Recovery of lactic acid from simultaneous saccharification and fermentation media using anion exchange resins

Abstract

The physicochemical properties (capacity, kinetics and selectivity) of the ion exchange resins Amberlite IRA900, IRA400, IRA96 and IRA67 were determined to evaluate their comparative suitability for lactic acid recovery. Both the kinetics of lactic acid sorption from aqueous solutions and the equilibrium were assessed using mathematical models, which provided a close interpretation of the experimental results. The best resins (Amberlite IRA96 and IRA67) were employed in further fixed-bed operation using aqueous lactic acid solutions as feed. In this set of experiments, parameters such as capacity, regenerant consumption, percentage of lactic acid recovery and product concentration were measured. Amberlite IRA67, a weak base resin, was selected for lactic acid recovery from SSF (simultaneous saccharification and fermentation) broths. Owing to the presence of nutrients and ions other than lactate, a slightly decreased capacity was determined when using SSF media instead aqueous lactic acid solutions, but quantitative lactic acid recoveries at constant capacities were obtained in four sequential load/regeneration cycles.

L(+)-Lactic acid recovery from cassava bagasse based fermented medium using anion exchange resins

“…However, comparatively high amounts of solvents are needed for extraction, and toxic effects are provoked by solvents. In the electrodialysis units, cells adhered to membranes during the process, leading to decreased efficiency in the overall recovery system (Nomura et al, 1987;Davison and Scott, 1992;Zihao and Kefeng, 1995;Moldes et al, 2003). Lactic acid from fermentation broth can be recovered using anion polymeric adsorbents, which were strong, moderate, or weak basic anion exchange resins adsorbing lactic acid below and above of its pKa (Moldes et al, 2003;Kulprathipanja and Oroshar, 1991).…”

Adsorption Behaviour of Lactic Acid on Granular Activated Carbon and Anionic Resins: Thermodynamics, Isotherms and Kinetic Studies

“…The model fermentation broth was prepared from an analytical grade 88% L-(+)lactic acid stock solution (Sigma-aldrich Cheme BV, Zwijndrecht, The Netherlands) and demineralized water as described by Moldes et al [17]. The initial pH (2.0-6.5) of the bulk solution was adjusted by either adding 6.0 M NaOH or 6.0 M HCl.…”

“…Among these techniques, adsorption by activated carbon and anionic resins are suitable for fermentation broths with low concentrations of lactic acid, especially from heterolactic fermentation [16,17]. A wide range of anionic resins are commercially available with various functional groups and support matrices, which makes the adsorption process more flexible under various operating conditions.…”

Secreted expression of Leuconostoc mesenteroides glucansucrase in Lactococcus lactis for the production of insoluble glucans

“…Lastly, attempts to increase cell density using anion exchange resins for continuous removal of lactic acid yielded mixed results. Numerous studies have described utilizing anion exchange resins for coupled fermentation and lactic acid recovery (Chen and Ju 2002;Moldes et al 2003) and for increasing nisin production by L. lactis (Tolonen et al 2004;Yu et al 2002). While we were able to significantly increase cell density using IRA 400 and IRA 96 in shake flask cultures and with IRA 400 in pH controlled bioreactors, the accumulated glucansucrase activity was always less than that obtained in pH controlled bioreactors without anion exchange resins.…”