Recovery and purification of Aspergillus niger phytase from crude extract using AOT / isooctane reversed micelles

Neira-Vielma, Alberto A.; Iliná, Anna; Álvarez, Georgina Michelena; Nascimento, Cynthia O.; Martínez-Hernández, José L.; Carneiro-da-Cunha, Maria das Graças

doi:10.1016/j.btre.2020.e00471

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…The molecular weight of A. niger CFR 335 phytase was around 66 kDa. 11,24 Neira-Vielma et al 11 found that the molecular weight of phytase produced by A. niger was 89 kDa as demonstrated by SDS-PAGE.…”

Section: Purification and Molecular Weight Determination Of Aspergillmentioning

confidence: 99%

“…Since monogastric animals such as pigs lack phytase enzyme, they excrete large amounts of phosphorus in the environment in the form of phytate, leading to pollution by this element. 11 Additionally, phytate binds non-selectively to proteins and amino acids and thus inhibits the digestive enzymes like trypsin and αamylase, causing a reduction of protein digestibility in animals. Phytic acid binds to amino acids such as histidine, arginine and lysine, forming protein-phytate complexes.…”

Section: Introductionmentioning

confidence: 99%

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Purification and Characterization of Phytase Enzyme Extracted from Aspergillus niger SF58 and its Application in Cereal Dephytinization

2021

TJNPR

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Phytases are the group of enzymes that catalyze phytate hydrolysis and release a utilizable form of inorganic phosphorus. So, they are used to improve the nutritive value of animal feed. The current study aims to purify, characterize the phytase enzyme, and to study its application in cereal dephytinization. Molecular identification of the fungal isolate was achieved using the 18S rRNA and phytase activity was determined using phytic acid as substrate. Phytase from Aspergillus niger SF58 was purified using dialysis and Sephadex G 100 column chromatography to get enzyme recovery and purification fold of 72.74% and 2.303, respectively. The purity of purified phytase was confirmed through SDS-PAGE technique which showed a one protein band corresponding to phytase activity at 22 kDa. The optimum reaction time for phytase activity was revealed to be 15 min, whereas the optimal temperature and pH values were found to be 60 o C and pH 5.5. The purified enzyme was able to retain 46.93% of its initial activity after 4 months of storage at 4 o C. Metal ions (Co 2+ , Cu 2+ , Mn 2+ , Fe 2+ , Zn +2) acted as activators for the purified phytase, whereas Mg 2+ , Ni 2+ and Al 3+ served as inhibitors. The purified phytase showed the highest substrate specificity toward wheat bran, followed by the wheat flour, soybean , and corn-flower, respectively. The use of Aspergillus niger SF58 phytase in dephytinization of wheat bran and soybean indicated the possibility of applying the enzyme to increase the nutritional values of feed and food additives.

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Section: Purification and Molecular Weight Determination Of Aspergillmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Phytase Enzyme Extracted from Aspergillus niger SF58 and its Application in Cereal Dephytinization

2021

TJNPR

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“…Theoretically, the more reverse micelles there are in a given concentration range, the better the decolorization efficiency. In reversed micelle system, the concentration of employed surfactant is the pivotal factor for the number of reverse micelles (Neira-Vielma et al, 2020). Therefore, the CTAB dosage is an important parameter in the n-hexanol-CTAB-isooctane reversed micelle system.…”

Section: Effect Of Ctab Concentrationmentioning

confidence: 99%

“…Liquid–liquid extraction with reversed micelle system has been used in the extraction and purification of polar biomolecules for recent decades, due to its high selectivity, fast mass transfer, relatively low cost, and easy scale‐up. The method is usually applied to separate proteins, enzymes, nucleic acids, organic acids, and cells (Kilikian et al, 2000; Neira‐Vielma et al, 2020). As polar macromolecules, polysaccharides probably be extracted from crude and colored polysaccharides through a reverse micelle system, and their decolorization would be realized.…”

Section: Introductionmentioning

confidence: 99%

A preliminary study on preparation, characterization, and prebiotic activity of a polysaccharide from the edible mushroom Ramaria flava

Zhou

Wang

et al. 2022

Journal of Food Biochemistry

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Polysaccharides derived from edible mushrooms were sources of new prebiotic compounds. Limited studies of their prebiotic effects, as well as the presence of residual dark colors, impede their use as prebiotics in the food industry. To boost the prebiotic value of polysaccharides from the edible mushroom Ramaria flava, a decolorization method, and the physicochemical characterization and prebiotic potential of the decolorized polysaccharide (DRFP) were investigated in this study. The reversed micelle system consisting of n-hexanol/isooctane (3:7, v/v) and 200 mM surfactant (CTAB) was an appropriate decolorized method for R. flava crude polysaccharide. That decolorized polysaccharide was 101.68 kDa and contained glucose, galactose, mannose,

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“…A utilização do Sistema de Duas Fases Aquosas (SDFA) tem se destacado como uma abordagem promissora para a extração e purificação de enzimas de interesse biotecnológico, como as fitases (Neira-Vielma et al,2020). As fitases são enzimas que desempenham um papel crucial na hidrólise do ácido fítico (Pramitha et al, 2021), um antinutriente presente em alimentos vegetais, principalmente grãos e sementes, e que interfere na absorção de minerais essenciais pelos animais (Samtiya et al, 2020).…”

Section: Introductionunclassified

Qué Deben Saber Los Estudiantes De La Nematología Agrícola

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2023

Avances Científicos Y Tecnológicos en Ciencias Agrícolas

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Editora Direitos para esta edição cedidos à Atena Editora pelos autores. Open access publication by Atena Editora Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterála de nenhuma forma ou utilizá-la para fins comerciais.Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético. Conselho Editorial Ciências Agrárias e Multidisciplinar

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Recovery and purification of Aspergillus niger phytase from crude extract using AOT / isooctane reversed micelles

Cited by 10 publications

References 35 publications

Purification and Characterization of Phytase Enzyme Extracted from Aspergillus niger SF58 and its Application in Cereal Dephytinization

Purification and Characterization of Phytase Enzyme Extracted from Aspergillus niger SF58 and its Application in Cereal Dephytinization

A preliminary study on preparation, characterization, and prebiotic activity of a polysaccharide from the edible mushroom Ramaria flava

Qué Deben Saber Los Estudiantes De La Nematología Agrícola

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