Reconstruction of in-vivo subthreshold activity of single neurons from large-scale spiking recordings

Abstract

Current developments in the manufacturing of silicon probes allow recording of spikes from large populations of neurons from several brain structures in freely moving animals. It is still, however, technically challenging to record the membrane potential from awake behaving animals. Routine access to the subthreshold activity of neurons would be of great value in order to understand the role of, for example, neuronal integration, oscillations, and excitability. Here we have developed a framework for reconstructing the subthreshold activity of single neurons using the spiking activity from large neuronal populations. The reconstruction accuracy and reliability have been evaluated with ground truth data provided from simultaneous patch clamp membrane potential recordings in-vivo. Given the abundance of large-scale spike recordings in the contemporary systems neuroscience society, this approach provides a general access to the subthreshold activity and hence could shed light on the intricate mechanisms of the genesis of spiking activity.

Cortical activity at different time scales: high‐pass filtering separates motor planning and execution

“…We regarded a unit as a single unit when the number of spikes within an inter-spike interval of less than 2 ms corresponded to a smaller ring rate than the average ring rate of the unit. To de ne the degree of decorrelation across neurons, we used the m-rate 29 . The m-rate denotes the minimum spike rate in the spike-triggered spike average between two neurons (cross correlogram).…”

“…The subthreshold reconstruction algorithm, SubLab, has been described in detail elsewhere 29 . In short, the algorithm uses the spikes of one unit (target unit) to reconstruct its subthreshold activity by means of the spiking activity of the remaining units (input units).…”

“…Paw velocities provide a general measure of movement magnitude independent of speci c types of movements. To allow a comparison of the discretized and typically low frequency spike trains of sensorimotor cortex with the continuous paw movements, we reconstructed the continuous subthreshold activity with a resolution of 10 ms from the spiking activity 29 (Fig. 4B).…”