Recovery of campylobacter from human faeces stored at 4 °C

Abstract

Six hundred and thirteen fresh diarrhoeal faeces were inoculated on Skirrow blood agar (SK), on Preston blood free agar (PBF), and in Campy-thioglycolate broth (CT). After 24 h of storage at 4 degrees C, specimens were again inoculated on SK and PBF, and in Campylobacter enrichment broth (CEB). CT tubes were placed overnight at 4 degrees C. Plates and CEB tubes were incubated at 43 degrees C in microaerophilic conditions. A total of 68 specimens was positive for campylobacter on direct plating. Sixty-four of them were also recovered after subculturing from CT, and only 51 from CEB. Delayed inoculation of plates after storage of samples at 4 degrees C yielded 57 isolates. The storage of faeces at 4 degrees C for 24 h significantly reduces the number of campylobacter isolates. When samples are not plated immediately we recommend inoculating a CT tube maintained at 4 degrees C overnight as a holding medium.

Molecular-based detection of non-culturable and emerging campylobacteria in patients presenting with gastroenteritis

“…In support of this, Ladron de Guevara et al . [6] previously reported that 16·2% of Campylobacter -positive samples failed to grow when comparing immediate culture with a 24-h delay stored at 4°C; a figure which aligns closely with our finding here. We found that 85·9% of the C. jejuni-/C.…”

“…Unlike the earlier study [5] in which samples were cultured on the day of receipt, here samples were stored at 4 xC and cultured retrospectively once identified as Campylobacter-positive using the EntericBio method. In support of this, Ladron de Guevara et al [6] previously reported that 16 . 2% of Campylobacter-positive samples failed to grow when comparing immediate culture with a 24-h delay stored at 4 xC ; a figure which aligns closely with our finding here.…”

Problems in Isolation of Campylobacter jejuni from Frozen-Stored Raw Milk and Bovine Fecal Samples: Genetic Confirmation Using Multiplex PCR

“…Although we anticipated differences in the cryoprotective effects of bovine versus human fecal samples, Dan et al (1989), using human fecal samples, reported that at 4°C C. jejuni survived for Ͻ1 month, with or without cryopreservatives. Ladron de Guevara et al (1989) indicated that storage of human feces at 4°C for 24 h significantly reduced numbers of viable C. jejuni. A study by Mills and Gherna (1988) demonstrated that pure cultures of C. jejuni could not maintain viability at Ϫ20°C after storage for 7 months using glycerol as cryopreservative.…”