Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review

Abstract

Nuclear transfer procedures have been recently applied for clinical and research targets as a novel assisted reproductive technique and were used for increasing the oocyte activity during its growth and maturation. In this review, we summarized the nuclear transfer technique for germinal vesicle stage oocytes to reconstruct the maturation of them. Our study covered publications between 1966 and August 2017. In result utilized germinal vesicle transfer techniques, fusion, and fertilization survival rate on five different mammalian species are discussed, regarding their potential clinical application. It seems that with a study on this method, there is real hope for effective treatments of old oocytes or oocytes containing mitochondrial problems in the near future.

State of the art of nuclear transfer technologies for assisting mammalian reproduction

“…In contrast, in vitro maturation protocols have been intensely explored in most domestic animals, allowing the in vitro production of embryos from oocytes to become standard practice. Mouse, rabbit, cat, cow, and pig blastocysts have been produced after GV‐NT, achieving births in some of these species (reviewed by Darbandi et al, 2017). In humans, meiosis resumption was observed in vitro after GV‐NT, and the development of ICSI embryos up to the morula stage was reported (Takeuchi et al, 2001).…”

Prospects of Germline Nuclear Transfer in Women With Diminished Ovarian Reserve

“…During GVT, the nucleus of a GV oocyte is being transferred in an enucleated GV donor oocyte ( Figure 1 ). The reconstructed oocyte needs to undergo in vitro maturation before it can be fertilized by the sperm ( 45 ). In ST, the spindle-chromosome complex (containing the DNA) of an MII oocyte is transferred to an enucleated (DNA free) MII donor oocyte which is subsequently fertilized by the sperm of the patient’s partner ( 46 ) ( Figure 1 ).…”