Recovery of paddy soil methanotrophs from long term drought

Collet, Sebastian; Reim, Andreas; Ho, Adrian; Frenzel, Peter

doi:10.1016/j.soilbio.2015.04.016

Cited by 22 publications

(19 citation statements)

References 25 publications

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“…The elevated methane uptake in response to severe disturbance in cycles two and three ( Figure 1 ) is noteworthy, but remains to be fully elucidated. Previous studies showed the remarkable resilience of methanotrophic activity to distinct physical disturbances (e.g., disturbance-induced mortality; Ho et al, 2011a , heat stress; Whittenbury et al, 1970 ; Ho and Frenzel, 2012 ; prolonged drought; Collet et al, 2015 , grinding; Kumaresan et al, 2011 ). These disturbances, however, were induced as a single event, and the response of methane uptake and the methanotrophic community composition were monitored during recovery.…”

Section: Resultsmentioning

confidence: 99%

“…Inducing disturbances as single events, aerobic methanotrophs showed remarkable resilience to prolonged drought ( Collet et al, 2015 ), heat stress ( Whittenbury et al, 1970 ; Ho and Frenzel, 2012 ), soil structural disruption ( Kumaresan et al, 2011 ), and a disturbance-induced motility, monitoring re-colonization after a simulated die-off ( Ho et al, 2011a ; Pan et al, 2014 ). Although diversity decreased after disturbance, methanotrophic activity was not adversely compromised, and was even over compensated during recovery when compared to the un-disturbed community ( Ho et al, 2011a ; Kumaresan et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Recurrence and Frequency of Disturbance have Cumulative Effect on Methanotrophic Activity, Abundance, and Community Structure

Brink

Reim

et al. 2016

Front. Microbiol.

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Alternate prolonged drought and heavy rainfall is predicted to intensify with global warming. Desiccation-rewetting events alter the soil quality and nutrient concentrations which drive microbial-mediated processes, including methane oxidation, a key biogeochemical process catalyzed by methanotrophic bacteria. Although aerobic methanotrophs showed remarkable resilience to a suite of physical disturbances induced as a single event, their resilience to recurring disturbances is less known. Here, using a rice field soil in a microcosm study, we determined whether recurrence and frequency of desiccation-rewetting impose an accumulating effect on the methanotrophic activity. The response of key aerobic methanotroph subgroups (type Ia, Ib, and II) were monitored using qPCR assays, and was supported by a t-RFLP analysis. The methanotrophic activity was resilient to recurring desiccation-rewetting, but increasing the frequency of the disturbance by twofold significantly decreased methane uptake rate. Both the qPCR and t-RFLP analyses were congruent, showing the dominance of type Ia/Ib methanotrophs prior to disturbance, and after disturbance, the recovering community was predominantly comprised of type Ia (Methylobacter) methanotrophs. Both type Ib and type II (Methylosinus/Methylocystis) methanotrophs were adversely affected by the disturbance, but type II methanotrophs showed recovery over time, indicating relatively higher resilience to the disturbance. This revealed distinct, yet unrecognized traits among the methanotroph community members. Our results show that recurring desiccation-rewetting before a recovery in community abundance had an accumulated effect, compromising methanotrophic activity. While methanotrophs may recover well following sporadic disturbances, their resilience may reach a ‘tipping point’ where activity no longer recovered if disturbance persists and increase in frequency.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recurrence and Frequency of Disturbance have Cumulative Effect on Methanotrophic Activity, Abundance, and Community Structure

Brink

Reim

et al. 2016

Front. Microbiol.

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“…These will respond with a temporal succession of species (Collet et al. , Ho et al. , b), a process that will only stabilize function through time if a sufficiently diverse pool of taxa is available.…”

Section: Discussionmentioning

confidence: 99%

Positive diversity‐functioning relationships in model communities of methanotrophic bacteria

Schnyder

Bodelier

Hartmann

et al. 2018

Ecology

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we tested effects of biodiversity on the functioning of methanotrophs, a specialized group of soil 27 bacteria that plays a key role in mediating greenhouse gas emissions from soils. Using pure 28 strains of methanotrophic bacteria, we assembled artificial communities of different diversity 29 levels, with which we inoculated sterile soil microcosms. To assess the functioning of these 30 communities, we measured methane oxidation by gas chromatography throughout the 31 experiment and determined changes in community composition and community size at several 32 time points by quantitative PCR and sequencing. We demonstrate that microbial diversity had a 33 positive overyielding-effect on methane oxidation, in particular at the beginning of the 34 experiment. This higher assimilation of CH 4 at high diversity translated into increased growth 35 and significantly larger communities towards the end of the study. The overyielding of mixtures 36 with respect to CH 4 consumption and community size was positively correlated. The temporal 37 CH 4 consumption profiles of strain monocultures differed, raising the possibility that temporal 38 complementarity of component strains drove the observed community-level strain richness 39 effects; however, the community niche metric we derived from the temporal activity profiles did 40 not explain the observed strain richness effect. The strain richness effect also was unrelated to 41 both the phylogenetic and functional trait diversity of mixed communities. Overall, our results 42 suggest that positive biodiversity-ecosystem function relationships show similar patterns across 43 different scales and may be widespread in nature. Additionally, biodiversity is probably also 44 important in natural methanotrophic communities for the ecosystem function methane oxidation. 45 3 Therefore, maintaining soil conditions that support a high diversity of methanotrophs may help 46 to reduce the emission of the greenhouse gas methane. 47 48

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“…Methanotroph diversity may buffer ecosystem functioning during environmental fluctuations [58][59][60]. Methanotrophs isolated from environmental samples typically exhibit distinct temperature optima.…”

Section: Discussionmentioning

confidence: 99%

Experimental erosion of microbial diversity decreases soil CH₄consumption rates

Schnyder

Hartmann

et al. 2020

Preprint

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Biodiversity-ecosystem functioning (BEF) experiments have predominantly focused on communities of higher organisms, in particular plants, with comparably little known to date about the relevance of biodiversity for microbiallydriven biogeochemical processes. Methanotrophic bacteria play a key role in Earth's methane (CH 4 ) cycle by removing atmospheric CH 4 and reducing emissions from methanogenesis in wetlands and landfills. Here, we used a dilution-to-extinction approach to simulate diversity loss in a methanotrophic landfill cover soil community. Combining analyses of CH 4 flux and community structure, we found a linear decrease of CH 4 oxidation rates with the number of taxonomic units lost. This effect was independent of community size, consistent over the three-month study, and occurred in relatively diverse communities, challenging the notion of high functional redundancy mediating high resistance to diversity erosion in natural microbial systems. The effects we report resemble the ones for higher organisms, suggesting that BEF-relationships are universal across taxa and spatial scales.

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Recovery of paddy soil methanotrophs from long term drought

Cited by 22 publications

References 25 publications

Recurrence and Frequency of Disturbance have Cumulative Effect on Methanotrophic Activity, Abundance, and Community Structure

Recurrence and Frequency of Disturbance have Cumulative Effect on Methanotrophic Activity, Abundance, and Community Structure

Positive diversity‐functioning relationships in model communities of methanotrophic bacteria

Experimental erosion of microbial diversity decreases soil CH₄consumption rates

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Recovery of paddy soil methanotrophs from long term drought

Cited by 22 publications

References 25 publications

Recurrence and Frequency of Disturbance have Cumulative Effect on Methanotrophic Activity, Abundance, and Community Structure

Recurrence and Frequency of Disturbance have Cumulative Effect on Methanotrophic Activity, Abundance, and Community Structure

Positive diversity‐functioning relationships in model communities of methanotrophic bacteria

Experimental erosion of microbial diversity decreases soil CH4consumption rates

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Experimental erosion of microbial diversity decreases soil CH₄consumption rates