Recovery of white blood cells and platelets from leukoreduction system chambers of Trima Accel and COBE Spectra plateletpheresis devices

Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells

“…The obtained PBMC yield was in agreement with previous reports that used the Trima Accel device (Néron et al, 2007;Strasser et al, 2007).…”

Quantity, quality, and functionality of peripheral blood cells derived from residual blood of different apheresis kits

“…Research with human material such as PBMCs and immune cells strongly depends on the availability of PBMC sources, which can be a limiting factor, especially if large quantities are required. Whole blood, WBC depletion filters, buffy coats, and LRSCs from the Trima Accel plateletpheresis device are commonly used starting material for PBMC isolation and further usage of different subpopulations . The PBMC source for research purposes is mostly determined by the local blood donation service based on the blood collection systems established in their daily routine.…”

“…Those include residual cells from preparative hemapheresis devices for platelet collection such as the Trima Accel device (Terumo BCT) or the AMICUS separator system (Fenwal). It is reported that PBMCs can be gained from the leukoreduction system chamber (LRSC) of the Trima Accel device . The AMICUS separator is a widely used centrifugal separation method using plasma recirculation and elutriation .…”

Reply

“…We recently described apheresis LRS chambers as an efficient source of research‐grade PBMNCs 1 . Strasser and colleagues 2 could not replicate our data and challenged their reproducibility. They are correct that the type and settings of the apheresis machine can influence the number of recovered cells.…”

“…The reported differences between the results of Strasser and colleagues and ours with the Trima Accel are most likely associated with differences in the volume of processed blood. Strasser and colleagues processed a mean of 3.21 L during 54‐minute platelet (PLT) collections 2 . In contrast, we routinely process a mean of 4.25 L in 80 minutes in plateletpheresis collections (not including triple‐PLT‐product collections, which were not performed with LRS chambers in our report).…”