Reconstruction of the ovary microenvironment utilizing macroporous scaffold with affinity-bound growth factors

Felder, Shani; Masasa, Hila; Orenbuch, Ayelet; Levaot, Noam; Goldenberg, Michal Shachar; Cohen, Smadar

doi:10.1016/j.biomaterials.2019.03.013

Cited by 35 publications

(30 citation statements)

References 68 publications

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“…[ 152a ] Owing to the chemical versatility of scaffold building blocks, synthetic biomaterials provide a highly comprehensive tools to create defined cell microenvironments and at largest extent maintain native tumor morphogenesis in 3D cell cultures. [ 78,154 ] Interestingly, designer peptide hydrogels are the upfront representatives in synthetic biomaterials available.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

“…The follicles formed in vitro reach antral size and secreted hormones at levels leading to restoration of ovarian function. [ 154 ] Largely due to technical limitations, the adhesion‐mediated signaling processes are still poorly characterized in ovarian cancer. [ 150 ] Based on the advances in bioengineering cell models in cancer biology, stem cell biology, and regenerative medicine, designer self‐assembling peptide hydrogels can increasingly cater to practical 3D cell‐patterning technologies in a relevantly physiological cell culture manner.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

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Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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mouse intestinal stem cells and primary colorectal cancer cells can be propagated in vitro long term, [1] there is an urgent need to develop more physiologically relevant, efficient, and robust precise oncology models that closely recapitulate the genetic and morphological heterogeneous composition and mimic the arrangement pattern of cancer cells in the original tumor. [2] To our knowledge in biomedical research, hydrogel is the best tool to reconstruct precise oncology models in vitro, especially tumor organoid, which not only recapitulates in vivo biology and microenvironmental factors, but also at large extent allows side-by-side comparison to evaluate the translational potential of 3D model systems to the patients. [2a,3] Generally, hydrogels are mainly composed of hydrophilic polymeric scaffolds that absorb large amounts of water, so the hydrogel matrix better mimics elastic and viscoelastic properties in ECM and microscale topographies of cell matrices, which controls proper cell morphology, directs viable cell behaviors, and drives in vivo fundamental cell-cell or cell-ECM interactions. [4] To recapitulate pathophysiological features of human tumors and imitate various aspects of human tumorigenesis in vivo, hydrogels can provide a realistic platform to establish a useful bridge between in vitro assays and in vivo cell microenvironments. In current biomedical applications, there are many kinds of hydrogels to be developed to mimic the biological properties of ECM, such Designer self-assembling peptides form the entangled nanofiber networks in hydrogels by ionic-complementary self-assembly. This type of hydrogel has realistic biological and physiochemical properties to serve as biomimetic extracellular matrix (ECM) for biomedical applications. The advantages and benefits are distinct from natural hydrogels and other synthetic or semisynthetic hydrogels. Designer peptides provide diverse alternatives of main building blocks to form various functional nanostructures. The entangled nanofiber networks permit essential compositional complexity and heterogeneity of engineering cell microenvironments in comparison with other hydrogels, which may reconstruct the tumor microenvironments (TMEs) in 3D cell cultures and tissue-specific modeling in vitro. Either ovarian cancer progression or recurrence and relapse are involved in the multifaceted TMEs in addition to mesothelial cells, fibroblasts, endothelial cells, pericytes, immune cells, adipocytes, and the ECM. Based on the progress in common hydrogel products, this work focuses on the diverse designer self-assembling peptide hydrogels for instructive cell constructs in tissue-specific modeling and the precise oncology remodeling for ovarian cancer, which are issued by several research aspects in a 3D context. The advantages and significance of designer peptide hydrogels are discussed, and some common approaches and coming challenges are also addressed in current complex tumor diseases.

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Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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“…[50][51][52][53] Besides ECM molecules, other natural polymers widely researched in the field of tissue engineering have also been studied recently for GF delivery. Among these are alginate, a polysaccharide extracted from the cell wall of brown seaweed that grows in cold water regions, [54][55][56] chitosan, another polysaccharide, produced by controlled deacetylation of chitin, [57] and silk fibroin (SF), one of the two main constituent proteins in silk produced by certain arthropods. [58] Alginate exhibits excellent biocompatibility and biodegradability, as well as an ability to be readily processed for 3D scaffolding materials.…”

Section: Conventional Controlled Release Systemsmentioning

confidence: 99%

“…[52] A recent representative example of this type of systems is the combination of unmodified alginate with alginate sulfate, which presents an increased negative charge conferring higher affinity to multiple GFs, to develop an ovary mimetic microenvironment. [56] This system could retain BMP-4 by electrostatic interactions and, in turn, support the culture and growth of porcine primordial follicles seeded with supporting ovarian cells. These scaffolds were able to sustain the development of the follicles up to the preantral stage, with five times more developing follicles and estrogen production than the groups with soluble BMP-4.…”

Section: Conventional Controlled Release Systemsmentioning

confidence: 99%

“…More significant still is the ability of these devices to restore ovarian function after xenotransplantation in ovariectomized severe combined immunodeficiency (SCID) mice, providing the first successful example of GF presentation for this purpose. [56] Despite the continued improvements in this type of controlled release systems, there are various aspects of previously described GF signaling mechanisms that are not taken into consideration. They constitute an advanced delivery system for recombinant versions of GFs, allowing a more sustained and prolonged release to circumvent some problems of soluble bolus administration.…”

Section: Conventional Controlled Release Systemsmentioning

confidence: 99%

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Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Nanotheranostics in Personalized Veterinary Medicine

Ghosh,

Chowdhury,

Kumar

2024

Nanotechnology Theranostics in Livestock Diseases and Management

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Reconstruction of the ovary microenvironment utilizing macroporous scaffold with affinity-bound growth factors

Cited by 35 publications

References 68 publications

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Nanotheranostics in Personalized Veterinary Medicine

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