Recovery of soil streptomycetes from arid habitats in Jordan and their potential to inhibit multi-drug resistant Pseudomonas aeruginosa pathogens

Saadoun, Ismail; Wahiby, Layali; Ababneh, Qotaiba; Jaradat, Ziad W.; Massadeh, Muhannad I.; Al-Momani, Fouad

doi:10.1007/s11274-007-9451-2

Cited by 13 publications

(19 citation statements)

References 19 publications

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“…[16][17][18] h. Microbial isolates were tested against these five bacteria using disc diffusion, well diffusion and perpendicular cross streak methods [17,18].…”

Section: Chemicals Usedmentioning

confidence: 99%

Microorganisms as Chemical Factories for Isolation of Meta-Bolomes From Mesophilic Soil

Sharma

Menghani

2017

Int J Pharm Pharm Sci

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Objective: To investigate the micro-flora of the mesophilic soil of Rajasthan for isolation of novel compounds having antibacterial potentials. Methods:In present experiments, bacterial colonies were isolated from four different regions of Jaipur, Rajasthan and screened for antimicrobial efficacy against five selected pathogens Pseudomonas aeruginosa MTCC 7093, Staphylococcus aureus MTCC 7443, Escherichia coli MTCC 40, Klebsiella pneumoniae MTCC 530, and Bacillus subtillis MTCC 121. Antimicrobial efficacy against the selected strains was performed. The potential efficacy of the extract was also screened for gas chromatography-mass spectroscopy (GC-MS) analysis for novel metabolites screening. Further, potent bacterial strains were identified at the molecular level by 16S ribosomal deoxyribonucleic acid (DNA) sequencing method. Results:After the primary screening, 29 microbial isolates were selected for the screening of bioactivity. Results displayed zones of inhibition ranging from 5 mm till maximum 13 mm. Soil testing indicated survival conditions for microbes isolated, and biochemical tests supported the identification of screened isolates. The potentially isolated strains S-III C, S-III D and S-IV D were identified at the molecular level using 16S ribosomal DNA sequencing as Bacillus shackletonii, Bacillus thuringiensis and Bacillus subtilis subsp. inaquosorum, respectively. Conclusion:Extraction of active metabolites from soil microbiota, against five pathogenic bacteria, is far better, safe and economical method. This study will help in exploring new compounds against increasing number of resistant pathogenic strains with an aim to reduce demand of medicinal plants for extraction of effective antimicrobial compounds. Bacillus strains (S-IIIC, S-IIID and S-IVC) isolated from soil microflora possess antimicrobial activity and can be used for isolation of antibiotics at industrial levels.

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“…[16][17][18] h. Microbial isolates were tested against these five bacteria using disc diffusion, well diffusion and perpendicular cross streak methods [17,18].…”

Section: Chemicals Usedmentioning

confidence: 99%

Microorganisms as Chemical Factories for Isolation of Meta-Bolomes From Mesophilic Soil

Sharma

Menghani

2017

Int J Pharm Pharm Sci

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“…The antimicrobial activity was then tested against these pathogens using disc diffusion method (Saadoun et al, 2008). Plates were incubated at 38°C for 48 h.…”

Section: Antimicrobial Analysismentioning

confidence: 99%

“…Microbial isolates were tested against these five bacteria using disc diffusion, well diffusion and cross streak methods (Magaldi et al, 2004;Valgas et al, 2007;Saadoun et al, 2008;Balouiri et al, 2016).…”

Section: Test Pathogensmentioning

confidence: 99%

Antibiotic potentials and isolation of metabolomes from microorganisms of mesophilic soil of Rajasthan, India

Sharma¹,

Menghani²

2017

Afr. J. Microbiol. Res.

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The present of research work was focused on mesophilic soil samples from four different sites of Jaipur (The Pink City) in Rajasthan. Rajasthan is a semi arid region and famous for its sandy soil, heat and temperature which reach up to 50°C in summers. Twenty nine colonies were isolated from the soil samples on nutrient agar media and three distinct isolates were selected and characterized after analyzing coloration of colony formation and zone of inhibition around and appearance of the colony. On the basis of biochemical tests, the isolates are suspected to belong to the genera, Bacillus, Actinomycetes and Staphylococcus. Antibiotic activity of the isolates was studied against some common nosocomial infection causing pathogens and promising results were observed. Out of 29 colonies, only 3 colonies viz. S-IA, S-IIA and S-IIIC showed appreciable biological activity against the above mentioned 2 test pathogens E. coli and Pseudomonas aeruginosa. Further, inhibition zones were calculated in S-IA (IZ = 12 mm; AI = 1.5); S-II A (IZ = 42 mm; AI = 4.2) and S-III C (IZ = 12 mm; AI = 1.1).

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“…Samples were placed in plastic bags, transferred to the laboratory and stored in a refrigerator at 4°C. Each soil sample was crushed, thoroughly mixed and sieved through a 2 mm pore size mesh (Retsch, Germany) to get rid of large debris [15]. One g of sieved soil samples were placed in a crucible dish then heated in an oven (Supertek, India) at 45°C for 12 h [16].…”

Section: A Location Sampling Sample Processing and Treatmentmentioning

confidence: 99%

“…Streptomyces colonies that showed the largest clear zone (>1cm, the distance from the edge of the colony to the rim of the clear zone) were characterized morphologically and physiologically according to the International Streptomyces Project (ISP) [23] and as described by Saadoun et al [15].…”

Section: Characterization Of the Most Active Streptomyces Isolatesmentioning

confidence: 99%

Influence of Culture Conditions on Pectinase Production by Streptomyces sp. (Strain J9)

Saadoun

Dawagreh²,

Jaradat³

et al. 2013

LSMR

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Abstract-The purpose of this study was to determine the influence of growth conditions and medium composition on the pectinase enzyme production by Streptomyces sp. Production of pectinase by a Streptomyces strain (J9) was detected on pectin agar medium after 4 days of incubation at 28°C which exhibited a clear zone of 12 mm around the colony. Pectinase production was assayed by measuring the amount of reducing groups (D-galacturonic acid) liberated from the substrate in μmol/ml/min using the dinitrosalicylic acid assay method. The highest crude enzyme relative activity (>80%) was observed after 2 days of incubation at pH 6 and 35°C. Strain J9 produced higher amount of crude pectinase when pectin and peptone were used as carbon and nitrogen sources, respectively. Crude pectinase was maximally active at day 4 (1122 U/L) and day 5 (923 U/L) when apple and orange pomaces were used as a sole carbon source, respectively. The findings of this investigation form an avenue for production of pectinase enzyme from Streptomyces strain (J9) to be used for commercial fruit clarification and for production of transgenic plants with extended shelf life as pectinase genes cloned from this active strain.

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Recovery of soil streptomycetes from arid habitats in Jordan and their potential to inhibit multi-drug resistant Pseudomonas aeruginosa pathogens

Cited by 13 publications

References 19 publications

Microorganisms as Chemical Factories for Isolation of Meta-Bolomes From Mesophilic Soil

Microorganisms as Chemical Factories for Isolation of Meta-Bolomes From Mesophilic Soil

Antibiotic potentials and isolation of metabolomes from microorganisms of mesophilic soil of Rajasthan, India

Influence of Culture Conditions on Pectinase Production by Streptomyces sp. (Strain J9)

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