Recovery Dynamics of Intestinal Bacterial Communities of CCl4-Treated Mice with or without Mesenchymal Stem Cell Transplantation over Different Time Points

Abstract

Liver injury has caused significant illness in humans worldwide. The dynamics of intestinal bacterial communities associated with natural recovery and therapy for CCl4-treated liver injury remain poorly understood. This study was designed to determine the recovery dynamics of intestinal bacterial communities in CCl4-treated mice with or without mesenchymal stem cell transplantation (i.e., MSC and CCl4 groups) at 48 h, 1 week (w), and 2 w. MSCs significantly improved the histopathology, survival rate, and intestinal structural integrity in the treated mice. The gut bacterial communities were determined with significant changes in both the MSC and CCl4 groups over time, with the greatest difference between the MSC and CCl4 groups at 48 h. The liver injury dysbiosis ratio experienced a decrease in the MSC groups and a rise in the CCl4 groups over time, suggesting the mice in the MSC group at 48 h and the CCl4 group at two weeks were at the least gut microbial dysbiosis status among the corresponding cohorts. Multiple OTUs and functional categories were associated with each of the bacterial communities in the MSC and CCl4 groups over time. Among these gut phylotypes, OTU1352_S24-7 was determined as the vital member in MSC-treated mice at 48 h, while OTU453_S24-7, OTU1213_Ruminococcaceae, and OTU841_Ruminococcus were determined as the vital phylotypes in CCl4-treated mice at two weeks. The relevant findings could assist the diagnosis of the microbial dysbiosis status of intestinal bacterial communities in the CCl4-treated cohorts with or without MSC transplantation.

Characteristics of the gut microbiota in Bifidobacterium catenulatum LI10 pretreated rats with lower levels of D-galactosamine-induced liver damage

“…Bacteroides has been determined as both beneficial and pathogenic bacteria (Wexler, 2007). For example, Bacteroides vulgatus and B. dorei were found to prevent coronary artery disease (Yoshida et al, 2018), while toxin from B. fragilis was a driver of colonic disease (Valguarnera & Wardenburg, 2020). Some other phylotypes assigned to Frisingicoccus, Muribaculaceae, Romboutsia and Turicibacter were determined as representative ASVs to Non-Severe cohort but not Severe cohort.…”

“…A group of phylotypes were determined to maintain the stability of gut microbiota network in Non‐Severe cohort based on CoNet and fragmentation analyses. The two analyses have been used to explore the microbiota network and structural gatekeeper in the previous studies (Wagner Mackenzie et al, 2017; Xu et al, 2020; Zha, Chen, et al, 2020). ASV135_Lachnospiraceae_NK4A136 was determined as the most powerful structural gatekeeper with most correlations in the microbiota network of Non‐Severe cohort.…”