Reconstruction of rat calvarial defects with human mesenchymal stem cells and osteoblast-like cells in poly-lactic-co-glycolic acid scaffolds

Zong, Chen; Xue, Deting; Yuan, Wenji; Wang, W; Shen, Dan; Tong, Xiangming; Shi, Dongyan; Liu, L; Zheng, Qiang; Gao, Changyou; Wang, J

doi:10.22203/ecm.v020a10

Cited by 99 publications

(75 citation statements)

References 46 publications

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“…Color images available online at www.liebertpub.com/tea in vitro. The issue around whether to transplant predifferentiated or undifferentiated MSCs for bone regeneration has been controversial, and related studies show conflicting results, either from the superior potential of bone regeneration using hMSCs in an undifferentiated state, 28 or with the reverse result that differentiated MSCs resulted in greater efficacy in bone formation as compared with undifferentiated cells. 29,30 Nevertheless, the undifferentiated state of hMSCs has attractive properties over hMSCs in a differentiated state, including secretion of trophic factors or the great potential for the differentiation into cells of injury tissue type.…”

Section: Fig 7 Observation Of Immune Cells (Macrophage and T Cells)mentioning

confidence: 99%

In VivoGene Activity of Human Mesenchymal Stem Cells After Scaffold-Mediated Local Transplantation

Hwang

Cho

Kim

2014

Tissue Engineering Part A

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Functional activation of stem cells after transplantation is a main concern in stem cell therapy. For local transplantation, mesenchymal stem cells (MSCs) are usually administered via scaffolds, either by direct implantation or after preculturing of cells, and it is unclear which is better for the activation of transplanted cells. In this study, we investigated the in vivo gene expression activity of human MSCs (hMSCs) transplanted into calvarial defects either directly post-seeding on collagen sponges (Group 1) or after overnight in vitro culturing post-seeding (Group 2). Real-time reverse transcription-polymerase chain reaction at days 7 and 14 after transplantation identified a time-dependent, rapid decrease in gene expression by the hMSCs, which in Group 1 was slightly more attenuated than in Group 2. Both groups exhibited a limited range of human-specific gene expression, which involved type I collagen (ColI), fibronectin, stromal cell-derived factor (SDF-1), and osteoprotegerin. Among these, ColI expression was the most efficient, with higher levels in Group 1 than Group 2. There was a lack of evidence for the expression of osteoblast differentiation-related markers or trophic factors, while resident cells showed clear expression of those genes. Rat-specific b-actin expression in Group 2 was least among the scaffold control, Group 1, and Group 2, and this pattern was repeated in the expression of other rat osteogenic genes. Group 1 transplants positively influenced the osteogenic process of the defect tissue in part, and rat IGF-1 expression was significantly increased in Group 1. This tendency of gene expression by hMSCs in a rat model was very similar to what was observed in transplantations using immunodeficient mice. The current study showed that a main gene expressed by transplanted hMSCs during the initial weeks following transplantation is ColI, with a lack of differentiation-related markers or growth factor expression by hMSCs. Our data suggest that direct transplantation of hMSCs loaded on a collagen sponge is more efficient for gene activation in transplanted hMSCs, and more favorable to the local host tissue than transplantation after preculturing of cells.

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Section: Fig 7 Observation Of Immune Cells (Macrophage and T Cells)mentioning

confidence: 99%

In VivoGene Activity of Human Mesenchymal Stem Cells After Scaffold-Mediated Local Transplantation

Hwang

Cho

Kim

2014

Tissue Engineering Part A

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“…Critical bone defects and fracture non-union remain major challenges in current clinical practice (Hofmann et al 2008;Zong et al 2010). From a patient's perspective, the ultimate goal is replacement of their damaged/lost bone with autogenous material, harvested with minimal donor side morbidity.…”

Section: Introductionmentioning

confidence: 99%

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Kirkham

et al. 2014

Cell Tissue Res

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Translational research in bone tissue engineering is essential for 'bench to bedside' patient benefit. However, the idea combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study was to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 and 40 wt% of Bioglass ® particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy (SEM) showed that PDLLA/Bioglass ® porous scaffolds supported ADSCs attachment, growth and osteogenic differentiation, which were confirmed by enhanced alkaline phosphatase activity (ALP). Higher Bioglass ® content of the PDLLA foams increased more ALP activity compared to PDLLA only group. Extracellular matrix deposition after 8 weeks in in vitro culture was evident by Alcian blue/Sirius red staining.In vivo bone formation was assessed using scaffold/ADSCs constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks.Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass ® constructs compared to the PDLLA only group. This study indicated the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass ® composite scaffold with a high potential stem cells source -human ADSCs could be a promising approach for bone regeneration in clinical setting.

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“…The gene expression level was normalized to that of the respective GAPDH expression. *, P < 0.05. able approaches for the repair or regeneration of damaged bone [22,23]. Tissue engineering and regenerative medicine have shown great promise in promoting bone repair using MSC sheets alone or in combination with implants wrapped in MSC sheets [13,24].…”

Section: Discussionmentioning

confidence: 99%

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

Wang

Guo

et al. 2011

Chin. Sci. Bull.

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A composite construct comprising of a bone mesenchymal stem cell (BMSC) sheet, plasmid DNA, encoding human bone morphogenic protein-2 (hBMP-2), and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) sponge was designed and employed in the restoration of rat calvarial defects. To improve gene transfection efficiency, a cationic chitosan derivative, N,N,N,-trimethyl chitosan chloride (TMC), was employed as the vector. The TMC/DNA complexes had a transfection efficiency of 13% in rat BMSCs, resulting in heterogeneous hBMP-2 expression in a 10-d culture period in vitro. In vivo culture of the composite constructs was performed by implantation into rat full-thickness calvarial defects, using constructs lacking pDNA-hBMP-2 or BMSC sheets as controls. Significantly higher heterogeneous expression of hBMP-2 was detected in vivo at 2 weeks for the cell sheet/DNA complex/scaffold constructs, compared with the constructs lacking pDNA-hBMP-2 or BMSC sheets. New bone formation was evident as early as 4 weeks in the experimental constructs. At 8 weeks, partial bridging of calvarial defects was observed in the experimental constructs, which was significantly better than the constructs lacking pDNA-hBMP-2 or BMSC sheets. Therefore, the combination of the PLGA/HA scaffold with BMSC sheets and gene therapy vectors is effective at enhancing bone formation.gene therapy, bone regeneration, bone marrow stem cells, poly(lactide-co-glycolide), BMP-2 Citation:Li D, Wang W, Guo R, et al. Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes.

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Reconstruction of rat calvarial defects with human mesenchymal stem cells and osteoblast-like cells in poly-lactic-co-glycolic acid scaffolds

Cited by 99 publications

References 46 publications

In VivoGene Activity of Human Mesenchymal Stem Cells After Scaffold-Mediated Local Transplantation

In VivoGene Activity of Human Mesenchymal Stem Cells After Scaffold-Mediated Local Transplantation

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

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