Recovery of Totipotent Cells and Plantlet Production from Daylily Protoplasts

Fitter, Mindy S.; Krikorian, A. D.

doi:10.1093/oxfordjournals.aob.a086167

Cited by 25 publications

(9 citation statements)

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“…As far as monocots are concerned, Hemerocallis cv Autumn Blaze, the daylily, remains the only example where plants could be recovered from protoplasts (Fitter and Krikorian, 1981). Interestingly, in this case, the use of a rapidly growing, morphogenetically competent cell suspension culture as the protoplast source was the key to success, in common with all cereals and grasses (Section 7.5.3).…”

Section: Ornamentalsmentioning

confidence: 99%

Plant Regeneration from Cultured Protoplasts of Higher Plants

Ochatt

Power²,

Fowler³

et al. 1992

Plant Biotechnology

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Section: Ornamentalsmentioning

confidence: 99%

Plant Regeneration from Cultured Protoplasts of Higher Plants

Ochatt

Power²,

Fowler³

et al. 1992

Plant Biotechnology

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“…It has even been possible to make liquid suspension cultures of undifferentiated Hemerocallis cells and subsequently regenerate new plants from such material (Krikorian and Kann 1981), with the formation of embryogenic callus (Smith and Krikorian 1991). As might be expected, the amenability to tissue culture has assisted the development of systems for generating protoplasts and subsequent plant regeneration (Fitter and Krikorian 1981) and, most critically, transformation by particle bombardment (Aziz et al 2003). Transformation is exceptionally important as it permits reverse genetics, for example using specific gene silencing ‘knockdown’ technology such as hairpin RNAi and artificial microRNA.…”

Section: Practicalities As a Model Plantmentioning

confidence: 99%

A new day dawning: Hemerocallis (daylily) as a future model organism

Rodriguez-Enriquez

Grant-Downton

2012

AoB Plants

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In this point of view paper, we argue that the monocot genus Hemerocallis (daylily) satisfies multiple criteria for selection as a ‘new model organism’ for intensive biological investigation. We discuss its important and interesting attributes at the biological, horticultural and medicinal levels. These include an intriguing self-incompatibility system, a sophisticated mechanism for flower bud opening and programmed floral death, and a long history of use by man as a vegetable, ornamental and medicinal plant. We examine the potential for modern technical developments to transform Hemerocallis into a valuable model plant.

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“…Protoplasts seem to be obtainable most easily from leaf cells, but different organs and even cultured cells and tissues may be used. The latter have, in my laboratory, for reasons that are not yet completely clear, generally been a much better source of vigorous, viable protoplasts capable of sustained division (Fitter & Krikorian, 1981). Nevertheless, there is ample evidence that regeneration of wall can occur sufficiently often from protoplasts from any source to permit study (Vasil, 1976) and this is sometimes followed by cell division.…”

Section: ( 3 ) Cell or Suspension Culturementioning

confidence: 99%

“…3). In my laboratory tetraploid day lily plants have been produced through the spontaneous and random fusion of protoplasts from a diploid cultivar (Fitter & Krikorian, 1981). New tetraploids are initially produced by breeders from selected diploids through the use of colchicine but the plants so formed are chimaeras.…”

Section: )mentioning

confidence: 99%

Cloning Higher Plants From Aseptically Cultured Tissues and Cells

Krikorian

1982

Biological Reviews

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Summary 1. For a number of years considerable attention has been given both in the scientific and lay press to dramatic advances in the rapid clonal multiplication of plants by means of so‐called ‘modern’ tissue culture techniques. Because the words clone, cloning and tissue culture mean different things to different people, there is considerable confusion even among biologists as to what has actually been achieved. 2. An historical approach has been used to put the accomplishments into perspective. 3. Organ culture, tissue culture, cell or suspension culture and protoplast culture all offer varying degrees of potential for clonal multiplication but the most substantial progress has been made with explanted stem tips or lateral buds which can be stimulated to produce numerous precocious axillary branches. These, in turn, can be separated or subdivided and induced to root and thus yield populations of genetically and phenotypically uniform plantlets. 4. Similarly, undifferentiated calluses can sometimes be induced to form shoots and/or roots adventitiously. This method of stimulating organ development on callus is based on the now classic work of Skoog and Miller done in the I950s with the Wisconsin 38 strain of a ‘Havana’ cultivar of tobacco. Because of the growing criticism that when callus cultures are maintained for more than a few subcultures genetic or chromosomal aberrations are induced and these nullify the ultimate goal ‐ namely to get large numbers of genetically identical plants ‐ callus procedures are generally being relinquished in favour of precocious axillary branching methods. 5. The number of plantlets obtainable through the procedures with precocious axillary shoots or callus culture are fewer, in theory, than the number of plantlets obtainable from a somatic embryo system starting with cells grown in suspension culture. But the fact is that cell culture techniques required to produce somatic embryos are also demanding and very much in their infancy as far as their applicability to clonal multiplication is concerned. 6. Even so, slowly but steadily, advances are being made in learning how to stimulate formation of somatic or adventive embryos from totipotent cells grown in suspension culture. In cases where it has proven feasible, not only may somatic embryos be generated in great quantities, but their discrete bipolar nature often obviates the need for time‐consuming, laborious procedures to assure their success in a conventional soil or greenhouse setting. 7. While protoplast cultures are of very limited direct use for clonal propagation of higher plants, the procedures hold some hope as a vehicle whereby select useful genes can be introduced into plants. Parasexual hybridization, that is the production of hybrids by fusing totipotent protoplasts of different origin, offers no imminent prospects for the development of useful and novel plant types but may be applicable in the long term in very specific situations. 8. It is concluded that many problems exist in the producing and growing of totipo...

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Recovery of Totipotent Cells and Plantlet Production from Daylily Protoplasts

Cited by 25 publications

References 0 publications

Plant Regeneration from Cultured Protoplasts of Higher Plants

Plant Regeneration from Cultured Protoplasts of Higher Plants

A new day dawning: Hemerocallis (daylily) as a future model organism

Cloning Higher Plants From Aseptically Cultured Tissues and Cells

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