Recruitment of the 40S ribosomal subunit by the West Nile virus 3′ UTR promotes the cross-talk between the viral genomic ends for translation regulation

Ramos-Lorente, Sara Esther; Berzal-Herranz, Beatriz; Romero-López, Cristina; Berzal-Herranz, Alfredo

doi:10.1016/j.virusres.2024.199340

Cited by 4 publications

(1 citation statement)

References 102 publications

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“…The 5 ′ UTR is about 100 nucleotides long and contains elements with significant roles in replication and translation [8,9]. The 3 ′ UTR is relatively larger, reaching approximately 630 nucleotides [10]. It is divided into three highly structured domains.…”

Section: Introductionmentioning

confidence: 99%

West Nile Virus Subgenomic RNAs Modulate Gene Expression in a Neuronal Cell Line

Bampali,

Kouvela,

Kesesidis

et al. 2024

Viruses

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Subgenomic flaviviral RNAs (sfRNAs) are small non-coding products of the incomplete degradation of viral genomic RNA. They accumulate during flaviviral infection and have been associated with many functional roles inside the host cell. Studies so far have demonstrated that sfRNA plays a crucial role in determining West Nile virus (WNV) pathogenicity. However, its modulatory role on neuronal homeostasis has not been studied in depth. In this study, we investigated the mechanism of sfRNA biosynthesis and its importance for WNV replication in neuronal cells. We found that sfRNA1 is functionally redundant for both replication and translation of WNV. However, the concurrent absence of sfRNA1 and sfRNA2 species is detrimental for the survival of the virus. Differential expression analysis on RNA-seq data from WT and ΔsfRNA replicon cell lines revealed transcriptional changes induced by sfRNA and identified a number of putative targets. Overall, it was shown that sfRNA contributes to the viral evasion by suppressing the interferon-mediated antiviral response. An additional differential expression analysis among replicon and control Neuro2A cells also clarified the transcriptional changes that support WNV replication in neuronal cells. Increased levels of translation and oxidative phosphorylation, post-translational modification processes, and activated DNA repair pathways were observed in replicon cell lines, while developmental processes such as axonal growth were deficient.

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Section: Introductionmentioning

confidence: 99%

West Nile Virus Subgenomic RNAs Modulate Gene Expression in a Neuronal Cell Line

Bampali,

Kouvela,

Kesesidis

et al. 2024

Viruses

View full text Add to dashboard Cite

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Structure of Essential RNA Regulatory Elements in the West Nile Virus 3′-Terminal Stem Loop

Zhu,

Chaubey,

Olsen

et al. 2024

Journal of Molecular Biology

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Decoding the genome of SARS-CoV-2: a pathway to drug development through translation inhibition

Wu,

Xiao,

Chen

et al. 2024

RNA Biology

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the coronavirus disease 2019 (COVID-19) pandemic and is continuously spreading globally. The continuous emergence of new SARS-CoV-2 variants keeps posing threats, highlighting the need for fast-acting, mutation-resistant broad-spectrum therapeutics. Protein translation is vital for SARS-CoV-2 replication, producing early non-structural proteins for RNA replication and transcription, and late structural proteins for virion assembly. Targeted blocking of viral protein translation is thus a potential approach to developing effective anti-SARS-CoV-2 drugs. SARS-CoV-2, as an obligate parasite, utilizes the host’s translation machinery. Translation-blocking strategies that target the SARS-CoV-2 mRNA, especially those that target its conserved elements are generally preferred. In this review, we discuss the current understanding of SARS-CoV-2 translation, highlighting the important conserved motifs and structures involved in its regulation. We also discuss the current strategies for blocking SARS-CoV-2 translation through viral RNA degradation or RNA element dysfunction.

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Recruitment of the 40S ribosomal subunit by the West Nile virus 3′ UTR promotes the cross-talk between the viral genomic ends for translation regulation

Cited by 4 publications

References 102 publications

West Nile Virus Subgenomic RNAs Modulate Gene Expression in a Neuronal Cell Line

West Nile Virus Subgenomic RNAs Modulate Gene Expression in a Neuronal Cell Line

Structure of Essential RNA Regulatory Elements in the West Nile Virus 3′-Terminal Stem Loop

Decoding the genome of SARS-CoV-2: a pathway to drug development through translation inhibition

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