Recovery of a New Biogroup of Yersinia ruckeri from Diseased Rainbow Trout (Oncorhynchus mykiss, Walbaum)

Austin, Dawn A.; Robertson, Peter A.; Austin, Brian

doi:10.1078/072320203322337416

Cited by 95 publications

(115 citation statements)

References 9 publications

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“…When Austin et al (2003) examined reference strains of Y. ruckeri in API 20E, they were identified as Hafnia alvei, albeit with a possibility of Y. ruckeri. The fresh isolates were generally equated with H. alvei, but with doubtful profiles, and with a possibility of Y. ruckeri.…”

Section: Distinctiveness Of Api 20e For Fish Bacteriamentioning

confidence: 99%

“…Some authors proceeded with API 20E identification according to the manufacturer's suggestions, be it freshwater or sea fish (Athanassopoulou et al, 1999;Li et al, 1999;Zorilla et al, 1999;Jaksic et al, 2002;Villamil et al, 2003), while some recommended the incubation of API 20E strips for fish isolates at 22°C for 36 hours (Santos et al, 1993), at 27°C for 24 hours (Kozinska et al, 2002), and others (Biosca et al, 1993;Doukas et al, 1998;Arias et al, 2003;Austin et al, 2003;Padros et al, 2006) incubated strips at 25°C and performed readings in 24 and 48 hours. For the identification of sea fish isolates, Grisez et al (1991) suggested the following modifications of the prescribed method for the inoculation of the test strip: increasing of the incubation time to 48-72 hours, lowering the incubation temperature to 26°C, using a suspension of 1.5% saline as inoculum, and allowing only fermentation of sugars by sealing these cups with sterile mineral oil.…”

Section: Adaptation Of Api 20e For Fish Bacterial Isolatesmentioning

confidence: 99%

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Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

Popović¹,

Čož‐Rakovac²,

Strunjak‐Perović³

2007

Vet. Med.

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ABSTRACT:The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as Yersinia ruckeri, Edwardsiella ictaluri, Vibrio anguillarum.

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Section: Distinctiveness Of Api 20e For Fish Bacteriamentioning

confidence: 99%

Section: Adaptation Of Api 20e For Fish Bacterial Isolatesmentioning

confidence: 99%

Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

Popović¹,

Čož‐Rakovac²,

Strunjak‐Perović³

2007

Vet. Med.

View full text Add to dashboard Cite

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“…Gavín et al (2002) concluded that the lateral flagella are mainly responsible for the adhesion process in this species and also for its ability to form biofilm. However, in the case of Yersinia ruckeri, the causal agent of yersiniosis, a non-motile biogroup identified as Y. ruckeri serovar I biotype 2 has been described, which is able to cause the disease in rainbow trout (Austin et al, 2003;Fouz et al, 2006). In fact, Evenhuis et al (2009) have demonstrated that the lack of the flagellum and flagellar secretion machinery do not affect Y. ruckeri virulence.…”

Section: Motilitymentioning

confidence: 99%

An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

Méndez¹,

Reimundo²,

Pérez-Pascual³

et al. 2012

Health and Environment in Aquaculture

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“…Esta infección es producida por Yersinia ruckeri aislada por primera vez, en el Valle Hagerman (USA) a principios del 1950 (Rucker 1966, Ross et al 1966. Además ha sido reportada en Australia (Bullock et al 1977), Iran (Soltani et al 1999), Turquía (Timur & Timur 1991), Portugal (Sousa et al 1996), Sudáfrica (Bragg & Henton 1986, Bragg 1991, China (Raidal et al 2004), Francia (Lesel et al 1983), Reino Unido (Austin et al 2003) entre otros países. En Perú, Y. ruckeri fue aislada de 34 piscifactorías del departamento de Junín en el año 2004 utilizando procedimientos bioquímicos estándares (Bravo & Kojagura 2004).…”

Section: Introductionunclassified

Diagnóstico e identificación rápidos por PCR de Yersinia ruckeri aislada de Oncorhynchus mykiss procedentes de Canta, Lima, Perú

2011

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ResumenSe muestrearon 20 ejemplares (alevines y juveniles) de trucha arco iris cultivados en la piscifactoría Acochinchán (Canta, Lima, Perú), y se les aplico la técnica de la Reacción en Cadena de la Polimerasa (PCR) con la finalidad de obtener una identificación rápida del agente patógeno Yersinia ruckeri que produce la enfermedad entérica de la boca roja (ERM) y genera elevadas tasas de mortalidad. Nueve ejemplares fueron asintomáticos mientras que 11 presentaron signos de ERM. Se aislaron 22 cepas bacterianas del hígado, bazo y riñón. Se empleó la técnica de la PCR para la amplificación y cebadores específicos (ARNr 16S), que permitieron amplificar un fragmento de ADN de 575 pb de Yersinia ruckeri. Diecinueve cepas fueron identificadas como Yersinia ruckeri mediante la PCR, tanto en peces sintomáticos como asintomáticos. Se estableció un tiempo de diagnóstico de 26 horas, en comparación con los 2 ó 3 días que duraría el diagnóstico empleando las pruebas bioquímicas. Palabras Clave: Yersinia ruckeri, Oncorhynchus mykiss, PCR, diagnóstico, Enfermedad Entérica de la BocaRoja. AbstractTwenty individuals of rainbow trout were sampled (fry and juveniles) from Acochinchan Fishfarm (Canta, Lima -Peru), and analyzed with the Polimerase Chain Reaction test (PCR ) in order to achieve a rapid identification of Yersinia ruckeri, which is the pathogen agent that causes the enteric red mouth disease (ERM) and produces high rates of mortality. Nine fish samples were asymptomatic, while 11 of them showed signs of ERM. In addition, 22 bacterial strains were isolated from the liver, spleen and kidney. PCR and specific primers (16S rRNA), were used to amplified a specific 575 bp DNA fragment of Yersinia ruckeri. Nineteen strains were identified as Yersinia ruckeri by PCR in symptomatic and asymptomatic fishes. It was established a diagnosis time of 26 hours, compared with the 2 or 3 days that would take the diagnosis using biochemical tests.

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Recovery of a New Biogroup of Yersinia ruckeri from Diseased Rainbow Trout (Oncorhynchus mykiss, Walbaum)

Cited by 95 publications

References 9 publications

Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

Diagnóstico e identificación rápidos por PCR de Yersinia ruckeri aislada de Oncorhynchus mykiss procedentes de Canta, Lima, Perú

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