Recovery of
            <i>Salmonella</i>
            by Using Selenite Brilliant Green Sulfa Enrichment Broth

Chang, Chun‐Chi; Yuo, Chung-Yee; Shen, Hui-Ching; Li, A-Mai; Chen, Chao-yu; Chou, Jui-ling; Huang, Shih-Hui

doi:10.1128/jcm.37.12.4120-4123.1999

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…The number of Salmonella increased up to (7.3±0.2) log CFU/chicken, followed by TSB media [(7.1±0.4) log CFU/chicken]. The result of this study was in good agreement with the result of Boer(17), which demonstrated that BHI and TSB exhibited significantly greater increases than other non-selective media. However, there were no significant differences among the media until 4 hr (p<0.05).Unlike other media, the number of Salmonella enriched in SC did not increase obviously between 2 hr and 4 hr.…”

supporting

confidence: 86%

저온저장으로 인해 손상된 살모넬라를 배양하기 위한 최적의 배지 선정에 관한 연구

Park¹

2016

Korean Journal of Food Preservation

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This purpose of this study was to determine the best enrichment medium for rejuvenating and recovering Salmonella placed in cold temperature prior to the employment of the gold biosensor combined with a light microscopic imaging system. A mixture of nalidixic-resistant Salmonella Typhimurium and Enteritidis were inoculated onto chicken (1,000 CFU/chicken). After cold injury at 4℃ for 24 hr, Salmonella on chicken was enriched for 6 hr with six non-selective media including buffered peptone water broth, lactose broth, brain heart infusion broth (BHI), universal pre-enrichment broth, nutrient broth, and tryptic soy broth, and five selective media including brilliant green broth (BG), rappaport-vassiliadis R10 broth, selenite cystine broth, selenite broth, and tetrathionate brilliant green broth (TBG) for the comparison of Salmonella growth. Various concentrations of Salmonella (10, 50, 100, 500, and 1,000 CFU/chicken) were then enriched for 6 hr in both BHI and BG media to select the best media. BHI was selected as the most effective non-selective enrichment medium, while BG was selected as the most effective selective enrichment medium. Finally, BHI medium was selected as the most efficient enrichment medium for Salmonella growth injured from cold temperature during processing or storage.

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supporting

confidence: 86%

저온저장으로 인해 손상된 살모넬라를 배양하기 위한 최적의 배지 선정에 관한 연구

Park¹

2016

Korean Journal of Food Preservation

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“…Milk and soft cheese samples were added to an enrichment media and then incubated at 36±1°C for 24 h for the further investigation. To identify the pathogen, different enrichment media were used as follow: Trypticase Soy Broth (TSB) for Escherichia coli O157:H7 (Lye et al 2013); Baird-Parker Agar Base for Staphylococcus aureus (Rahimi and Alian 2013); Listeria Enrichment Broth Base for Listeria monocytogenes (Amagliani et al 2006); and selenite brilliant green sulfa enrichment (SBG) broth for Salmonella enterica serovar Typhimurium (Chang et al 1999).…”

Section: Microbial Enrichmentmentioning

confidence: 99%

PCR Detection of Pathogenic Bacteria in Milk and Soft Cheese Under Different Cow’s Production Systems in Mexico

Elfarash

Solorio‐Sánchez

Rojas-Herrera

et al. 2016

Research Journal of Applied Biotechnology

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Thirty six samples of raw milk were collected from three different farms located in Merida, Yucatan, Mexico and seventy two samples of soft cheese were manufactured from thirty six samples of raw milk and thirty six samples of pasteurized milk from the previous collected raw milk during May 2013 to April 2014. The first and second farm located at Faculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan (FMVZ/UADY) used two different production regimes; intensive silvopastoral system (ISS) and traditional system (TS). The third farm in Kampepen under (ISS). The collected raw milk samples were divided into three portions, one portion of raw milk and two portions for soft cheese manufacture from (raw and pasteurized milk) for PCR screening. PCR screening was performed for one hundred and eight samples to detect four types of the most dangerous foodborne pathogens (Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, Staphylococcus aureus and Listeria monocytogenes). PCR results showed that almost samples were negative for all tested pathogens except six samples (5.56%); 3 samples of raw milk and other 3 samples of soft cheese manufactured from this raw milk under traditional system which were positive for Staphylococcus aureus.

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“…(USDA, 2019). Hektoen Enteric (HE) agar inhibits gram‐positive bacteria and differentiates Salmonella using bile salts, bromothymol blue, and acid fuchsin dyes to produce blue to blue‐green colonies with a black center (Chang et al, 1999; Goo, Ching, & Gooch, 1973; King et al, 2005; Kristensen, Lester, & Jürgens, 1925).…”

Section: Introductionmentioning

confidence: 99%

Comparison of selective enrichment and plating media for Salmonella isolation from broiler carcasses

Obe

Berrang

Cox

et al. 2021

Journal of Food Safety

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Salmonella detection and isolation rely on different selective enrichment media, which can influence which serovars are detected. The objective of this study was to compare Salmonella recovery from broiler carcass rinses using three different selective enrichment protocols and three differential plating agars. Eight prechill broiler carcasses were collected at a commercial slaughter facility. Each carcass was subjected to whole carcass rinse procedure in buffered peptone water (BPW). An aliquot of the rinse and whole carcasses in the remaining rinse were incubated as a pre‐enrichment before subculturing in selective enrichment broths (Rappaport Vassiliadis [RV], Tetrathionate Hajna [TT], and TT to RV in series [TT‐to‐RV]). Enriched samples were streaked on the three differential agars (Hektoen Enteric [HE], Brilliant Green Sulfa [BGS], and Xylose‐Lysine‐Tergitol‐4 [XLT‐4]). Salmonella was isolated from all eight carcasses. Considering all sample preparations as independent subsamples, Salmonella was detected in 88% (128/144) of subsamples with a 100% recovery from the TT‐to‐RV enrichment, and 92 and 71% from RV and TT broths, individually. A high concordance in recovery on BGS versus XLT‐4 agar plates was observed compared to HE versus BGS and HE versus XLT‐4 plates. These data suggest that choice of pre‐enrichment method, selective enrichment medium, and differential agar can influence the recovery of Salmonella from poultry samples.

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Recovery of Salmonella by Using Selenite Brilliant Green Sulfa Enrichment Broth

Cited by 8 publications

References 13 publications

저온저장으로 인해 손상된 살모넬라를 배양하기 위한 최적의 배지 선정에 관한 연구

저온저장으로 인해 손상된 살모넬라를 배양하기 위한 최적의 배지 선정에 관한 연구

PCR Detection of Pathogenic Bacteria in Milk and Soft Cheese Under Different Cow’s Production Systems in Mexico

Comparison of selective enrichment and plating media for Salmonella isolation from broiler carcasses

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