Recovery and Calibration Techniques: Toward Quantitative Microdialysis

Lange, E

doi:10.1007/978-1-4614-4815-0_2

Cited by 34 publications

(41 citation statements)

References 45 publications

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“…If the measures of adding albumin or changing tubing material do not solve the problem, it is recommended not to perform microdialysis. More detailed recommendation on how to address the problem with adsorption can be found in the AAPS-FDA workshop white paper (16), as well as in (18,24,57,58). However, in the paper by Nirogi et al, a 60-min pre-equilibration was used, which may hide possible adsorption (58).…”

Section: In Vitro Preparations and Adsorption To Tubings And Probementioning

confidence: 99%

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Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Hammarlund‐Udenaes

2017

AAPS J

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Abstract.Microdialysis has contributed with very important knowledge to the understanding of target-specific concentrations and their relationship to pharmacodynamic effects from a systems pharmacology perspective, aiding in the global understanding of drug effects. This review focuses on the historical development of microdialysis as a method to quantify the pharmacologically very important unbound tissue concentrations and of recent findings relating to modeling microdialysis data to extrapolate from rodents to humans, understanding distribution of drugs in different tissues and disease conditions. Quantitative microdialysis developed very rapidly during the early 1990s. Method development was in focus in the early years including development of quantitative microdialysis, to be able to estimate true extracellular concentrations. Microdialysis has significantly contributed to the understanding of active transport at the blood-brain barrier and in other organs. Examples are presented where microdialysis together with modeling has increased the knowledge on tissue distribution between species, in overweight patients and in tumors, and in metabolite contribution to drug effects. More integrated metabolomic studies are still sparse within the microdialysis field, although a great potential for tissue and disease-specific measurements is evident.

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Section: In Vitro Preparations and Adsorption To Tubings And Probementioning

confidence: 99%

“…The references presented here are not complete but give a picture of the area (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Hammarlund‐Udenaes

2017

AAPS J

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“…For collection of pharmaceutical solutes, different in vivo calibration procedures exist and require solute steady state concentrations typically achieved with drug infusions. 41 For endogenous solutes such as the adipokines that may not exist at steady-state concentrations, in vivo calibration methods are not available to determine the true extracellular space concentration. For this reason, we chose to compare total tissue concentrations to the dialysate concentrations.…”

Section: Resultsmentioning

confidence: 99%

In vivo microdialysis sampling of adipokines CCL2, IL-6, and leptin in the mammary fat pad of adult female rats

2014

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Adipocytes from white adipose tissue secrete cytokines and other bioactive proteins which are collectively termed adipokines. Adiposity has been linked with increased breast cancer risk as adipokines secreted by adipocytes significantly affect epithelial cells from which breast cancer arises. Measurement of extracellular adipokine concentrations that would be involved in signaling through mammary tissue is therefore of importance. In this work, microdialysis sampling was used to collect adipokines from the interstitial space of the mammary fat pad of female rats under isoflurane anesthesia. The adipokines CCL2 (MCP-1), leptin and IL-6 were quantified from dialysate samples and compared to total tissue concentrations surrounding the implanted probes. After three hours of microdialysis sampling at 1 μL min-1, the respective median values for these adipokines in dialysate samples were approximately 175 pg mL-1, 150 pg mL-1 and 750 pg mL-1. Adipokine protein levels from dialysates were an order of magnitude lower than levels obtained directly from mammary tissue. However, the adipokine concentrations between excised tissue surrounding the microdialysis sampling probes and control tissue without implants did not differ. This work demonstrates the utility of microdialysis sampling to quantify mammary gland adipokine levels, with relevance to understanding mammary physiology.

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“…Relative recoveries: %-gain and %-loss were estimated as described by Lange et al 16 using the following equations:…”

Section: In-vitro Microdialysis Experimentsmentioning

confidence: 99%

Untitled

2017

IJPSR

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Diphenhydramine hydrochloride (DPH) is an antihistamine used topically to relieve skin itching and, at higher doses, DPH also has the capability to induce local skin anesthesia. The present study investigates the possibility to deliver DPH via iontophoresis using a stainless steel electrode. In-vitro iontophoresis was performed using 1% (w/v) DPH dissolved in glycerin (100%), deionized (DI) water (100%) and a mixture of both (50:50) through porcine ear skin and Franz cell assembly. The applied electrical current was 0, 100, 200, and 300 µA/cm 2 for 60 min. Of the solvents tested, the 100% glycerin provided a consistent increase in DPH skin concentrations with current density and no skin damage. Therefore, the 100% glycerin was selected for in-vivo iontophoresis in a rabbit model at the same three current densities for 60 min. DPH skin concentrations were measured in skin via microdialysis for 240 min. Plasma to skin distribution of DPH was studied following short IVinfusions of 6, 8 and 10 mg/Kg in rabbits. Skin and plasma samples were collected for 420 min. No skin damage was observed even at the higher current density. DPH skin concentrations were higher following iontophoresis than IV-infusion. Thus, iontophoresis of DPH in glycerin from a stainless steel patch may be a safe, effective, and inexpensive way to deliver high dose of DPH topically to the skin.

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Recovery and Calibration Techniques: Toward Quantitative Microdialysis

Cited by 34 publications

References 45 publications

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

In vivo microdialysis sampling of adipokines CCL2, IL-6, and leptin in the mammary fat pad of adult female rats

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