Recovery of Proteins and their Biofunctionalities from Marine Algae

Jeon, You‐Jin; Samarakoon, Kalpa W.

doi:10.1002/9781118375082.ch12

Cited by 1 publication

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“…The active ones are typically isolated from the mixture using membrane filtration and reverse-phase chromatography techniques [ 90 , 91 ]. SP bioactive peptides range in sizes from 2 to 20 amino acids with the cut-off masses usually ranging from 5 kDa to 30 kDa [ 92 ]. Nonetheless, as the nature of SP varies for each seaweed, the resulting BAP profile will also be different depending on the hydrolysis conditions and protease of choice, as these account for different cut-off points [ 93 ].…”

Section: Hydrolysis and Peptide Productionmentioning

confidence: 99%

Seaweed Protein Hydrolysates and Bioactive Peptides: Extraction, Purification, and Applications

et al. 2021

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Seaweeds are industrially exploited for obtaining pigments, polysaccharides, or phenolic compounds with application in diverse fields. Nevertheless, their rich composition in fiber, minerals, and proteins, has pointed them as a useful source of these components. Seaweed proteins are nutritionally valuable and include several specific enzymes, glycoproteins, cell wall-attached proteins, phycobiliproteins, lectins, or peptides. Extraction of seaweed proteins requires the application of disruptive methods due to the heterogeneous cell wall composition of each macroalgae group. Hence, non-protein molecules like phenolics or polysaccharides may also be co-extracted, affecting the extraction yield. Therefore, depending on the macroalgae and target protein characteristics, the sample pretreatment, extraction and purification techniques must be carefully chosen. Traditional methods like solid–liquid or enzyme-assisted extraction (SLE or EAE) have proven successful. However, alternative techniques as ultrasound- or microwave-assisted extraction (UAE or MAE) can be more efficient. To obtain protein hydrolysates, these proteins are subjected to hydrolyzation reactions, whether with proteases or physical or chemical treatments that disrupt the proteins native folding. These hydrolysates and derived peptides are accounted for bioactive properties, like antioxidant, anti-inflammatory, antimicrobial, or antihypertensive activities, which can be applied to different sectors. In this work, current methods and challenges for protein extraction and purification from seaweeds are addressed, focusing on their potential industrial applications in the food, cosmetic, and pharmaceutical industries.

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