Recovery of the temperature-sensitive mutant of rous sarcoma virus from chicken cells exposed to DNA extracted from hamster cells transformed by the mutant

Hill, M.; Hillova, Jana

doi:10.1016/s0042-6822(72)80034-5

Cited by 61 publications

(18 citation statements)

References 13 publications

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“…This type of information transfer has previously been regarded as a unique property of RNA viruses presently classified as "leukoviruses" (4,8). The viral progeny arising from our DNA transfection experiments possess both the antigenic specificity and syncytial plaque characteristics as well as other markers of the original infecting RS virus used to establish this cell line, thus excluding the chance activation of a latent, unrelated adventitious agent as the principal event observed.…”

Section: Discussionmentioning

confidence: 82%

Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus.

Simpson¹,

Iinuma²

1975

Proc. Natl. Acad. Sci. U.S.A.

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The DNA fraction from a line of bovine embryonic kidney cells originally exposed as primary cultures several months earlier to a temperature-sensitive (ts) mutant of respiratory syncytial (RS) virus could be used to transfect human HEp-2 cells with the production of infectious RS virus. The DNA donor cells, designated BEK/RS ts, retained their healthy fibroblastic appearance during continuous cultivation at a temperature (390) restrictive for growth of the original infecting mutant and showed no evidence for RS virus replication or viral antigen synthesis when directly examined for these activities by conventional methods.

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Section: Discussionmentioning

confidence: 82%

Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus.

Simpson¹,

Iinuma²

1975

Proc. Natl. Acad. Sci. U.S.A.

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“…In 1972, transforming activity was reported in cellular DNA fragments transferred into other cells. The DNA had been extracted from hamster cells transformed by the ts mutant of Rous sarcoma virus (RSV) [20]. This method was successfully applied in Weinberg's laboratory [21], followed by Cooper's, Wigler's, and Barbacid's laboratories, for mouse and human cancer cell DNA fragments that transformed “normal” mouse NIH3T3 [22–25]…”

Section: Introductionmentioning

confidence: 99%

Kirsten Ras* oncogene: Significance of its discovery in human cancer research

Tsuchida

Murugan

Grieco³

2016

Oncotarget

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The KRAS/ K-RAS oncogene is crucially involved in human cancer. The term “oncogene” – i.e., a gene able to transform a normal cell into a tumor cell – was introduced in 1969, but the word was not used in the human carcinogenesis literature until much later. Transforming Kras and Hras oncogenes from the Kirsten and Harvey sarcoma viruses were not identified until the early 1980s due to the complicated structures of the viral genomes. Orthologs of these viral oncogenes were then found in transforming DNA fragments in human cancers in the form of mutated versions of the HRAS and KRAS proto-oncogenes. Thus, RAS genes were the first human oncogenes to be identified. Subsequent studies showed that mutated KRAS acted as an in vivo oncogenic driver, as indicated by studies of anti-EGFR therapy for metastatic colorectal cancers. This review addresses the historical background and experimental studies that led to the discovery ofKirsten Ras as an oncogene, the role of mutated KRAS in human carcinogenesis, and recent therapeutic studies of cancer cells with KRAS mutations.

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“…Cells infected with RNA tumor viruses contain virus-specific information in the form of DNA in their genome (1); transcription of this (proviral) DNA is thought to be an obligatory step in viral replication. Since progeny RNA is probably synthesized by host nucleoplasmic RNA polymerase (2,3) and viral RNA in transcript products can be detected with complementary probe DNA, these cells offer a system for studying the expression of specific genes in eukaryotes.…”

mentioning

confidence: 99%

Chromatin as a template for RNA synthesis in vitro.

Groner¹,

Monroy²,

Jacquet³

et al. 1975

Proc. Natl. Acad. Sci. U.S.A.

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RNA transcribed in vitro from myeloblast chromatin by exogenously added RNA polymerase B predominantly consists of short chains that remain in hybrid structure with the template; the remainder of the product is free RNA of heterogeneous size. Addition of polyanions during synthesis caused an increase in the size and amount of free RNA with a concomitant decrease in the proportion of small RNA. The large molecular weight RNA is derived from the short RNA chains, which are synthesized de novo during the reaction in vitro. The effect of polyanions on the size and nature of the product may be related to structural changes induced in the template rather than to an inhibition of nuclease activity.Cells infected with RNA tumor viruses contain virus-specific information in the form of DNA in their genome (1); transcription of this (proviral) DNA is thought to be an obligatory step in viral replication. Since progeny RNA is probably synthesized by host nucleoplasmic RNA polymerase (2, 3) and viral RNA in transcript products can be detected with complementary probe DNA, these cells offer a system for studying the expression of specific genes in eukaryotes. Recently, we reported the presence of virus-specific RNA in products synthesized in vitro from myeloblast chromatin by exogenously added eukaryotic RNA polymerase B (2). This chromatin was isolated from myeloblasts of chickens infected with avian myeloblastosis virus (AMV). The proportion of virus-specific sequences in transcript products (1-2%) was approximately the same as that in isolated infected cells (4) and that synthesized in isolated myeloblast nuclei (2). Since the amount of viral RNA in the product was 102-to 103-fold higher than expected from random transcription, it appeared that the RNA synthesizing system in vitro maintained some of the specificity exhibited during transcription in vivo. For

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Recovery of the temperature-sensitive mutant of rous sarcoma virus from chicken cells exposed to DNA extracted from hamster cells transformed by the mutant

Cited by 61 publications

References 13 publications

Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus.

Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus.

Kirsten Ras* oncogene: Significance of its discovery in human cancer research

Chromatin as a template for RNA synthesis in vitro.

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