Rectal pre-treatment with ozonized oxygen (O3) aggravates clinic status in septic rats treated with amoxicillin/clavulanate

Martin‐Barrasa, José Luis; Cordovez, Charlín Méndez; Zayas, Antonio Espinosa de los Monteros y; Ana, Ma Candelaria Juste de Santa; Clavo, Bernardino; Thomas, Pedro Herráez; Benítez, Ana Bordes; Montoya-Alonso, José Alberto; Bello, Miguel Ángel García; Artiles, Fernando; Tejedor-Junco, María Teresa

doi:10.1016/j.eimc.2014.09.006

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…At present, despite antibiotic treatment and many supporting therapies, sepsis still has a high mortality rate. Therefore, it retains its clinical and financial significance (Staatz et al 2002;Martin-Barrasa et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The body's first defence system against pathogens is the innate immune system. This system consists of cells, cytokines and mediators (van Westerloo et al 2005;Martin-Barrasa et al 2015). LPSs secreted from the bacterial wall activate many intracellular signal pathways such as nuclear Factor KB (NF-K B) and activates the body's defence mechanism by commencing inflammatory cytokine secretion (Victor et al 2004;Vaillant et al 2013;Xing et al 2015).…”

Section: Discussionmentioning

confidence: 99%

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Effects of antibiotic and intra-peritoneal ozone administration on proinflammatory cytokine formation, antioxidant levels and abdominal organ functions in the treatment of experimentally generated infectious peritonitis in rabbits

Güzel¹,

Gülçubuk²,

Yıldar³

et al. 2019

Vet. Med. - Czech

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In this study we investigated the effects of antibiotic and intraperitoneal ozone administration on pro-inflammatory cytokine, antioxidant levels and tissue damage in the treatment of experimentally infectious peritonitis. Thirty-three adult male New Zealand White Rabbits were used. The study consisted of four groups including the non-treatment group (G1), antibiotic group (G2), ozone group (G3) and ozone + antibiotic group (G4). Trimethoprim sulfadimethylprymidine was administered at a dose of 50 mg/kg subcutaneously (s.c.) and amoxicillin sodium at a dose of 15 mg/kg intramuscularly (i.m.). Medical ozone was administered intraperitoneally (i.p.) at a concentration of 30 µg O3/ml and dose of 80 ml/kg. Once peritonitis was produced, blood samples were taken from the animals before treatment and at regular intervals following treatment. Blood samples were used for haemograms and to measure levels of antioxidant and oxidative enzymes and pro-inflammatory cytokine levels. Tissue samples were examined histopathologically. There was no statistically significant difference between groups with respect to levels of pro-inflammatory cytokines. Antioxidant enzymes were found to be higher in groups G2 and G3. The granulocyte and lymphocyte values in group G3 were determined to increase earlier than in the other groups. The peritonitis scores were similar in G1 and G3, which is higher compared to G2 and G4 groups. Minimal tissue damage was observed in the group G2. It was concluded that antibiotic use for preventing peritoneum damage in experimental acute peritonitis was more effective than ozone therapy alone.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of antibiotic and intra-peritoneal ozone administration on proinflammatory cytokine formation, antioxidant levels and abdominal organ functions in the treatment of experimentally generated infectious peritonitis in rabbits

Güzel¹,

Gülçubuk²,

Yıldar³

et al. 2019

Vet. Med. - Czech

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“…In a septic rat model study by Torossian et al 17 ozone pre-treatment seemed to reduce the survival rates. Martin-Barrasa et al 18 reported that rats which ozone was applied as an adjunct to antibiotic treatment had a worsening of clinical condition compared to animals which treated only with antibiotic in a peritoneal sepsis model.…”

Section: ■ Discussionmentioning

confidence: 99%

The effects of ozone on bacterial growth and thiol-disulphide homeostasis in vascular graft infection caused by MRSA in rats

Öztürk¹,

Kurtoğlu

Durmaz

et al. 2017

Acta Cir. Bras.

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“…In all lungs, the presence of oedemas around vessels was evaluated. In addition to this, lung injury was determined based on different signs, such as oedema, haemorrhage, leukocyte infiltration and alveolar septal thickening and pleuritis (Martín- Barrasa et al, 2015).…”

Section: Rna Extraction and Real Time Pcr (Rt-qpcr)mentioning

confidence: 99%

An aqueous pomegranate peel extract (Punica granatum) protect against Elastase-induced pulmonary emphysema in Sprague Dawley rats model

Zioud

Luis

Antonio

et al. 2021

Braz. J. Pharm. Sci.

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We investigated the effect of Punica granatum peel aqueous extract (PGE), on pulmonary inflammation and alveolar degradation induced by intratracheal administration of Elastase in Sprague Dawley rats. Lung inflammation was induced in rats by intratracheal instillation of Elastase. On day 1 and 2, animals received an intraperitoneal injection of PGE (200 mg/mL), three hours later, they were intratracheally instilled with 25U/kg pancreatic porcine Elastase. Animals were sacrificed 7 days later. Bronchoalveolar lavage (BAL) were collected and cellularity, histology and mRNA expression of Monocyte chemotactic protein 1(MCP-1), Tumor Necrosis Factor-Alpha (TNF-α), Interleukin 6 (IL-6), and Matrix Metalloproteinase-2 (MMP-2) were studied. In addition, activity of TNF-α, IL-6 and MCP-1 on BAL were also analyzed by ELISA Kit. Elastase administration increased: BAL cellularity, neutrophils recruitment and BAL MCP-1, IL-6 expressions. It also increased lung TNF-α, MCP-1, MMP-2 expressions, platelets recruitment, histological parameters at 7 th day of elastase treatment. Intraperitoneal injection of 200 mg/kg of PGE reduced, significantly, BAL cellularity, and neutrophils recruitment. However, in animal treated with PGE, MCP-1, MMP-2 and IL-6 on day 7, were similar to the Sham group. Treatment with PGE (200 mg/ kg) also significantly reduced lung TNF-α, and MCP-1 expression. This study reveals that PGE Punica granatum protects against elastase lung inflammation and alveolar degradation induced in rats.

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Rectal pre-treatment with ozonized oxygen (O3) aggravates clinic status in septic rats treated with amoxicillin/clavulanate

Cited by 5 publications

References 31 publications

Effects of antibiotic and intra-peritoneal ozone administration on proinflammatory cytokine formation, antioxidant levels and abdominal organ functions in the treatment of experimentally generated infectious peritonitis in rabbits

Effects of antibiotic and intra-peritoneal ozone administration on proinflammatory cytokine formation, antioxidant levels and abdominal organ functions in the treatment of experimentally generated infectious peritonitis in rabbits

The effects of ozone on bacterial growth and thiol-disulphide homeostasis in vascular graft infection caused by MRSA in rats

An aqueous pomegranate peel extract (Punica granatum) protect against Elastase-induced pulmonary emphysema in Sprague Dawley rats model

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