Recovery and fertilization of bovine follicular oocytes

Shea, Brian F.; Janzen, R.E.; McAlister, R.J.

doi:10.1016/0093-691x(83)90094-8

Cited by 7 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present experiment we used the heifer as an intermediate recipient, and a surgical approach similar to that used by Trounson et al (1977) and Shea et al (1983). Oviducts from other species have been used as incubators (rabbit: Sirard & Lambert, 1986;sheep: Critser et al, 1986), as it is believed that a 'block' of early cleavage, probably at the 8-16-cell stage in cattle, will occur during in-vitro culture ) unless a cell-co-culture system is used.…”

Section: Discussionmentioning

confidence: 99%

Pregnancy resulting from cattle oocytes matured and fertilized in vitro

Xu¹,

Greve²,

Callesen³

et al. 1987

Reproduction

121

View full text Add to dashboard Cite

Follicular oocytes (n = 81) collected from cattle at a local slaughterhouse were matured and fertilized in vitro. Of 27 ova 19 (70%) were penetrated by spermatozoa and 40/54 (74%) inseminated ova transferred surgically to the oviducts of a synchronized heifer were recovered by non-surgical flushing of the uterine horns 6 days later. Of the 40 ova 15 (38%) were at the morula, early blastocyst or diminutive morula stages. Culture in vitro sustained further development of all embryos and 9 were expanding or expanded blastocysts. One pregnancy resulted from non-surgical transfer of 2 blastocysts. The results demonstrate that immature oocytes from cattle can be matured and fertilized in vitro, subsequently develop to the blastocyst stage, and develop into a normal pregnancy after non-surgical transfer.

show abstract

Section: Discussionmentioning

confidence: 99%

Pregnancy resulting from cattle oocytes matured and fertilized in vitro

Xu¹,

Greve²,

Callesen³

et al. 1987

Reproduction

121

View full text Add to dashboard Cite

show abstract

“…This opens up many possibilities for correlative studies, such as raising clones, the transfer of genes and the detailed study of early embryonic development (Leibfried-Rutledge et al, 1989;Rexroad, 1989;Gagné et al, 1990). These methods also make it possible to produce calves from superior cows with interesting traits, but which are infertile for nongenetic reasons (Shea et al, 1983). Development rates of IVM-IVF bovine oocytes in different cell culture systems have been described in previous reports (Berg & Brem, 1989 …”

Section: Introductionmentioning

confidence: 99%

Pregnancy rates and births after unilateral or bilateral transfer of bovine embryos produced in vitro

Reichenbach¹,

Liebrich²,

Berg³

et al. 1992

Reproduction

View full text Add to dashboard Cite

In vivo development of in vitro fertilized bovine oocytes matured in vivo versus in vitro

Greve¹,

Xu²,

Callesen³

et al. 1987

J Assist Reprod Genet

View full text Add to dashboard Cite

In vivo developmental potentials of in vivo and in vitro matured oocytes fertilized in vitro were assessed in cattle. One-cell stages produced from in vivo matured oocytes developed into a pregnancy when transferred to the ampulla part of oviducts of synchronized heifers. In vitro matured oocytes achieved high penetration and cleavage rates but did not develop into pregnancies when transferred to synchronized heifers.

show abstract

Recovery and fertilization of bovine follicular oocytes

Cited by 7 publications

References 7 publications

Pregnancy resulting from cattle oocytes matured and fertilized in vitro

Pregnancy resulting from cattle oocytes matured and fertilized in vitro

Pregnancy rates and births after unilateral or bilateral transfer of bovine embryos produced in vitro

In vivo development of in vitro fertilized bovine oocytes matured in vivo versus in vitro

Contact Info

Product

Resources

About