Recreating the perivascular niche ex vivo using a microfluidic approach

Carrion, Bita; Huang, Carlos P.; Ghajar, Cyrus M.; Kachgal, Suraj; Kniazeva, Ekaterina; Jeon, Noo Li; Putnam, Andrew J.

doi:10.1002/bit.22891

Cited by 89 publications

(100 citation statements)

References 32 publications

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“…This is in line with the previous notions that a hallmark of mammalian niches is their proximity to the vascular system [36]. Blood vessels are important not only for oxygenation and nutrition of niche elements but also for providing systemic communications of stem cells.…”

Section: Discussionsupporting

confidence: 91%

Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties

Ahmadbeigi

Soleimani

Vasei

et al. 2013

Stem Cells and Development

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Although the unique role of hematopoietic stem cell (HSC) niche in hematopoiesis has long been recognized, unsuccessful isolation of intact niche units limited their in vitro study, manipulation, and therapeutic application. Here, we isolated cell complexes based on size fractionation from mouse bone marrow (BM), characterized the derived cells, and transplanted them to irradiated mice. These cell complexes were the origin of both BM mesenchymal stem cells and various hematopoietic lineages when kept in appropriate culture conditions. They also had the potential of recruiting circulating HSC. Intraperitoneal transplantation of these structures into irradiated mice not only showed long-lasting hematopoietic multilineage reconstitution, but also could recover the stromal cells of BM. In conclusion, this study for the first time provides evidences on the feasibility and efficacy of transplantation of HSC in association with their native specialized microenvironment. As the molecular cross-talk between HSC and niche is crucial for their proper function, the proposed method could be considered as a novel hematopoietic transplantation strategy.

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Section: Discussionsupporting

confidence: 91%

Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties

Ahmadbeigi

Soleimani

Vasei

et al. 2013

Stem Cells and Development

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“…It has been demonstrated that hemispherical structures with distributions of cells similar to that in the hematon can be made using positive dielectrophoresis [110]. In an attempt to recreate the perivascular niche using a microfluidic approach, Carrion and colleagues [111] suspended endothelial cells within 3D fibrin gels patterned adjacent to stromal cells (either fibroblasts or bone marrow-derived MSCs). They executed a morphogenetic process akin to vasculogenesis, forming a primitive vascular plexus and maturing it into a robust capillary network with hollow, well-defined lumens.…”

Section: Miniaturized 25 -3d-platformsmentioning

confidence: 99%

“…Both MSCs and fibroblasts formed pericytic associations with the ECs but promoted capillary morphogenesis with distinct kinetics. Biochemical assays within the niche revealed that the perivascular association of MSCs required interaction between their α6β1-integrin receptor and EC-deposited laminin [111].…”

Section: Miniaturized 25 -3d-platformsmentioning

confidence: 99%

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Gottwald¹

2017

ATROA

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Hematopoietic stem cell niches are perhaps the best described niche system in mammals. The niches themselves, as well as their cellular and structural constituents and factors that play a role in maintaining the niche structure and function, are being refined on a more or less daily basis. Despite this, it seems as if the more we know the harder it gets to mimic the in vivo-situation using in vitro-systems. This is due to the fact that hematopoiesis is a multi step maturation process leading to HSC heterogeneity. Subpopulations of HSCs and niche supporting cells can be defined depending on characteristics such as their potency of leading to successful reconstitution of sub lethally irradiated mice in serial transplantation experiments or, with less scientific impact, clonogenic assays. Since the bone marrow obviously provides all necessary information to maintain the stem cell pool constant and to adapt the number of blood cells according to physiologic needs, it has been the goal to engineer artificial niches that display at least one or several of the major characteristics of the in vivosituation to make use of these systems for not only fundamental research purposes but, moreover, also for clinical applications. This review will give an overview of approaches to engineering artificial hematopoietic niches with a focus on the complexity/dimensionality of the systems used.

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“…The challenge to create functional artificial tissues is the 3-D patterning of manifold types of cells into a tissue-like structural design. The control of components of 3-D co-culture spatial systems by microfluidics is valuable [342][343][344]. An approach using a hydrogel bi-layer of hepatocytes and endothelial cells has allowed the imitation of interstitial flow for the formation of tissue-like structures by the hepatocytes [345].…”

Section: Tissue Engineering Models On Microfluidic Chipsmentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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This paper reviews microfluidic technologies with emphasis on applications in the fields of pharmacy, biology, and tissue engineering. Design and fabrication of microfluidic systems are discussed with respect to specific biological concerns, such as biocompatibility and cell viability. Recent applications and developments on genetic analysis, cell culture, cell manipulation, biosensors, pathogen detection systems, diagnostic devices, high-throughput screening and biomaterial synthesis for tissue engineering are presented. The pros and cons of materials like polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polystyrene (PS), polycarbonate (PC), cyclic olefin copolymer (COC), glass, and silicon are discussed in terms of biocompatibility and fabrication aspects. Microfluidic devices are widely used in life sciences. Here, commercialization and research trends of microfluidics as new, easy to use, and cost-effective measurement tools at the cell/tissue level are critically reviewed.

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Recreating the perivascular niche ex vivo using a microfluidic approach

Cited by 89 publications

References 32 publications

Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties

Isolation, Characterization, and Transplantation of Bone Marrow-Derived Cell Components with Hematopoietic Stem Cell Niche Properties

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

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