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The Brazilian pepper tree (Schinus terebinthifolius) is an invasive species that requires significant disturbance to eradicate. Previous studies have identified associations between the Brazilian pepper tree and ectomycorrhizal fungi (EMF). However, limited research has explored the connection between disturbance from removal and the effect on EMF. This study investigated the sensitivity of EMF and the broader fungal community to the full and selective removal of the Brazilian pepper tree. During the selective removal of Brazilian pepper tree, we examined the mycorrhizal community of the Arroyo willow (Salix lasiolepis) to assess the influence of the restoration disturbance on native species. We used ITS2 sequencing to identify the EMF present during the restoration. Our expectation was that both removal efforts would reduce the presence of EMF. Contrary to our predictions, full removal increased EMF richness and relative abundance in the soil. As anticipated, selective removal reduced the richness and relative abundance of EMF associated with soil. Selective removal led to a decrease in the richness of EMF in arroyo willow roots with no effect on relative abundance. Moreover, fungal community composition in soil and roots shifted significantly during selective and full removal. However, the community composition of EMF, specifically, remained constant across treatment types. During full removal efforts, the application of organic soil amendments may have contributed to the increase in the diversity and relative abundance of EMF. Selective removal will require additional measures, such as soil amendments, to curtail the loss of EMF.
The Brazilian pepper tree (Schinus terebinthifolius) is an invasive species that requires significant disturbance to eradicate. Previous studies have identified associations between the Brazilian pepper tree and ectomycorrhizal fungi (EMF). However, limited research has explored the connection between disturbance from removal and the effect on EMF. This study investigated the sensitivity of EMF and the broader fungal community to the full and selective removal of the Brazilian pepper tree. During the selective removal of Brazilian pepper tree, we examined the mycorrhizal community of the Arroyo willow (Salix lasiolepis) to assess the influence of the restoration disturbance on native species. We used ITS2 sequencing to identify the EMF present during the restoration. Our expectation was that both removal efforts would reduce the presence of EMF. Contrary to our predictions, full removal increased EMF richness and relative abundance in the soil. As anticipated, selective removal reduced the richness and relative abundance of EMF associated with soil. Selective removal led to a decrease in the richness of EMF in arroyo willow roots with no effect on relative abundance. Moreover, fungal community composition in soil and roots shifted significantly during selective and full removal. However, the community composition of EMF, specifically, remained constant across treatment types. During full removal efforts, the application of organic soil amendments may have contributed to the increase in the diversity and relative abundance of EMF. Selective removal will require additional measures, such as soil amendments, to curtail the loss of EMF.
Worldwide wetland loss over the past 50 years has made wetland conservation a public policy priority, leading to an increase in wetland restoration programs. However, predicting long‐term restoration outcomes remains difficult. The monitoring of these programs rarely exceeds 5–10 years, forcing wetland managers to rely on short‐term success criteria that may be criticized by the scientific community. Our objective was to assess the significance of four short‐term success criteria (Carex ssp. shoot density, Salix ssp. survival, invasive species cover, and hydrologic dissimilarity to reference sites) used in a restoration program of 12 wetlands monitored for 5 years post‐restoration in predicting restoration outcomes 15 years post‐restoration. We defined the success of restoration efforts after 15 years using a cluster analysis‐based approach, and the clusters were described using principal coordinate analysis and Tukey's post hoc honest significant difference test. Finally, we assessed the pertinence of each short‐term success criteria in predicting long‐term restoration outcomes using Pearson correlation tests and spatial regressive models. Our results demonstrate that stress‐based short‐term success criteria can be reliable predictors of longer‐term success for communities with shallow water tables, whereas target‐species‐based short‐term success criteria are not. Hydrologic dissimilarity to the reference site was appropriate for willow‐sedge community outcome predictions, while invasive species cover was best for sedge community outcome predictions. For communities in drier habitats, such as the willow‐herb community, none of the tested short‐term success criteria were significant predictors of long‐term restoration outcomes, and further research is required to identify suitable short‐term success criteria.
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