Reconstruction of lymph follicles in the mouse popliteal lymph node after X-irradiation damage.

Histological observations on rat popliteal lymph nodes after blockage of their afferent lymphatics.

“…The lymph follicle comprises a solid accumulation of small lymphocytes, the majority of which are B lymphocytes (HOWARD et al, 1972;GOLDSCHNEIDER and MCGREGOR, 1973). The small lymphocytes, or B lymphocytes, accumulate in the follicles, because the stroma of the follicles has a capacity to collect this type of lymphocytes, as indicated by our previous observations (HOSHI et al, 1984b). A sublethal dose of whole body X-irradiation to the mouse caused marked destruction of lymphocytes in the lymph node, and the lymph follicles in the node became free of lymphocytes.…”

Effect of Sublethal X-irradiation on Follicular Trapping of Immune Complexes in the Mouse Lymph Node

“…Following X-irradiation, lymphocytes are extensively destroyed throughout the body, radioresistant FDC and a very small number of B lymphocytes being left behind in each residual follicle in the lymphoid organs (ICinet-Denoel et al, 1982;Humphrey et aL, 1984;Hoshi et aL, 1984;Chen et al, 1995). In this study, therefore, 3-4 semi-serial sections were prepared from each popliteal node taken from irradiated mice, and 2-3 semi-serial sections were stained for persisting FDC and B lymphocytes: the presence of peristing PDC in residual follicles was assesed either by the demonstration of complement receptors, CR1, or by the in vitro trapping assay, or both.…”

“…Alternative explanation for possible ineffectiveness of persisting FDC to trap localized IC in residual follicles is that sublethal irradiation may have directly affected the activity of FDC in lymph node follicles. FDC have been shown to be radio-resistant (Hunter et aL, 1969;Brown et aL, 1973;Humphrey et aL, 1984;Hoshi et aL, 1984;Chen et aL, 1995). However, It is evident that FDC in lymph node follicles were partly damaged by sublethal irradiation.…”

Morphological Changes in the Mouse Popliteal Lymph Node after Local Injection of Dextran Sulfate

“…Destruction of lymph follicles and germinal centers due to damage of lymphoid elements has been observed in lymphoid organs of animals after systemic administration of adrenocorticosteroid hormones and after wholebody x-irradiation. In these cases, the stromata of injured follicles remain little affected, and lymph follicles and germinal centers are reconstructed in the sites of the persisting stromata, when regenerated lymphocytes begin to repopulate the lymphoid organ (Durkin and Thorbecke 1972; Hoshi et al 1984b). The stroma of a lymph follicle consists of follicular dendritic cells (FDC) which interact with B cells.…”