Recovery of yeast from cultivation medium by continuous flotation and its dependence on cultivation conditions

Bahr, K.H.; Schügerl, K.

doi:10.1016/0009-2509(92)80195-i

Cited by 25 publications

(4 citation statements)

References 33 publications

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“…In these applications, aqueous two-phase systems (Andrews et al, 1995;Asenjo et al, 1991;Guereca et al, 1994;HeywoodWaddington et al, 1986;Kula, 1986Kula, , 1993Walker and Lyddiatt, 1999) and other liquid-liquid systems (Borbas et al, 2001;Dennison and Lovrien, 1997;Hoeben et al, 2004;Jauregi et al, 2001Jauregi et al, , 2002Kiss et al, 1998;Pike and Denisson, 1989;Tan and Lovrien, 1972) were used to capture the desired products in the interface between the two liquids. In addition, air flotation which makes use of the adsorption of particles and/or molecules to air bubbles that rise in the liquid phase due to buoyancy, has been applied for the recovery of whole cells (Bahr and Schügerl, 1992;De Dousa et al, 2003;Gahr and Schügerl, 1992;Gaudin, 1975;Wang, 1966, 1967;Kalyuzhnyi et al, 1965;Palmieri et al, 1996;Sadowski and Golab, 1991;Tybussek et al, 1994;Vlaski et al, 1996;Wang et al, 1994). In all of these examples, interfaces are used to capture the product, but this does not necessarily mean that separation is accomplished solely with the interfacial tension force.…”

Section: Interfacial Tension Forcementioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

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The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocatalysts or particulate by-products, the recovery of product particles is a complex process. In most cases, the particulate product is purified using selective solubilization or extraction. However, if selective particle recovery is possible, the already high purity of the particles makes this downstream process more favorable. This work gives an overview of typical bioparticle mixtures that are encountered in industrial biotechnology and the various driving forces that may be used for particle-particle separation, such as the centrifugal force, the magnetic force, the electric force, and forces related to interfaces. By coupling these driving forces to the resisting forces, the limitations of using these driving forces with respect to particle size are calculated. It shows that centrifugation is not a general solution for particle-particle separation in biotechnology because the particle sizes of product and contaminating particles are often very small, thus, causing their settling velocities to be too low for efficient separation by centrifugation. Examples of such separation problems are the recovery of IBs or virus-like particles (VLPs) from (microbial) cell debris. In these cases, separation processes that use electrical forces or fluid-fluid interfaces show to have a large potential for particle-particle separation. These methods are not yet commonly applied for large-scale particle-particle separation in biotechnology and more research is required on the separation techniques and on particle characterization to facilitate successful application of these methods in industry.

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Section: Interfacial Tension Forcementioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

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“…Flotation has been examined for the recovery of S. cerevisiae (Tybussek et al, 1994), recovery of coal after microbial desulfurization (Ohmura and Saiki, 1994), foam fractionation and separation of proteins (Prokop and Tanner, 1993), recovery of yeast from cultivation medium (Bahr and Schuegerl, 1992), algae removal (Edzwald, 1993) and virus removal (Guy et al, 1976). The separation of microorganisms by flotation, in the presence of certain toxic metal ions, has been recently examined (Smith et al, 1991a, b;Sadowski et al, 1991;Yang et al, 1991).…”

Section: Flotation Of Biological Sorbentsmentioning

confidence: 98%

Removal of metal ions from dilute solutions by sorptive flotation

Zouboulis

Matis

1997

Critical Reviews in Environmental Science and Technology

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The removal of soluble ionic species, such as toxic metal cations or oxyanions, from dilute aqueous solutions, as most waste waters are, was investigated in laboratory-scale experiments (batch and continuous mode) by applying the sorptive flotation process. This method involves the preliminary abstraction or scavenging of metal ions using proper "sorbents", which exist at the fine or ultrafine particle-size range, followed by a subsequent flotation stage for the separation of metalloaded sorbent particles from the treated (cleaned) solution. The main flotation techniques for the generation of the necessary bubbles (dissolved air, dispersed, and electrolytic) were applied effectively. The examined sorbents for the initial stage of the process could be of either inorganic nature, conventional (powdered activated carbon and zeolites) or industrial solid byproducts originated in the mineral industry, or of a biological nature (biosorbents), such as those produced during fermentations, in sewage treatment plants (sludge), etc. As a result of the process application, purified water is produced (underflow) as well as a foam concentrate; the recovery and recycling of removed species is also possible from the latter, leading to an overall clean technology. If required, the sorbents may also be reused and recycled after the application of a suitable metal eluant, which can also be considered to reveal to a certain extent the disposal problem of solid wastes.

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“…The main aim of this work is to investigate whether it is possible to reach a suitable purification of water from microorganisms using a chemical free froth flotation. The driving force that leads this process is hydrophobicity and most of the microorganisms in water are hydrophobic and should be suitable for removal by such a technique (Boyles and Lincoln, 1958;Rubin et al, 1966;Bahr and Schugerl, 1992;Rios and Franca, 1997).…”

Section: Continuous Purification Processmentioning

confidence: 99%

Bio-purification of drinking water by froth flotation

Hassan

Edyvean

2019

Preprint

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<p><strong>Abstract.</strong> The main technique for removing bacteria from water for various applications is chemical disinfection. However, this method has many disadvantages such as producing disinfectant by-products (DBPs), biofilm formation and either rendering the water unpotable (at high residual disinfection) or leaving a potential for lethal diseases such as Cholera (if the residual disinfection is too low). Recently, a process was developed for continuous removal of bacteria from water using the principle of froth flotation through compressed air only without any chemicals (Hassan, 2015). This work examines the extent to which chemical free froth flotation can purify drinking water. The experiments were carried out using two flotation columns with different column lengths, each equipped with ceramic air sparger. Raw water containing bacteria was fed into the column from the top. Air was pumped through the water enough to produce a froth which separated the bacteria and, when removed, the bacterial content measured. The results show that the bacterial concentration can be reduced by 55% of its original concentration under the optimal experimental conditions so far found. This suggests that the technique can be used as a pre-purification step to minimize the use of disinfectants; hence their byproducts, and to control biofilm growth.</p>

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Recovery of yeast from cultivation medium by continuous flotation and its dependence on cultivation conditions

Cited by 25 publications

References 33 publications

Strategy for selection of methods for separation of bioparticles from particle mixtures

Strategy for selection of methods for separation of bioparticles from particle mixtures

Removal of metal ions from dilute solutions by sorptive flotation

Bio-purification of drinking water by froth flotation

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