Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages

Haas, MaryEllen R; Nguyen, Darlene V.; Shook, Brett A.

doi:10.3390/biomedicines9121752

Cited by 9 publications

(6 citation statements)

References 72 publications

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“…Pairwise colocalization signals on CD26 and CD29 have been reported in healthy mouse myofibroblasts in the past ( 42 ). Being present in most cell types, CD26 plays a double functionality as an immune-regulatory and proteolytic enzyme.…”

Section: Discussionmentioning

confidence: 78%

Using adjusted local assortativity with Molecular Pixelation unveils colocalization of membrane proteins with immunological significance

Rhomberg-Kauert,

Karlsson,

Thiagarajan

et al. 2024

Front. Immunol.

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Advances in spatial proteomics and protein colocalization are a driving force in the understanding of cellular mechanisms and their influence on biological processes. New methods in the field of spatial proteomics call for the development of algorithms and open up new avenues of research. The newly introduced Molecular Pixelation (MPX) provides spatial information on surface proteins and their relationship with each other in single cells. This allows for in silico representation of neighborhoods of membrane proteins as graphs. In order to analyze this new data modality, we adapted local assortativity in networks of MPX single-cell graphs and created a method that is able to capture detailed information on the spatial relationships of proteins. The introduced method can evaluate the pairwise colocalization of proteins and access higher-order similarity to investigate the colocalization of multiple proteins at the same time. We evaluated the method using publicly available MPX datasets where T cells were treated with a chemokine to study uropod formation. We demonstrate that adjusted local assortativity detects the effects of the stimuli at both single- and multiple-marker levels, which enhances our understanding of the uropod formation. We also applied our method to treating cancerous B-cell lines using a therapeutic antibody. With the adjusted local assortativity, we recapitulated the effect of rituximab on the polarity of CD20. Our computational method together with MPX improves our understanding of not only the formation of cell polarity and protein colocalization under stimuli but also advancing the overall insight into immune reaction and reorganization of cell surface proteins, which in turn allows the design of novel therapies. We foresee its applicability to other types of biological spatial data when represented as undirected graphs.

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Section: Discussionmentioning

confidence: 78%

Using adjusted local assortativity with Molecular Pixelation unveils colocalization of membrane proteins with immunological significance

Rhomberg-Kauert,

Karlsson,

Thiagarajan

et al. 2024

Front. Immunol.

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“…Moreover, the regulation of oxidative stress plays an important role in the wound-repair process. Reduced production of reactive oxidative species (ROS) and/or enhanced antioxidant activity may provide faster wound repair and improve disturbed wound healing processes [41]. Therefore, we aimed to determine if plant-based topical formulations can modulate systemic oxidative status after three weeks of daily application.…”

Section: Discussionmentioning

confidence: 99%

Topical Application of Siberian Pine Essential Oil Formulations Enhance Diabetic Wound Healing

Nikolic,

Andjic,

Bradic

et al. 2023

Pharmaceutics

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This study aimed to develop novel topical formulations based on a natural component (0.5% of Siberian pine essential oil) and to assess its wound-healing capacity through macroscopic, histopathological, and biochemical examination. The phytochemical profile of Pinus sibirica essential oil (PSEO) and rheological analysis and safety potential of formulations were determined. The wound-healing effect was evaluated on an excision wound model in diabetic Wistar albino rats randomly divided into the following groups topically treated with (1) untreated, (2) 1% silver sulfadiazine, (3) ointment base, (4) gel base, (5) PSEO ointment, and (6) PSEO gel. Formulations containing PSEO were stable and safe for skin application. Three weeks of treatment with both PSEO formulations (ointment and gel) led to a significant reduction in wound size (98.14% and 96.28%, respectively) and a remarkably higher level of total hydroxyproline content (9.69 µg/mg and 7.26 µg/mg dry tissue, respectively) relative to the control group (65.97%; 1.81 µg/mg dry tissue). These findings were in correlation with histopathological results. Topically applied PSEO formulations were associated with a significant reduction in most of the measured pro-oxidants and enhanced activity of the antioxidant defense system enzymes (p < 0.05). Our findings showed that gel and ointment with PSEO demonstrated significant wound-repairing capabilities in the excision wound model.

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“…High blood glucose activates the advancement of tissue fibrosis, and during diabetic wound healing is linked to reduced and malfunction of myofibroblasts (Deb et al, 2017). To demonstrate the effect of hyperglycaemia on fibroblasts, Haas et al (Haas et al, 2021) noted a decreased expression of fibrosis-related genes and myofibroblasts in diabetic wounds. Lerman et al (Lerman et al, 2003) found a significant reduction in fibroblast cell migration, transformed production of matrix metalloproteinase 9 (MMP-9), and a huge reduction in the production of vascular endothelial growth factor (VEGF) from diabetic mice.…”

Section: Influence Of Hyperglycaemia On Fibroblast Cells During Skin ...mentioning

confidence: 99%

Photobiomodulation (PBM): a therapeutic technique targeting fibroblast cell regeneration and survival in diabetic wounds

Jere,

Houreld

2024

Front. Photonics

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In injury, fibroblasts are key for disintegrating the fibrin clot, secreting collagen proteins, and constructing extracellular matrix Altered fibroblast function is among the common characteristics of delayed wound healing and development of chronic wounds. Thus, treatment methods for effective wound healing require agents that affect the complexed wound milieu, including fibroblast cells. Diabetes mellitus (DM) is a common cause of impaired fibroblast cell function, delayed wound healing, development of chronic ulcers, and limb amputation. Photobiomodulation (PBM) promotes fibroblast cell proliferation, migration, release of growth factors and wound healing. Yet, its use is limited largely due to an incomplete understanding of its underlying mechanisms at the molecular and cellular level. In this review, we summarise the involvement of fibroblast cells in wound healing, and the effect of PBM on fibroblast cell function and diabetic wound healing. PubMed, Google Scholar, and Research Gate were used to find pertinent academic articles.

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Recovery of Altered Diabetic Myofibroblast Heterogeneity and Gene Expression Are Associated with CD301b+ Macrophages

Cited by 9 publications

References 72 publications

Using adjusted local assortativity with Molecular Pixelation unveils colocalization of membrane proteins with immunological significance

Using adjusted local assortativity with Molecular Pixelation unveils colocalization of membrane proteins with immunological significance

Topical Application of Siberian Pine Essential Oil Formulations Enhance Diabetic Wound Healing

Photobiomodulation (PBM): a therapeutic technique targeting fibroblast cell regeneration and survival in diabetic wounds

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