Recovery of anammox process performance after substrate inhibition: Reactor performance, sludge morphology, and microbial community

Wan, Kai; Yu, Ye; Hu, Jinggang; Liu, Xuemei; Deng, Xin; Yu, Junxia; Chi, Ruan; Xiao, Chunqiao

doi:10.1016/j.biortech.2022.127351

Cited by 53 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OTU1200 and OTU2434 are, respectively members of the taxa norank_f_norank_ o_ norank_c_OLB14 and Geothrix , both of which play denitrifying roles ( Ma et al, 2021 ). Among the species with lower relative abundance, OTU689 (0.03126%) is a species of SM1A02 , a genus with good nitrification capacities and anammox potential ( Wan et al, 2022 ). As key microbes, these species are assumed to perform essential functions in maintaining the overall functional stability of the system.…”

Section: Resultsmentioning

confidence: 99%

Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Zhong

Pang³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

In this study, the influence of Fe3+ on N removal, microbial assembly, and species interactions in a biofilm system was determined. The results showed that maximum efficiencies of ammonia nitrogen (NH4+-N), total nitrogen (TN), phosphorus (P), and chemical oxygen demand (COD) removal were achieved using 10 mg/L Fe3+, reaching values of 100, 78.85, 100, and 95.8%, respectively, whereas at concentrations of 15 and 30 mg/L Fe3+ suppressed the removal of NH4+-N, TN, and COD. In terms of absolute abundance, the expression of bacterial amoA, narG, nirK, and napA was maximal in the presence of 10 mg/L Fe3+ (9.18 × 105, 8.58 × 108, 1.09 × 108, and 1.07 × 109 copies/g dry weight, respectively). Irrespective of Fe3+ concentrations, the P removal efficiency remained at almost 100%. Candidatus_Competibacter (10.26–23.32%) was identified as the most abundant bacterial genus within the system. Determinism (50%) and stochasticity (50%) contributed equally to microbial community assembly. Co-occurrence network analysis revealed that in the presence of Fe3+, 60.94% of OTUs in the biofilm system exhibited positive interactions, whereas 39.06% exhibited negative interactions. Within the OTU-based co-occurrence network, fourteen species were identified as key microbes. The stability of the system was found to be predominantly shaped by microbial cooperation, complemented by competition for resources or niche incompatibility. The results of this study suggested that during chemical P removal in wastewater treatment plants using biofilm methods, the concentration of supplemental Fe3+ should be maintained at 10 mg/L, which would not only contribute to P elimination, but also enhance N and COD removal.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Zhong

Pang³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The genera in group 1 (G1) ( SBR1031 and C. Brocadia) contributed to nitrogen removal. , Members of group 2 (G2) ( Limnobacter and Anaerolineaceae ) primarily participated in the degradation of complex organic compounds . Among them, the abundance changes of Anaerolineaceae and C.…”

Section: Resultsmentioning

confidence: 99%

ZVI-Mediated High-Rate Nitrogen Removal from Fulvic Acid (FA)-Containing Wastewater by Anammox: Revealing the Genomic and Molecular Level Mechanisms

et al. 2023

View full text Add to dashboard Cite

The anammox-based technologies are generally inhibited by refractory dissolved organic matter (rDOM), which is ubiquitous in real wastewater. In this study, a novel cost-effective approach, namely, zero-valent iron (ZVI) treatment, was presented to alleviate such inhibitory effects. The results showed that ZVI mitigated the inhibition of fulvic acids (FA) to anammox. Due to the 2 g/L ZVI addition, the nitrogen removal efficiency (NRE) increased from 83.53 to 90.06% when the FA concentration increased from 0 to 160 mg/L in wastewater. Additionally, the co-occurrence network linking Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and 16S rRNA sequencing results revealed the mechanism of ZVI mitigating inhibition by promoting the reduction of nitro and nitroso groups of FA and reshaping the metabolic division of functional bacteria. Hydrolytic acidifying bacteria (e.g., Anaerolineaceae and Ignavibacterium) were enriched for FA degradation, which was ultimately beneficial for denitrifying bacteria and anaerobic ammonium oxidation bacteria (AnAOB). Furthermore, metagenomics revealed that ZVI stimulated multiple nitrogen removal formed by anammox, denitrification, and DNRA by accelerating extracellular electron transfer resorting to FA serving as an electron shuttle and upregulating functional genes encoding electron generation, transport, and consumption processes.

show abstract

“…SRTs of 15 and 20 days facilitated microbial hydrolysis involving Caulobacter and Nocardioides, common hydrolytic bacteria. 34 As the dominant AOB, Nitrosomonas was crucial in partial nitrification, 35 showing high translational activity at all SRTs. Only at SRT of 10 days, the population abundance of total and BONCAT+ Nitrobacter was 1.1% and 3.1%, respectively, contributing to NO 3 − -N production in R2.…”

Section: Microbial Community Structure In the P-sndpr System 341 Diff...mentioning

confidence: 99%

“…However, these values were lower than those of Nitrosomonas (p < 0.05), suggesting the P-SNDPR system effectively inhibited NOB growth to achieve stable partial nitrification. SM1A02, a potential anammox strain, 35 was found and exhibited translational activity in R3 (7.21%) and R4 (6.50%) during the light phase (Figure 4). However, only weak anammox activity was detected in this study (Figure S6).…”

Section: Microbial Community Structure In the P-sndpr System 341 Diff...mentioning

confidence: 99%

Combined Phototrophic Simultaneous Nitrification-Endogenous Denitrification with Phosphorus Removal (P-SNDPR) System Treating Low Carbon to Nitrogen Ratio Wastewater for Potential Carbon Neutrality

Meng,

Zeng,

Zhang

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

Conventional biological nutrient removal processes rely on external aeration and produce significant carbon dioxide (CO 2 ) emissions. This study constructed a phototrophic simultaneous nitrification-denitrification phosphorus removal (P-SNDPR) system to treat low carbon to nitrogen (C/N) ratios wastewater and investigated the impact of sludge retention time (SRT) on nutrient removal performance, nitrogen conversion pathway, and microbial structure. Results showed that the P-SNDPR system at SRT of 15 days had the highest nutrient removal capacity, achieving over 85% and 98% removal of nitrogen and phosphorus, respectively, meanwhile maintaining minimal CO 2 emissions. Nitrogen removal was mainly through assimilation at SRTs of 5 and 10 days, and nitrification-denitrification at SRTs of 15 and 20 days. Stable partial nitrification was facilitated by photoinhibition and low DO levels. Flow cytometry sorting technique results revealed SRT drove community structural changes in translational activity (BONCAT+) microbes, where BONCAT+ microbes were mainly simultaneous nitrogen and phosphorus removal bacteria (Candidatus Accumulibacter), denitrifying bacteria (Candidatus Competibacter and Plasticicumulans), ammonia-oxidizing bacteria (Nitrosomonas), and microalgae (Chlorella and Dictyosphaerium). The P-SNDPR system represents a novel, carbon-neutral process for efficient nutrient removal from low C/N ratio wastewater without aeration and external carbon source additions.

show abstract

Recovery of anammox process performance after substrate inhibition: Reactor performance, sludge morphology, and microbial community

Cited by 53 publications

References 46 publications

Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

ZVI-Mediated High-Rate Nitrogen Removal from Fulvic Acid (FA)-Containing Wastewater by Anammox: Revealing the Genomic and Molecular Level Mechanisms

Combined Phototrophic Simultaneous Nitrification-Endogenous Denitrification with Phosphorus Removal (P-SNDPR) System Treating Low Carbon to Nitrogen Ratio Wastewater for Potential Carbon Neutrality

Contact Info

Product

Resources

About