Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals

Brook, Itzhak

doi:10.1128/jcm.26.6.1181-1188.1988

Cited by 108 publications

(44 citation statements)

References 19 publications

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“…During the 10 year study period, 1010 isolates were identified from 518 samples positive for anaerobic bacteria (average = 1.9 isolates/sample; Table 1). These results corroborate the polymicrobial nature of infections caused by anaerobic bacteria [4–6]. On the other hand, and in agreement with other studies [4,7], the majority of the isolates were derived from the abdominal cavity, skin, soft tissues or bones (Table 1).…”

Section: Isolation Source and Recovery Rate Of Anaerobic Bacteria Frsupporting

confidence: 92%

Phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major Costa Rican hospital

Rodríguez-Cavallini

Vargas

Rodríguez

et al. 2011

Clinical Microbiology and Infection

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Because of limitations in infrastructure, the aetiology of infections caused by anaerobic bacteria is seldom determined in clinical laboratories of developing countries. This study reports on the identification of 1010 anaerobic bacterial isolates collected between 1999 and 2008 in a major Costa Rican hospital with the use of two commercial phenotypic systems (RapID 32A and API 20A). Approximately 60% of the isolates were Gram-positive and, among the 35 species of Gram-positive bacteria found, the genera Clostridium, Propionibacterium and Eggerthella, and anaerobic cocci predominated. Twenty eight species were found among 395 isolates of Gram-negative bacteria. Species of Bacteroides were very frequent, followed by species of Prevotella, Veillonella, Fusobacterium and Porphyromonas.

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Section: Isolation Source and Recovery Rate Of Anaerobic Bacteria Frsupporting

confidence: 92%

Phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major Costa Rican hospital

Rodríguez-Cavallini

Vargas

Rodríguez

et al. 2011

Clinical Microbiology and Infection

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“…A number of surveys have looked at the frequency of anaerobes in clinical specimens and found that GPAC constitute 24-31% of all anaerobic isolates (Holland et al, 1977;Wren et al, 1977;Brook, 1988b;Murdoch et al, 1994;Murdoch, 1998;Boyanova et al, 2004;Wildeboer-Veloo et al, 2007;Brazier et al, 2008;Mikamo et al, 2011). They can be isolated from a wide variety of sites, of which the dominating are abscesses and infections of skin and soft tissue, mouth, bone and joint, upper respiratory and female genital tracts.…”

Section: Clinical Relevancementioning

confidence: 99%

“…Peptostreptococcus anaerobius is one of the most common GPAC associated with infections of the abdominal cavity and female urogenitary tract (Brook, 1988b(Brook, , 1989aMurdoch et al, 1994). Infections of the female urogenitary tract include bacterial vaginosis, which is a polymicrobial syndrome, where Lactobacillus populations are replaced by a mixture of bacteria including P. anaerobius, Gram-negative anaerobic rods such as Prevotella spp., the facultative Gardnerella vaginalis and the genital mycoplasmas Mycoplasma hominis and Ureaplasma urealyticum (Hill, 1993).…”

Section: Clinical Importancementioning

confidence: 99%

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

2013

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Among the Gram-positive anaerobic bacteria associated with clinical infections, the Gram-positive anaerobic cocci (GPAC) are the most prominent and account for approximately 25-30% of all isolated anaerobic bacteria from clinical specimens. Still, routine culture and identification of these slowly growing anaerobes to the species level has been limited in the diagnostic laboratory, mainly due to the requirement of prolonged incubation times and time-consuming phenotypic identification. In addition, GPAC are mostly isolated from polymicrobial infections with known pathogens and therefore their relevance has often been overlooked. However, through improvements in diagnostic and in particular molecular techniques, the isolation and identification of individual genera and species of GPAC associated with specific infections have been enhanced. Furthermore, the taxonomy of GPAC has undergone considerable changes over the years, mainly due to the development of molecular identification methods. Existing species have been renamed and novel species have been added, resulting in changes of the nomenclature. As the abundance and significance of GPAC in clinical infections grow, knowledge of virulence factors and antibiotic resistance patterns of different species becomes more important. The present review describes recent advances of GPAC and what is known of the biology and pathogenic effects of Anaerococcus, Finegoldia, Parvimonas, Peptoniphilus and Peptostreptococcus, the most important GPAC genera isolated from human infections.

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“…The members of the group are bile-resistant, anaerobic Gram-negative rods or coccobacilli which are opportunistic pathogens encountered in peritonitis, septicaemia and wound infections. Bacteroides fragilis and B. thetaiotaomicron are the anerobic bacteria most commonly recovered from clinical specimens and are mainly encountered in peritonitis, appendicitis (Brook 1988), colpitis (Lang et al 1980) and inflammation of the gall bladder (Shimada et al 1984). Exceptionally, B. vulgatus can be isolated from clinical material.…”

Section: Introductionmentioning

confidence: 99%

Rapid differentiation of the species of the genus Bacteroides sensu stricto by capillary gas chromatography of cellular carbohydrates

Engelhard

Mutters

1991

Journal of Applied Bacteriology

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Whole-cell hydrolysates of Bacteroides fragilis, the type species of the genus Bacteroides Castellani and Chalmers 1919, and the genetical closely related species B. vulgatus, B. ovatus, B. eggerthii, B. distasonis, B. uniformis, B. thetaiotaomicron, B. stercoris, B. merdae, and B. caccae were used to determine characteristic carbohydrate patterns by capillary gas chromatography. On the basis of the chemical derivatization of the carbohydrates seven characteristic peaks for peracetylated aldononitriles and nine characteristic peaks for peracetylated o-methyloximes were selected from the carbohydrate fingerprints of the reference strains to prepare a dichotomous identification key. The classification of an unknown strain supposed to belong to the formerly called 'Bacteroides fragilis group' is possible with this key. Some of the advantages of the technique were that the identification of Bacteroides fragilis-like strains requires only 4-5 h after primary isolation and that the bacteria can be exposed to oxygen because viability of the organisms is not necessary. Sophisticated anaerobic techniques can therefore be avoided for identification.

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Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals

Cited by 108 publications

References 19 publications

Phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major Costa Rican hospital

Phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major Costa Rican hospital

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

Rapid differentiation of the species of the genus Bacteroides sensu stricto by capillary gas chromatography of cellular carbohydrates

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