Recovery of functional materials with thermally stable antioxidative properties in squid muscle hydrolyzates by subcritical water

Abstract

Subcritical water hydrolysis was carried out to produce functional materials from squid muscle using a batch reactor. The reaction temperatures and pressures for hydrolysis of thermal dried squid muscle were maintained from 160 to 280°C and 6 to 66 bar for 3 min. The ratio of material to water for hydrolysis was 1:25 (w/v) and it was stirred at 140 rpm. Hydrolysis yield was increased after increasing the temperature and pressure while the protein in hydrolyzate decreased with the rise of temperature. The reducing sugar yield was high at temperature 220°C in subcritical water hydrolysis of squid muscle. Low molecular weight peptides were found in all hydrolyzates by SDS-PAGE. The highest yield of free and structural amino acid in hydrolyzate was 421.53±1.24 and 380.58±2.25 mg/100 g, respectively at 250°C. All essential amino acids were identified in muscle hydrolyzates and it was high at 220°C. Among the essential amino acids, leucine was the most abundant. Antioxidative properties were found in all hydrolyzates and it was high at 220°C. More than 98±0.26 % ABTS antioxidant activity was retained in hydrolyzates after long time heat treatment.

Recovery of Natural Antioxidants from Agro-Industrial Side Streams through Advanced Extraction Techniques

“…Asaduzzaman and Chun studied the recovery of amino acids and peptides from squid muscle, by subcritical water extraction. The optimal recovery conditions were established 250 °C for amino acids, 160 °C for peptides, and some complex amino acids were degraded at 250 °C [139]. Zhu et al recovered amino acids from poultry wastes (chicken intestine), with a proper establishment of the process parameters (reaction temperature 533 K, reaction time 28 min and H 2 SO 4 concentration in reactant system 0.02%) [140].…”

Utilization of Slaughterhouse Waste in Value-Added Applications: Recent Advances in the Development of Wood Adhesives

“…Reportedly, the amino acids are highly labile under subcritical hydrothermal conditions, and undergo further degradation resulting in short chain fatty acids [60,61,62,63]. In agreement with the further degradation of amino acids at higher hydrolysis temperatures, Asaduzzman and Chung found that the amino acid yields also decreased with increasing temperature [46]. However, it is worth mentioning that the effect of temperature and extent of hydrolysis is dependent on the protein feedstock as well as end application of the recovered hydrolyzate.…”

“…In several studies, partial hydrolysis of proteins has been found to be beneficial for formulating wood adhesives as hydrolysis promotes unfolding of protein molecules thereby exposing reactive functional groups and making them available to interact with the functional groups of the substrate [ 18 ]. A review of the available literature indicates that enzymatic treatment [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ], acid or alkaline hydrolysis [ 21 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ], and thermal hydrolysis using subcritical water [ 21 , 46 , 47 , 48 , 49 , 50 ] are commonly used methods for preparation of protein hydrolyzates from proteinaceous waste. Bacterial treatment using keratinolytic microorganisms has also been reported to be effective for hydrolysis of keratin protein [ 51 , 52 ].…”

“…To isolate essential amino acids and useful functional products from squid muscle, Asaduzzaman and Chung (2015) conducted thermohydrolysis of dried proteinaceous mass using subcritical water in the temperature range of 160 to 280 °C and a pressure of 6 to 66 bar. Reportedly, enhancement in protein hydrolysis was observed with an increase in temperature and pressure, and a nearly quantitative yield of hydrolysis was achieved at a temperature of 280 °C [ 46 ]. The likely reason for this is the effect of temperature and pressure on activation energy of the reaction.…”

Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress

“…Amino acid compositions were analyzed using an amino acid auto analyzer (S433-H, SYKAM, Eresing, Germany) as described by Asaduzzaman and Chun (2015) [ 23 ]. The samples were introduced to an LCA K06/Na cation separation column (4.6 × 150 mm) and eluted with 5 mM of p-toluenesulfonic acid solution as the mobile phase at a flow rate of 0.45 mL/min.…”