“…Frontiers in Bioengineering and Biotechnology frontiersin.org proteins, such as fragment crystallizable (Fc)-fusion proteins and mAbs, can undergo several post-translational modifications (proteolysis, glycosylation, among others), making their accurate bioprocessing and, consequently, their characterization, difficult to achieve (Jenkins et al, 2008). Moreover, biopharmaceuticals are more unstable and sensitive to the conditions employed during the manufacturing process, storage, and handling, such as temperature and pH (Kesik-Brodacka, 2018;Tavares et al, 2019;Lin et al, 2021). Since biopharmaceuticals are highly prone to denaturation and degradation events, as well as to modifications in their amino acid and sugar patterns, the manufacturing conditions must be strictly controlled (Kesik-Brodacka, 2018;Lin et al, 2021).…”