2012
DOI: 10.1016/j.mimet.2012.10.001
|View full text |Cite
|
Sign up to set email alerts
|

Recovery of diverse microbes in high turbidity surface water samples using dead-end ultrafiltration

Abstract: Dead-end ultrafiltration (DEUF) has been reported to be a simple, field-deployable technique for recovering bacteria, viruses, and parasites from large-volume water samples for water quality testing and waterborne disease investigations. While DEUF has been reported for application to water samples having relatively low turbidity, little information is available regarding recovery efficiencies for this technique when applied to sampling turbid water samples such as those commonly found in lakes and rivers. Thi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
86
3
3

Year Published

2013
2013
2023
2023

Publication Types

Select...
6
3
1

Relationship

3
7

Authors

Journals

citations
Cited by 92 publications
(93 citation statements)
references
References 24 publications
1
86
3
3
Order By: Relevance
“…At each site, 100 L of water was concentrated using dead-end hollow-fiber ultrafiltration (DEUF). 13,14 A 1-L grab sample was also collected to determine whether this simple, small-volume collection method could be as effective as a more labor-intensive large-volume method such as DEUF. Plankton samples (PLK) containing zooplankton and phytoplankton were collected by filtering 100 L through a 20-μm mesh plankton net fitted with a polyvinyl chloride (PVC) cod end bucket (Aquatic Research Instruments, Lehmi, ID) to obtain a final volume of 1 L. A plankton net-filtered water (PFW) fraction containing free-living bacteria and plankton 20 μm was obtained by collecting 1 L of the flow-through from the plankton net.…”
Section: Methodsmentioning
confidence: 99%
“…At each site, 100 L of water was concentrated using dead-end hollow-fiber ultrafiltration (DEUF). 13,14 A 1-L grab sample was also collected to determine whether this simple, small-volume collection method could be as effective as a more labor-intensive large-volume method such as DEUF. Plankton samples (PLK) containing zooplankton and phytoplankton were collected by filtering 100 L through a 20-μm mesh plankton net fitted with a polyvinyl chloride (PVC) cod end bucket (Aquatic Research Instruments, Lehmi, ID) to obtain a final volume of 1 L. A plankton net-filtered water (PFW) fraction containing free-living bacteria and plankton 20 μm was obtained by collecting 1 L of the flow-through from the plankton net.…”
Section: Methodsmentioning
confidence: 99%
“…This has increased the logistical feasibility of testing water sources for multiple bacteria, viruses and parasites. [13] DEUF can recover diverse microbes from large volumes of water (~100 L); [13] however, this was only the second time the method was applied to flood water, which can be highly turbid. [14] …”
Section: Introductionmentioning
confidence: 99%
“…Therefore, assessing drinking well vulnerability can involve a multiple samplings, perhaps more than might be used for traditional contaminant vulnerability assessments. Fortunately, water samples for viruses can now be collected inexpensively and routinely (Lambertini et al 2008;Gibbons et al 2010;Mull and Hill 2012), which allows affordable collecting of larger sample numbers. In the early 2000s, results from viral analysis by conventional polymerase chain reaction (PCR) usually included only virus identification and presence/absence; virus quantification could only be accomplished by culture methods and these are laborious, expensive, and restricted to only a few virus groups.…”
Section: Human-introduced Contaminant (Biological): Human Enteric Virmentioning
confidence: 99%