Recovery of Purification-Associated Reduction in Antigen-Induced Histamine Release from Rat Peritoneal Mast Cells.

Inagaki, Nobuya; Nakai, Norihiro; Kimata, Masahiro; Kawasaki, Hiromu; Nagai, Hiroichi

doi:10.1248/bpb.24.829

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Peritoneal exudates were collected and filtered through a 70 µm cell strainer, centrifuged at 1200 rpm for 5 minutes and re-suspended in 1 ml of sterile physiological saline. Mast cells were then purified by Percoll density gradient as previously described [32]. The purified mast cells were then cytocentrifuged at 350 x g for 5 minutes onto glass slides and were left to dry overnight before fixation in Carnoy’s fixative.…”

Section: Methodsmentioning

confidence: 99%

Disodium Cromoglycate Reverses Colonic Visceral Hypersensitivity and Influences Colonic Ion Transport in a Stress-Sensitive Rat Strain

et al. 2013

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The interface between psychiatry and stress-related gastrointestinal disorders (GI), such as irritable bowel syndrome (IBS), is well established, with anxiety and depression the most frequently occurring comorbid conditions. Moreover, stress-sensitive Wistar Kyoto (WKY) rats, which display anxiety- and depressive-like behaviors, exhibit GI disturbances akin to those observed in stress-related GI disorders. Additionally, there is mounting preclinical and clinical evidence implicating mast cells as significant contributors to the development of abdominal visceral pain in IBS. In this study we examined the effects of the rat connective tissue mast cell (CTMC) stabiliser, disodium cromoglycate (DSCG) on visceral hypersensitivity and colonic ion transport, and examined both colonic and peritoneal mast cells from stress-sensitive WKY rats. DSCG significantly decreased abdominal pain behaviors induced by colorectal distension in WKY animals independent of a reduction in colonic rat mast cell mediator release. We further demonstrated that mast cell-stimulated colonic ion transport was sensitive to inhibition by the mast cell stabiliser DSCG, an effect only observed in stress-sensitive rats. Moreover, CTMC-like mast cells were significantly increased in the colonic submucosa of WKY animals, and we observed a significant increase in the proportion of intermediate, or immature, peritoneal mast cells relative to control animals. Collectively our data further support a role for mast cells in the pathogenesis of stress-related GI disorders.

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Section: Methodsmentioning

confidence: 99%

Disodium Cromoglycate Reverses Colonic Visceral Hypersensitivity and Influences Colonic Ion Transport in a Stress-Sensitive Rat Strain

et al. 2013

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“…For some experiments, peritoneal mast cells (PMCs) were enriched by centrifugation using a Percoll gradient (MC purity, 60%-80%) as described. 27 Peripheral and mesenterial LNs were excised and single-cell suspensions were prepared by homogenization in PBS. …”

mentioning

confidence: 99%

TLR3-induced activation of mast cells modulates CD8+ T-cell recruitment

Orinska

Bulanova

Budagian

et al. 2005

Blood

156

162

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IntroductionThe magnitude and quality of innate immune response is essential for appropriate adaptive response. The activation of naive CD8 ϩ T cells, the main effector cells in the course of viral infections, their clonal expansion, development of effector cells, and maintenance and expansion of memory CD8 ϩ T cells after antigen reappearance are precisely regulated and dependent on an adequate context of costimulation and cytokine/chemokine environment, provided by cells responding to "first defense line" signals of innate immunity. [1][2][3][4] Mast cells (MCs), pivotal effector cells in immunoglobulin (Ig) E-associated disorders, have recently been recognized as important elements in innate immune defenses. 5 Strategically located at host/environment interfaces like the skin, airways, and gastrointestinal and urogenital tracts, 6,7 MCs are equipped with a large variety of receptors to detect signs of infections including Toll-like receptors (TLRs), CD48, and complement receptors. 8 MCs activated via these receptors secrete a large number of proinflammatory products including granule-associated preformed mediators (histamine, serotonin, proteases, proteoglycans), lipid mediators (leukotrienes B 4 and C 4 and prostaglandins), as well as cytokines, chemokines, and growth factors. 9 In contrast to the well-established crucial role of MCs in induction of host defense responses to bacteria and parasites, the function of MCs in antiviral immune responses remains to be characterized in detail. Recently, a number of reports have shown the functional interplay between MCs and T cells in allergic, infectious, and autoimmune processes, for example, MCs and T cells colocalizing in inflamed tissues, as in the lungs of patients with asthma and in animal models of allergic asthma. 10,11 It has also been reported that tumor necrosis factor ␣ (TNF-␣) released from MCs contributes substantially to T-cell recruitment to the draining lymph nodes (LNs) in an experimental infection model with Escherichia coli. 12 Furthermore, activated MCs induce the chemotaxis of CD8 ϩ T cells through the production of leukotrienes in vitro. 13 Further, the recruitment of early effector T cells into the airways in an asthma model is mediated by leukotriene B 4 (LTB 4 ) 14 released by MCs activated by IgE cross-linking. 15 However, the mechanisms and relevance of MC-T-cell interactions, as well as the signals required for MC activation and effector functions in settings of viral infections remain largely unknown.TLRs play an important role in innate immunity recognizing specific, nonself, conserved components shared by different pathogens. Different TLRs are involved in the specific recognition of various microbes. 16,17 Human and mouse MCs have been shown to be activated by bacterial peptidoglycan and lipopolysaccharide (LPS) via TLR2 and TLR4, respectively, producing a number of cytokines such as interleukin 5 (IL-5), IL-6, IL-10, IL-13, and TNF-␣. [18][19][20][21] On binding of viral dsRNA or its synthetic counterpart polyinosinic-polycytidyl...

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“…Mast cells were isolated by peritoneal lavage as previously described (Berdún et al, 2015) with some modifications (Coll et al, 2020). Peritoneal mast cells were purified by centrifugation through a discontinuous gradient of Percoll (Inagaki et al, 2001). Cells were stained with toluidine blue (0.1% (w/v), pH 1.0) and quantified using a Neubauer hemocytometer under a Nikon microscope (magnification 400×).…”

Section: Methodsmentioning

confidence: 99%

Section: Isolation and Purification Of Mast Cellsmentioning

confidence: 99%

Dehydroleucodine and xanthatin, two natural anti‐inflammatory lactones, inhibit mast cell degranulation by affecting the actin cytoskeleton

Wetten,

Arismendi Sosa,

Mariani

et al. 2023

Cytoskeleton

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Actin remodeling is a critical regulator of mast cell secretion. In previous work, we have shown that dehydroleucodine and xanthatin, two natural α,β‐unsaturated lactones, exhibit anti‐inflammatory and mast cell stabilizing properties. Based on this background, this study aimed to determine whether the mast cell stabilizing action of these lactones is associated with changes in the actin cytoskeleton. Rat peritoneal mast cells were preincubated in the presence of dehydroleucodine or xanthatin before incubation with compound 48/80. Comparative studies with sodium cromoglycate and latrunculin B were also made. After treatments, different assays were performed on mast cell samples: β‐hexosaminidase release, cell viability studies, quantification of mast cells and their state of degranulation by light microscopy, transmission electron microscopy, and actin staining for microscopy observation. Results showed that dehydroleucodine and xanthatin inhibited mast cell degranulation, evidenced by the inhibition of β‐hexosaminidase release and decreased degranulated mast cell percentage. At the same time, both lactones altered the F‐actin cytoskeleton in mast cells resulting, similarly to Latrunculin B, in a higher concentration of nuclear F‐actin when activated by compound 48/80. For the first time, this study describes the biological properties of dehydroleucodine and xanthatin concerning to the rearrangement of actin filaments during stimulated exocytosis in mast cells. These data have important implications for developing new anti‐inflammatory and mast cell stabilizing drugs and for designing new small molecules that may interact with the actin cytoskeleton.

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Recovery of Purification-Associated Reduction in Antigen-Induced Histamine Release from Rat Peritoneal Mast Cells.

Cited by 7 publications

References 29 publications

Disodium Cromoglycate Reverses Colonic Visceral Hypersensitivity and Influences Colonic Ion Transport in a Stress-Sensitive Rat Strain

Disodium Cromoglycate Reverses Colonic Visceral Hypersensitivity and Influences Colonic Ion Transport in a Stress-Sensitive Rat Strain

TLR3-induced activation of mast cells modulates CD8+ T-cell recruitment

Dehydroleucodine and xanthatin, two natural anti‐inflammatory lactones, inhibit mast cell degranulation by affecting the actin cytoskeleton

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