Corn tortillas are produced using the traditional nixtamalisation process (TP). Nevertheless, one of the greatest problems that the tortilla industry faces is the high level of losses of solids. The aim of this research was to evaluate the nutritional value of a whole-grain tortilla, produced using an ecological process (EP). In the EP corn was cooked at 90°C with 1.8% (w ⁄ w) calcium sulphate. The grains were steeped, washed and grounded to obtain the masa, which was baked to produce tortillas. Also, tortillas were prepared using the TP and following the same steps as those used in the EP, except that 1.0% (w ⁄ w) of calcium hydroxide was used. The tortillas from EP retained 14.05, 6.03, and 54.02% more fat, protein and fibre than tortillas produced by the TP. The PER value of rats fed whole-grain tortillas (1.81) was not different from that of rats fed the traditional tortillas (1.85).