Recovery, identification and relationships by microsatellite analysis of ancient grapevine cultivars from Castilla-La Mancha: the largest wine growing region in the world

Mena, A.; Martínez, J.; Fernández-González, M.

doi:10.1007/s10722-013-0064-3

Cited by 27 publications

(18 citation statements)

References 32 publications

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“…Recently, with the name of "Grumer," we have identified some accessions from the Alicante province that do not correspond to "Valencí" but grouped with "Muscat Istambul" [61]. Lacombe et al [62] proposed as the origin of "Muscat Istambul" the cross of "Muscat of Alexandria" Â "Valencí Blanc" which was confirmed by Mena et al [63]. Therefore, it could be easy to found this homonym.…”

Section: Richness Of Grapevine Germplasm In the Comunitat Valencianamentioning

confidence: 78%

“…The comparison of the SSR profile of the "Esclafagerres" accessions to SSR profiles in the VIVC database (including 3265 accessions), those in the International Vitis database (including 3430 accessions), as well as those reported in several publications [62,63,69] did not match with any of the included varieties. No matches were found when the SSR profile of "Esclafagerres" was blasted to the Italian Vitis database.…”

Section: Determining the Ssr Profilesmentioning

confidence: 98%

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Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Gisbert¹,

Peiró²,

Pedro³

et al. 2018

Grapes and Wines - Advances in Production, Processing, Analysis and Valorization

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A great number of varieties have been described in grapevine; however, few of them are currently in use. The increasing concern on varietal diversity loss has encouraged actions for recovering and preserving grapevine germplasm, which represents valuable resources for breeding as well as for diversification in grapevine-derived products. On the other hand, it is expected that this important crop, which is distributed in warm areas worldwide, will suffer the climate changes. Therefore, it is also convenient the identification of intravarietal variability and the recovery of accessions well adapted to particular environments. In this chapter, we will contribute to highlight the importance of recovering ancient materials, the usefulness of SSR markers to determine their molecular profile, the importance to analyze their virus status, and the possibilities that offer biotechnological tools for virus sanitation and in vitro storage as a complement of field preservation. In this context, we have evaluated different grapevine accessions and developed in vitro culture protocols for micropropagation, sanitation, and storage grapevine cultivars. In this work, we report the results obtained for the historic variety "Valencí Blanc" (or "Beba") and the historic and endangered variety "Esclafagerres" ("Esclafacherres" or "Esclafacherris").

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Section: Richness Of Grapevine Germplasm In the Comunitat Valencianamentioning

confidence: 78%

Section: Determining the Ssr Profilesmentioning

confidence: 98%

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Gisbert¹,

Peiró²,

Pedro³

et al. 2018

Grapes and Wines - Advances in Production, Processing, Analysis and Valorization

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“…Variedad posiblemente utilizada para uva de mesa, cultivada en Israel y en Túnez (Galet, 2000) y presente también en la Península Ibérica dónde ha sido localizada en la región de Castilla La Mancha (Mena et al, 2014). Los datos de pedigrí avalan está distribución dado que desciende de la hibridación de la variedad Moscatel de Alejandría y la variedad ibérica Beba o Valencí Blanco distribuidas ambas en esas regiones.…”

Section: Moscatel De Estambulunclassified

Identificación de poblaciones de vid silvestre y parras guiadas sobre las fachadas de los caseríos de la Cuadrilla de Ayala (Álava, España).

Hidalgo¹,

Martı́nez-Zapater²,

Ocete³

et al. 2018

Munibe, Cienc. nat.

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ResumenEl presente trabajo se ha centrado sobre la Cuadrilla de Ayala (Álava), donde se han localizado ocho poblaciones relictas de vid silvestre en diversos hábitats. Se ha comprobado su carácter dioico durante el periodo de floración. Se han identificado las principales especies parásitas existentes sobre estas parras y las principales componentes de su vegetación acompañante. Por otro lado, se han estudiado quince vides cultivadas, con una edad supe- rior a cincuenta años, situadas en la fachada principal de los caseríos. El análisis del ADN de ambos tipos de parras se ha realizado con 48 marcadores SNPs. Los resultados obtenidos han sido comparados con la base de datos disponible en el Instituto de Ciencias de la Vid y del Vino (Logroño), a fin de proceder a la caracterización genética de las muestras.Palabras clave: Chacolí, estado sanitario, marcadores SNP, vegetación acompañante, Vitis vinífera subsp. sylvestris (C.C. Gmelin) Beger & Hegi. AbstractThis paper focuses on the Cuadrilla de Ayala (Álava province, Spain), where eight relic wild grapevine populations were located in different habitats. The dioecious nature of these lianas was determined at flowering time. The main parasitic species on these vines and also the most frequent taxa of their accompanying vegetation were identified. On the other hand, fifteen vines, aged in excess of fifty, and located in the main entrances of traditional farmhouses (caseríos), were also studied. The analysis of the DNA from both kinds of sampled vines was based on 48 SNPs markers. Results were compared with the available data base at the Instituto de Ciencias de la Vid y del Vino (Logroño, Spain) for the sample genetic characterization. LaburpenaHonako lan hau Aiaraldean (Araba) kokatu da. Habitat desberdinetan bizi diren zortzi basamahats-gune aurkitu dira, desagertzeko puntuan. Loraldian dioikoak direla egiaztatu da. Mahats-parra horietan dauden espezie parasito gehienak identifikatu dira eta bai beraiekin batera bizi diren gainerako landare laguntzaileak ere.Bestalde, hamabost mahats-landare landu ikertu dira, berrogeita hamar urtetik gorakoak, baserrietako aurrealdeko horman hazi direnak. Bi mahatsondoen ADN analisia SNP 48 markatzailerekin egin da. Lortutako emaitzak Logroñoko Instituto de Ciencias de la Vid y del Vino-ko datu-basearekin alderatu dira, laginen ezaugarri genetikoak zehazteko.Gako hitzak: txakolina, osasun egoera, SPN markatzaileak, landaredi laguntzailea, Vitis vinifera subsp. sylvestris (C.C. Gmelin) Beger & Hegi.

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“…Recently, with the name of 'Grumer', we have identified some accessions from the Alicante province that do not correspond to 'Valencí' but grouped with 'Muskat Istambul' [61]. Lacombe et al [62] proposed as the origin of 'Muscat Istambul' the cross of 'Muscat of Alexandria' x 'Valencí Blanc' which was confirmed by Mena et al [63]. Therefore, it could be easy to find this homonym.…”

Section: The Historic Varieties 'Valencí Blanc' and 'Esclafagerres'mentioning

confidence: 71%

“…The comparison of the SSR profile of the 'Esclafagerres' accessions to SSR profiles in the VIVC database (including 3,265 accessions); those in the International Vitis database (including 3,430 accessions), as well as with those reported in several publications [62,63,69] did not match with any of the included varieties. No matches were neither found when the SSR profile of 'Esclafagerres' was blasted to the Italian Vitis database.…”

Section: Determining the Ssr Profilesmentioning

confidence: 97%

Desarrollo y aplicación de técnicas biotecnológicas para el saneamiento, multiplicación, caracterización y conservación de germoplasma de vid (Vitis vinifera L.)

Galán¹,

María²

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Grapevine is a crop of vegetative propagation with great economical importance which is distributed worldwide in temperate zones. It is mainly used for wine and table grape production. Despite the fact thousands of varieties exist only few are commonly used, producing a loss of variability. Since the arrival of the phylloxera aphid the grapevine culture is performed using resistant rootstocks. The Directive Commission 2005/43/EC amending the Directive Council annexes 68/193/EEC, indicates that grapevine material should be free of these viruses: Grapevine fanleaf virus (GFLV), Grapevine leafroll associated virus 1 (GLRaV-1), Grapevine leafroll associated virus 3 (GLRaV-3), Grapevine fleck virus (GFkV) and Arabis mosaic virus (ArMV). In this work different methodologies related with in vitro culture were evaluated and developed with the aim of sanitate, mutiplicate and preserve grapevine germplam. In vitro preservation of grapevine varieties is a complementary stratregy of field preservation. In a preservation program it is important the selection of the starting material, determine its sanitary status, sanitate if it is necessary and, establish the appropriate in vitro conditions for its preservation. In the first chapter of this thesis (Chapter 1) the assays carried out for inducing somatic embryogenesis in 16 grapevine varieties which include six that were infected by the viruses GFLV, GLRaV-3 and GFkV are described. In grapevine, somatic embryogenesis has been described as a usefull methodology for virus sanitation and is the common pathway to adventitious regeneration. Efficient regeneration prototocols are necessary to address breeding by genetic transformation. In this work, cut seed are used as starting material and virus-free plants and high precentages of regeneration were obtained in all the varieties evaluated. The use of microsatellite markers has been useful to select those plants with the same genotype than the mother plant. From the other side, in this work, a micropropagation protocol starting from a healthy plant of the cultivar 'Monastrell' was developed and high multiplication rates with good adaptation to field conditions were obtained (Chapter 2). The culture medium MW was evaluated in 16 grapevine varieties as well as the following modifications: reduction in a half of the sugar content and elimination of the Indolebutyric Acid. The aim of these assays was to determine for each variety which conditions are the most appropiate to maintain in active in vitro culture this germplasm, lengthen the time between subcultures for reducing costs (Chapter 3). In Chapter 4 it is included a divulgation report that summarized the steps followed in the MINECO proyect CGL2015-70843-1.5.1.5. Crioconservación/Crioterapia 1.5.2 Micropropagación X 1.5.3 Conservación in vitro 19 1.5.4 Mejora genética y transformación genética 20 2. Objetivos 39 3. Capítulos 41 Capítulo 1: Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants 43 Capítulo 2: In vi...

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Recovery, identification and relationships by microsatellite analysis of ancient grapevine cultivars from Castilla-La Mancha: the largest wine growing region in the world

Cited by 27 publications

References 32 publications

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Identificación de poblaciones de vid silvestre y parras guiadas sobre las fachadas de los caseríos de la Cuadrilla de Ayala (Álava, España).

Desarrollo y aplicación de técnicas biotecnológicas para el saneamiento, multiplicación, caracterización y conservación de germoplasma de vid (Vitis vinifera L.)

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