Recovery from chilling modulates the acyl-editing of phosphatidic acid molecular species in barley roots (Hordeum vulgare L.)

Vilchez, Ana Carolina; Margutti, Micaela Peppino; Reyna, Matias; Wilke, Natalia; Villasuso, Ana Laura

doi:10.1016/j.plaphy.2021.09.005

Cited by 11 publications

(25 citation statements)

References 41 publications

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“…Figure 1B shows that the UI for CC is the lowest and that this parameter increases during stress and decreases when the stress is released, in agreement with the trend in GP. It has to be noted, however, that phytosterols were not determined in Vilchez et al 15 and that these lipids are also important for defining the structure of interfacial water. Zeta Potential and Hydrodynamic Size.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…This is because modifications in membrane viscosity can trigger signaling events, leading to whole-cell adaptation to fluctuations in temperature, including the synthesis of protective proteins and lipids that may alter the membrane's properties in the long term. 10 In our previous study, 15 we analyzed a wide range of glycerolipid molecular species associated with glycerolipid remodeling in barley roots during exposure to cold temperatures and short-and long-term recovery. The lipidomic profile showed an increment in total lipids and in the unsaturation index (UI) during cold stress and a partial restoration of both during recovery.…”

Section: ■ Introductionmentioning

confidence: 99%

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Biophysical Properties of Lipid Membranes from Barley Roots during Low-Temperature Exposure and Recovery

Vilchez,

Villasuso,

Wilke

2023

Langmuir

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Glycerolipid remodeling, a dynamic mechanism for plant subsistence under cold stress, has been posited to affect the biophysical properties of cell membranes. In barley roots, remodeling has been observed to take place upon exposure to chilling stress and to be partially reverted during stress relief. In this study, we explored the biophysical characteristics of membranes formed with lipids extracted from barley roots subjected to chilling stress, or during a subsequent short- or long-term recovery. Our aim was to determine to what extent barley roots were able to offset the adverse effects of temperature on their cell membranes. For this purpose, we analyzed the response of the probe Laurdan inserted in bilayers of different extracts, the zeta potential of liposomes, and the behavior of Langmuir monolayers upon compression. We found important changes in the order of water molecules, which is in agreement with the changes in the unsaturation index of lipids due to remodeling. Regarding Langmuir monolayers, we found that films from all the extracts showed a reorganization at a surface pressure that depends on temperature. This reorganization occurred with an increase in entropy for extracts from control plants and without entropy changes for extracts from acclimated plants. In summary, some membrane properties were recovered after the stress, while others were not, suggesting that the membrane biophysical properties play a role in the mechanism of plant acclimation to chilling. These findings contribute to our understanding of the impact of lipid remodeling on biophysical modifications in plant roots.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Biophysical Properties of Lipid Membranes from Barley Roots during Low-Temperature Exposure and Recovery

Vilchez,

Villasuso,

Wilke

2023

Langmuir

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“…Interestingly, higher activity of acyl-CoA:lysophospholipid Acyl editing is also known to occur in response to stress, such as temperature stress, salt stress, and nutrient deprivation. For instance, lipidomic profiling during chilling stress in barley roots revealed that as particular molecular species of PC decreased, those molecular species increased in PA (Vilchez et al, 2021). Transcriptional analysis of genes involved in acyl editing revealed that the barley LPCAT gene was upregulated within five hours of cold recovery, and phospholipase D activity rose during cold treatment and after five hours of cold recovery (Vilchez et al, 2021).…”

Section: Mechanisms Of Acyl Editing and Acyl Flux Through Pc Into Tagmentioning

confidence: 99%

“…For instance, lipidomic profiling during chilling stress in barley roots revealed that as particular molecular species of PC decreased, those molecular species increased in PA ( Vilchez et al., 2021 ). Transcriptional analysis of genes involved in acyl editing revealed that the barley LPCAT gene was upregulated within five hours of cold recovery, and phospholipase D activity rose during cold treatment and after five hours of cold recovery ( Vilchez et al., 2021 ). Thus, it is likely that PC is hydrolyzed to produce PA during cold stress and recovery, and that acyl editing of PC plays an important role in chilling stress and recovery.…”

Section: Mechanisms Of Acyl Editing and Acyl Flux Through Pc Into Tagmentioning

confidence: 99%

Do betaine lipids replace phosphatidylcholine as fatty acid editing hubs in microalgae?

Hoffmann

Shachar‐Hill²

2023

Front. Plant Sci.

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Acyl editing refers to a deacylation and reacylation cycle on a lipid, which allows for fatty acid desaturation and modification prior to being removed and incorporated into other pools. Acyl editing is an important determinant of glycerolipid synthesis and has been well-characterized in land plants, thus this review begins with an overview of acyl editing in plants. Much less is known about acyl editing in algae, including the extent to which acyl editing impacts lipid synthesis and on which lipid substrate(s) it occurs. This review compares what is known about acyl editing on its major hub phosphatidylcholine (PC) in land plants with the evidence for acyl editing of betaine lipids such as diacylglyceryltrimethylhomoserine (DGTS), the structural analog that replaces PC in several species of microalgae. In land plants, PC is also known to be a major source of fatty acids and diacylglycerol (DAG) for synthesis of the neutral lipid triacylglycerol (TAG). We review the evidence that DGTS contributes substantially to TAG accumulation in algae as a source of fatty acids, but not as a precursor to DAG. We conclude with evidence of acyl editing on other membrane lipid substrates in plants and algae apart from PC or DGTS, and discuss future analyses to elucidate the role of DGTS and other betaine lipids in acyl editing in microalgae.

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“…PA is present in small (at most a few percent) amounts in biomembranes but increases rapidly in plants after a stress stimulus [ 11 , 12 ]. This is followed by a decline in PA concentration and a subsequent increase in DGPP production as PA is converted to DGPP by phosphatidic acid kinase (PAK) [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

The Electrostatic Basis of Diacylglycerol Pyrophosphate—Protein Interaction

Graber

Kwarteng

Lange

et al. 2022

Cells

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Diacylglycerol pyrophosphate (DGPP) is an anionic phospholipid formed in plants, yeast, and parasites under multiple stress stimuli. It is synthesized by the phosphorylation action of phosphatidic acid (PA) kinase on phosphatidic acid, a signaling lipid with multifunctional properties. PA functions in the membrane through the interaction of its negatively charged phosphomonoester headgroup with positively charged proteins and ions. DGPP, like PA, can interact electrostatically via the electrostatic-hydrogen bond switch mechanism but differs from PA in its overall charge and shape. The formation of DGPP from PA alters the physicochemical properties as well as the structural dynamics of the membrane. This potentially impacts the molecular and ionic binding of cationic proteins and ions with the DGPP enriched membrane. However, the results of these important interactions in the stress response and in DGPP’s overall intracellular function is unknown. Here, using 31P MAS NMR, we analyze the effect of the interaction of low DGPP concentrations in model membranes with the peptides KALP23 and WALP23, which are flanked by positively charged Lysine and neutral Tryptophan residues, respectively. Our results show a significant effect of KALP23 on the charge of DGPP as compared to WALP23. There was, however, no significant effect on the charge of the phosphomonoester of DGPP due to the interaction with positively charged lipids, dioleoyl trimethylammonium propane (DOTAP) and dioleoyl ethyl-phosphatidylcholine (EtPC). Divalent calcium and magnesium cations induce deprotonation of the DGPP headgroup but showed no noticeable differences on DGPP’s charge. Our results lead to a novel model for DGPP—protein interaction.

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Recovery from chilling modulates the acyl-editing of phosphatidic acid molecular species in barley roots (Hordeum vulgare L.)

Cited by 11 publications

References 41 publications

Biophysical Properties of Lipid Membranes from Barley Roots during Low-Temperature Exposure and Recovery

Biophysical Properties of Lipid Membranes from Barley Roots during Low-Temperature Exposure and Recovery

Do betaine lipids replace phosphatidylcholine as fatty acid editing hubs in microalgae?

The Electrostatic Basis of Diacylglycerol Pyrophosphate—Protein Interaction

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