Reconstruction of the High Stigma Exsertion Rate Trait in Rice by Pyramiding Multiple QTLs

Tan, Qi; Bu, Suhong; Chen, Guodong; Yan, Zhenguang; Chang, Zengyuan; Zhu, Haitao; Yang, Weifeng; Zhan, Penglin; Lin, Shaojun; Xiong, Liang; Chen, Songliang; Liu, Guifu; Liu, Zupei; Wang, Shaokui; Zhang, Guiquan

doi:10.3389/fpls.2022.921700

Cited by 15 publications

(16 citation statements)

References 68 publications

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“…The stigma exsertion of weedy rice, promoting the likelihood of outcrossing in nature between weedy and cultivated rice in the same area (Figure 4), created high t values (Tables 2 and 3). The rice genotype exhibiting this trait, together with male sterile ability, represents a breeding goal for the hybrid rice production system (Bakti and Tanaka 2019; Tan et al 2021, 2022; Zou et al 2020). The genetic diversity revealed in this study is higher than that previously reported by Prathepha (2011) ( H E = 0.598) and Cao et al (2006) ( H E = 0.313).…”

Section: Resultsmentioning

confidence: 99%

The genetic diversity and population structure of weedy rice in northeast Thailand accessed by SSR markers

Ponsen,

Loasatit,

Monkham

et al. 2023

Weed Sci

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Thailand’s northeast (NE) region is an area of high-quality cultivated rice (Oryza sativa L.) production. However, an outbreak of weedy rice has recently spread throughout the region. Weedy rice is phenotypically and morphologically similar to cultivated rice, making identification difficult. The prospective management of weedy rice in the future will involve the study of its genetic diversity and population structure in this region. This study assesses the genetic diversity and population structure of 380 weedy rice samples in the northeast of Thailand through simple sequence repeat (SSR) markers. Thirty-one SSR markers generated 213 alleles with an average of 6.87 per locus and an overall gene diversity of 0.723. Based on its geographical origin, weedy rice in the Southern NE showed greater gene diversity than that in the Central NE and Northern NE areas. The out-crossing rate in all regions was relatively high, with the highest being in the Southern NE at 9.769%. According to genetic distance analysis, the clustering of weedy rice samples in Northeast Thailand was not associated with the geographical region. Neighbor-joining and principal coordinate analysis revealed that the 380 weedy rice samples fell into two major clusters. Cluster I contained three weedy rice samples and four wild. In Cluster II, 377 weedy rice samples were closely related to the four cultivated rice cultivars as well as brownbeard rice (Oryza rufipogon Griffiths) wild species. The results suggest that weedy rice in Northeast Thailand may have originated as a cross between cultivated and wild rice, as seen in the closely related species, O. rufipogon. Overall, the findings of this study demonstrate the high genetic diversity of weedy rice in this region. Notably, some samples adapted, performing more like cultivated rice, which may be problematic for the future production of high-quality rice in this region. The prevention of weedy rice should, therefore, be given greater consideration in future studies.

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Section: Resultsmentioning

confidence: 99%

The genetic diversity and population structure of weedy rice in northeast Thailand accessed by SSR markers

Ponsen,

Loasatit,

Monkham

et al. 2023

Weed Sci

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“…The phenotypic analysis showed that the increase of SER did not lead to the change of grain shape in most pyramiding lines. The results indicated that the high-SER trait can be reconstructed by pyramiding of the QTLs in rice ( Tan et al., 2022b ). In this study, we found that grain shape is another factor affecting rice SER, which affects SER by partially affecting stigma shape ( Figures 6 and 7 ).…”

Section: Discussionmentioning

confidence: 99%

“…Seven SSSLs, W17-46-40-10-07-05, W23-07-06-05-02-02, W12-11-22-03-03-163, W02-08-08-08-01, W07-07-02-03-02, W23-19-06-07-19-03 and W09-38-60-07-18-04 carrying respectively fgr , qBLAST-11 , gs3 , gs3 , gw5 , GW7 and gw8 were selected from the HJX74-SSSL library ( Wang et al., 2012 ; Zhang, 2021 ). The pyramiding lines carrying 2-4 QTLs for SER, 2QL-1, 2QL-5, 3QL-3, 3QL-10, 4QL-1 and 4QL-3, were previously constructed by pyramiding QTLs for SER from HJX74-SSSLs through maker assisted selection (MAS) ( Tan et al., 2022b ).…”

Section: Methodsmentioning

confidence: 99%

“…The results showed that the SER can be improved with increasing QTLs in pyramiding lines. The pyramiding lines carrying 5-6 QTLs showed as high SER as wild rice ( Tan et al., 2022b ). The results indicate that SER is a complex trait controlled by a series of QTLs, and the high SER trait can be reconstructed by pyramiding of the QTLs in rice.…”

Section: Introductionmentioning

confidence: 99%

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Grain shape is a factor affecting the stigma exsertion rate in rice

et al. 2023

Self Cite

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Stigma exsertion rate (SER) is an index of outcrossing ability in rice and is a key trait of male sterile lines (MSLs) in hybrid rice. In this study, it was found that the maintainer lines carrying gs3 and gs3/gw8 showed higher SER. Single-segment substitution lines (SSSLs) carrying gs3, gw5, GW7 or gw8 genes for grain shape and gene pyramiding lines were used to reveal the relationship between grain shape and SER. The results showed that the grain shape regulatory genes had pleiotropic effects on SER. The SERs were affected by grain shapes including grain length, grain width and the ratio of length to width (RLW) not only in low SER background, but also in high SER background. The coefficients of determination (R2) between grain length and SER, grain width and SER, and grain RLW and SER were 0.78, 0.72, and 0.91 respectively. The grain RLW was the most important parameter affecting SER, and a larger grain RLW was beneficial to stigma exsertion. The pyramiding line PL-gs3/GW7/gw8 showed the largest grain RLW and the highest SER, which will be a fine breeding resource. Further research showed that the grain shape regulatory genes had pleiotropic effects on stigma shape, although the R2 values between grain shape and stigma shape, and stigma shape and SER were lower. Our results demonstrate that grain shape is a factor affecting SER in rice, in part by affecting stigma shape. This finding will be helpful for breeding MSLs with high SER in hybrid rice.

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“…In a recent study, multiple QTLs were pyramided in an attempt to rebuild the high stigma exsertion rate (SER) in rice. The study found 18 QTLs linked to SER, and the outcomes demonstrated that rice could successfully improve the SER trait by pyramiding several QTLs [ 100 ].…”

Section: Breeding Techniques For Producing Hybrid Ricementioning

confidence: 99%

Hybrid Rice Production: A Worldwide Review of Floral Traits and Breeding Technology, with Special Emphasis on China

Ashraf,

Ghouri,

Baloch

et al. 2024

Plants

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Rice is an important diet source for the majority of the world’s population, and meeting the growing need for rice requires significant improvements at the production level. Hybrid rice production has been a significant breakthrough in this regard, and the floral traits play a major role in the development of hybrid rice. In grass species, rice has structural units called florets and spikelets and contains different floret organs such as lemma, palea, style length, anther, and stigma exsertion. These floral organs are crucial in enhancing rice production and uplifting rice cultivation at a broader level. Recent advances in breeding techniques also provide knowledge about different floral organs and how they can be improved by using biotechnological techniques for better production of rice. The rice flower holds immense significance and is the primary focal point for researchers working on rice molecular biology. Furthermore, the unique genetics of rice play a significant role in maintaining its floral structure. However, to improve rice varieties further, we need to identify the genomic regions through mapping of QTLs (quantitative trait loci) or by using GWAS (genome-wide association studies) and their validation should be performed by developing user-friendly molecular markers, such as Kompetitive allele-specific PCR (KASP). This review outlines the role of different floral traits and the benefits of using modern biotechnological approaches to improve hybrid rice production. It focuses on how floral traits are interrelated and their possible contribution to hybrid rice production to satisfy future rice demand. We discuss the significance of different floral traits, techniques, and breeding approaches in hybrid rice production. We provide a historical perspective of hybrid rice production and its current status and outline the challenges and opportunities in this field.

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Reconstruction of the High Stigma Exsertion Rate Trait in Rice by Pyramiding Multiple QTLs

Cited by 15 publications

References 68 publications

The genetic diversity and population structure of weedy rice in northeast Thailand accessed by SSR markers

The genetic diversity and population structure of weedy rice in northeast Thailand accessed by SSR markers

Grain shape is a factor affecting the stigma exsertion rate in rice

Hybrid Rice Production: A Worldwide Review of Floral Traits and Breeding Technology, with Special Emphasis on China

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