, concentration gradients for soluble components, nutrients, metabolites as well as oxygen and pH exist and are essential for exerting pressures that can stimulate or inhibit basic cellular processes (Przyborski, 2017 ).…”

Section: Building Blocks For Developing Human Tissue Equivalentsmentioning

confidence: 99%

Advances in Engineering Human Tissue Models

Moysidou

Barberio

Owens

2021

Front. Bioeng. Biotechnol.

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Research in cell biology greatly relies on cell-based in vitro assays and models that facilitate the investigation and understanding of specific biological events and processes under different conditions. The quality of such experimental models and particularly the level at which they represent cell behavior in the native tissue, is of critical importance for our understanding of cell interactions within tissues and organs. Conventionally, in vitro models are based on experimental manipulation of mammalian cells, grown as monolayers on flat, two-dimensional (2D) substrates. Despite the amazing progress and discoveries achieved with flat biology models, our ability to translate biological insights has been limited, since the 2D environment does not reflect the physiological behavior of cells in real tissues. Advances in 3D cell biology and engineering have led to the development of a new generation of cell culture formats that can better recapitulate the in vivo microenvironment, allowing us to examine cells and their interactions in a more biomimetic context. Modern biomedical research has at its disposal novel technological approaches that promote development of more sophisticated and robust tissue engineering in vitro models, including scaffold- or hydrogel-based formats, organotypic cultures, and organs-on-chips. Even though such systems are necessarily simplified to capture a particular range of physiology, their ability to model specific processes of human biology is greatly valued for their potential to close the gap between conventional animal studies and human (patho-) physiology. Here, we review recent advances in 3D biomimetic cultures, focusing on the technological bricks available to develop more physiologically relevant in vitro models of human tissues. By highlighting applications and examples of several physiological and disease models, we identify the limitations and challenges which the field needs to address in order to more effectively incorporate synthetic biomimetic culture platforms into biomedical research.

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“…[1] Due to their unique properties such as high porosity, high surface to volume ratio, and extracellular matrixmimicking structure, [2] extraordinarily electromagnetism, electrospun nanofibers and their assemblies have been created substantial interests from various research fields, including clothing, [3] environmental filter, [4][5][6] battery, [7] and tissue-engineered scaffolds. [8][9][10] However, the chaotic motion of electrospun nanofibers due to bending instability has hampered the accurate and precise control in constructing nanofiber assemblies and generally created randomly interwoven 2D nanofiber mat. [11] Recent advances in the electrospinning process have expanded the range of the nanofiber assemblies from a 2D flat nanofiber mat to a 3D nanofibrous macrostructure.…”

Section: Introductionmentioning

confidence: 99%

Conformal Fabrication of an Electrospun Nanofiber Mat on a 3d Ear Cartilage-shaped Hydrogel Collector Based on Hydrogel-Assisted Electrospinning

Song

Ryu

Lee

et al. 2021

Preprint

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Electrospinning is a common and versatile process to produce nanofibers and deposit them on a collector as a two-dimensional nanofiber mat or a three-dimensional (3D) macroscopic arrangement. However, 3D electroconductive collectors with complex geometries, including protruded, curved, and recessed regions, generally caused hampering of a conformal deposition and incomplete covering of electrospun nanofibers. In this study, we suggested a conformal fabrication of an electrospun nanofiber mat on a 3D ear cartilage-shaped hydrogel collector based on hydrogel-assisted electrospinning. To relieve the influence of the complex geometries, we flattened the protruded parts of the 3D ear cartilage-shaped hydrogel collector by exploiting the flexibility of the hydrogel. We found that the suggested fabrication technique could significantly decrease an unevenly focused electric field, caused by the complex geometries of the 3D collector, by alleviating the standard deviation by more than 70% through numerical simulation. Furthermore, it was experimentally confirmed that an electrospun nanofiber mat conformally covered the flattened hydrogel collector with a uniform thickness, which was not achieved with the original hydrogel collector. Given that this study established the conformal electrospinning technique on 3D electroconductive collectors, it will contribute to various studies related to electrospinning, including tissue engineering, drug/cell delivery, environmental filter, and clothing.

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Reconstruction of in vivo-like in vitro model: Enabling technologies of microfluidic systems for dynamic biochemical/mechanical stimuli

Cited by 23 publications

References 149 publications

Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation

Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation

Advances in Engineering Human Tissue Models

Conformal Fabrication of an Electrospun Nanofiber Mat on a 3d Ear Cartilage-shaped Hydrogel Collector Based on Hydrogel-Assisted Electrospinning

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