Recovery Based Nanowire Field-Effect Transistor Detection of Pathogenic Avian Influenza DNA

Abstract

Fast and accurate diagnosis is critical in infectious disease surveillance and management. We proposed a DNA recovery system that can easily be adapted to DNA chip or DNA biosensor for fast identification and confirmation of target DNA. This method was based on the re-hybridization of DNA target with a recovery DNA to free the DNA probe. Functionalized silicon nanowire field-effect transistor (SiNW FET) was demonstrated to monitor such specific DNA-DNA interaction using high pathogenic strain virus hemagglutinin 1 (H1) DNA of avian influenza (AI) as target. Specific electric changes were observed in real-time for AI virus DNA sensing and device recovery when nanowire surface of SiNW FET was modified with complementary captured DNA probe. The recovery based SiNW FET biosensor can be further developed for fast identification and further confirmation of a variety of influenza virus strains and other infectious diseases. #

Surface composition and interactions of mobile charges with immobilized molecules on polycrystalline silicon nanowires

“…Device structure and electrical characteristics of the poly-SiNW FET N-type poly-SiNW FETs fabricated using previously reported methods by us [1][2][3][4][5][6][7][21][22][23][24] were used as the nano-transducers in this study. The poly-SiNW was defined by a side-wall spacer technique [24] in a 6-inch Si wafer capped with a 100 nm SiO 2 layer and a 50 nm Si 3 N 4 layer.…”

“…An n-type field-effect transistor, comprising two poly-SiNWs that served as conducting channels and that had dimensions of 100 nm width and 1.6 m length, was fabricated using the sidewall spacer technique [4][5][6][7][21][22][23][24]. This technique is compatible with current commercial semiconductor process technologies and has been developed by our team, with an emphasis on applications in aqueous solutions [3][4][5][6][7][22][23][24][25].…”

“…Many silicon nanowire field-effect transistor base biosensors have been developed for the ultra-high detection of a variety of proteins, nucleic acids and small molecules [1][2][3][4][5][6][7][8][9]. Its low power requirement, mass production potential, and integrability with other electronic components make the poly-SiNW FET a highly attractive device in the fast-growing diagnostic industry.…”

Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

“…Single-stranded viral nucleic acids may exist in positive (+) sense, i.e., directly translatable, or negative (−) sense, i.e., complementary polarity. Therefore, a variety of BioFEDs (mostly based on SiNW-FETs or CNT-FETs) have been developed for detecting nucleic acid sequences of different viruses directly or after reverse transcription, including the genomic RNAs of influenza A virus [(−)ssRNA] (Lin et al, 2009(Lin et al, , 2012Kao et al, 2011;Thu et al, 2013;Karnaushenko et al, 2015;Tran et al, 2017), dengue virus [(+)ssRNA] (Zhang et al, 2010;Nuzaihan et al, 2016Nuzaihan et al, , 2018, or (+)ssRNA of hepatitis C virus (Dastagir et al, 2007), and the partially ds DNA of hepatitis B viruses (Wu et al, 2014;Lu et al, 2016). These BioFEDs are highly sensitive with detection limits often in the pM range, although a detection limit in the fM range was reported for ultrasensitive SiNW-FETs as well (Lin et al, 2009;Wu et al, 2014;Nuzaihan et al, 2016).…”

Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe

“…The silicon nanowire (NW) field-effect transistor (FET), as a biosensor, promises ultra-high sensitivity in the detection of a variety of proteins 1 , nucleic acids 2 – 4 , and metal cations 5 . Owing to the advantages of real-time, label-free, selectivity, and sensitivity detection capabilities, the FET-based detection technique continually receives significant attention.…”