Recovery and prevalence of antibiotic-resistant Salmonella from fresh goat meat in Arusha, Tanzania

Abstract

Meat products are clearly associated with foodborne pathogens including, antibiotic-resistant strains. Population growth and growing consumer demand facilitate the transmission of foodborne pathogens, particularly in developing countries. To determine the prevalence of antibiotic-resistant Salmonella in goat meat, a study was done in Tanzania (June to July, 2015). Overall 120 goat meat samples were collected from five large and five small slaughter facilities (n = 60, respectively). Pre-enrichment for Salmonella isolation was done in Tryptic Soy Broth followed by selective enrichment in Modified Semisolid Rappaport-Vassiliadis agar. Isolation of Salmonella was done in xylose-Lysine-Deoxycholate agar followed by biochemical confirmation in triple sugar iron agar. The average prevalence of Salmonella was 60 and 63% in large and small facilities, respectively. Breakpoint assays indicated an overall low prevalence of resistance (2 to 4%; n = 219 isolates) to ampicillin, amoxicillin, streptomycin, sulphamethoxazole and trimethoprim with complete susceptibility to ciprofloxacin, ceftazidime and cefotaxime. No significant difference (p > 0.05) in the prevalence of resistance between large and small facilities was observed. High probability of Salmonella contamination of goat meat from Arusha area of Tanzania can pose risks to consumers. Antibiotic resistance appears minimal in this population. Improved hygienic slaughter and meat-handling practices are encouraged to reduce the burden of Salmonella-positive meat products.

Molecular Characterization of Multidrug-Resistant and Extended-Spectrum β-Lactamases-Producing Salmonella enterica Serovars Enteritidis and Typhimurium Isolated from Raw Meat in Retail Markets

“…The prevalence also varied among different upazilas, with the highest prevalence recorded at 50% in Kanaighat and Gowainghat, and the lowest prevalence at 11.76% in Osmaninagar. The prevalence of Salmonella in goat meat was determined to be 18.10%, which was lower than the findings reported in a study from Pakistan [23] and higher than those from studies conducted in India, Modjo, Bishoftu, Arusha (Tanzania), and Gujarat (India) based on goat carcass swabs [27][28][29]. In the present study, S. Typhimurium was identified in cattle meat as the dominant serovar among other Salmonella spp.…”

“…The reported prevalence rates of Salmonella in goat meat varies globally, with rates as high as 60% reported in Pakistan [23]. The detection of Salmonella was reported from goat meat swabs in different regions of different countries such as a rate of 8.3% in Modjo (Ethiopia), 7.5% in Bishoftu (Ethiopia), 4% in Arusha (Tanzania), and 3.5% in Gujrat (India) [27][28][29].…”

Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh

“…The overall prevalence rate of Salmonella spp. in retail goat meat was found to be 18.10 % in this study, which is higher to the previous studies where the prevalence of Salmonella from goat carcass swab was 9 % at India,8.3 % at Modjo and 7.5 % at Bishoftu, 4 % at Arusha (Tanzania), 3.5 % at Gujrat in India ( Gaspary et al, 2016 ; Makwana et al, 2015 ; Naik et al, 2015 ; Woldemariam et al, 2005 ). The high prevalence might be related to the wet market collecting of meat samples from goat slaughtered in unsanitary settings and eviscerated in contaminated regions with intestinal contents.…”

Non-typhoidal Salmonella contributes to gastrointestinal infections in Morogoro: Evidence from patients attending Morogoro regional referral hospital in Tanzania

“…In Tanzania, there are few reports of the occurrence of Salmonella spp. infection in both humans and animals [ 10 – 12 ] and Salmonella infection in Tanzania, concretely in Morogoro, may be higher than expected. Healthcare services in low- and middle-income countries are facing significant challenges due to antimicrobial multi-drug resistance, which is increasing rapidly due to increased global human mobility and irrational use of antimicrobials in the health and livestock sectors [ 13 ].…”