Rectifying cow infertility under heat stress by immunization against inhibin and supplementation of progesterone

Chen, Fang; Liang, Jinghui; Guo, Rihong; Cheng, Ming; Guo, Binbin; Li, Wenqing; Tsigkou, Anastasia; Shi, Zhen‐Dan

doi:10.1016/j.domaniend.2022.106726

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…The cattle with multiple ovulatory follicles have an increased probability of multiple ovulations and a higher probability of twinning rate. Previous studies reported that immunization against INH enhanced embryo quality after superovulation and insemination in Holstein heifers ( 31 , 32 ). The oocyte maturation rate and parthenogenic embryo development potency were increased after treatment with anti-INH α subunit antibodies in vitro ( 33 ).…”

Section: Discussionmentioning

confidence: 97%

Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle

Meng,

Li,

Xiao

et al. 2024

Front. Endocrinol.

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The objective of the present study was to investigate the potential role of immunization against INH on follicular development, serum reproductive hormone (FSH, E2, and P4) concentrations, and reproductive performance in beef cattle. A total of 196 non-lactating female beef cattle (4-5 years old) with identical calving records (3 records) were immunized with 0.5, 1.0, 1.5, or 2.0 mg [(T1, n = 58), (T2, n = 46), (T3, n = 42) and (T4, n = 36), respectively] of the pcISI plasmid. The control (C) group (n = 14) was immunized with 1.0 mL 0.9% saline. At 21d after primary immunization, all beef cattle were boosted with half of the primary immunization dose. On day 10 after primary immunization, the beef cattle immunized with INH DNA vaccine evidently induced anti-INH antibody except for the T1 group. The T3 group had the greatest P/N value peak among all the groups. The anti-INH antibody positive rates in T2, T3 and T4 groups were significantly higher than that in C and T1 groups. RIA results indicated that serum FSH concentration in T2 group increased markedly on day 45 after booster immunization; the E2 amount in T3 group was significantly increased on day 10 after primary immunization, and the levels of E2 also improved in T2 and T3 groups after booster immunization; the P4 concentration in T2 group was significantly improved on day 21 after primary immunization. Ultrasonography results revealed that the follicles with different diameter sizes were increased, meanwhile, the diameter and growth speed of ovulatory follicle were significantly increased. Furthermore, the rates of estrous, ovulation, conception, and twinning rate were also significantly enhanced. These findings clearly illustrated that INH DNA vaccine was capable of promoting the follicle development, thereby improving the behavioral of estrous and ovulation, eventually leading to an augment in the conception rates and twinning rate of beef cattle.

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Section: Discussionmentioning

confidence: 97%

Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle

Meng,

Li,

Xiao

et al. 2024

Front. Endocrinol.

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“…The serum estradiol levels provide further evidence that serum levels are always a good index of follicular status. Minor changes in P 4 concentration can influence embryo development, as experimentally induced P 4 concentration reductions altered follicular steroidogenesis and enhanced uterine PGF2α production [30]. It has been claimed that extreme environmental conditions along with under nutrition may be the cause of buffalo's protracted anestrous phases, in addition to delayed ovulation causing changes in the preovulatory follicle microenvironment reduced levels of steroids may be due to a decrease in the availability of the cholesterol precursor [31].…”

Section: Discussionmentioning

confidence: 99%

Impact of Reproductive Status, Body Condition Score, and Locality on Hormonal, and Some Blood Metabolites in Egyptian Buffaloes

Soliman,

El-sanea,

Kandil

et al. 2024

Egyptian Journal of Veterinary Sciences

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HIS STUDY was designed to find out the influence of reproductive status, body condition, and localities on hormonal profile, antioxidant status, trace elements, blood biochemical, and metabolites in Egyptian buffaloes. Three experiments were conducted, in experiment 1; buffalo were divided into two groups according to localities (Fayoum & Behira). In experiment 2, 97 buffalo were divided into cyclic (n= 29), early pregnant (n=43), inactive ovaries (n= 12), and endometritis (n= 13). In experiment 3, according to body condition score, buffaloes were divided into three groups (Low, medium, and high). Blood samples were collected from all animals for analyzing progesterone (P 4 ), estrogen (E 2 ), superoxide dismutase (SOD), malondialdehyde (MDA), total protein (TP), albumin (Alb), total cholesterol, triglycerides, low-density lipoprotein (LDL), glucose, Zinc (Zn), and copper (Cu). Results indicated that P 4 , E 2 , antioxidant, TP, Alb, Glob, A/G ratio, glucose, Zn and Cu levels significantly increased (P<0.05) for buffaloes raised at Behira (Delta) compared to Fayoum (Upper Egypt). In pregnant and cyclic buffalo, P 4 , E 2 , antioxidant, TP, Glob, A/G ratio, cholesterol, triglycerides, glucose, SOD, Zn and Cu increased (P<0.05) in comparison to those with inactive ovaries or endometritis. Moreover, buffaloes with high and medium body conditions had higher (P < 0.05) levels of P 4 , E 2 , TP, Alb, glob, total cholesterol, triglycerides, and Cu than those having low body condition score. MDA and LDL levels were high (P<0.05) in animals with low body condition score. In conclusion, blood serum concentrations of P 4 , E 2 , total protein, albumin, globulin, total cholesterol, LDL, triglycerides, Zn, and Cu were the most sensitive biochemical markers to represent the reproductive status of the animals. The body condition score and locality have impacted the fertility of dairy buffalo.

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“…By increasing the circulating follicle-stimulating hormone (FSH) concentrations, the bolstering estrus behavior, and enhancing the oocyte and early embryo development competence in dairy cows [9], water buffaloes [10], and pigs [11], a method of immunoneutralization of inhibin bioactivity has been developed in recent decades to effectively stimulate or enhance the granulosa cells and ovarian follicle development. In addition to increasing the conception rate, immunization against inhibin, when combined with the OvSynch protocol, also increased the plasma concentrations of the interferon tau (IFN-tau) in dairy cows around the time of pregnancy recognition, further demonstrating its efficacy in enhancing early embryo development and oocyte maturation [12,13].…”

Section: Introductionmentioning

confidence: 99%

Activin A Reduces Porcine Granulosa Cells Apoptosis via ERβ-Dependent ROS Modulation

Chen

Zhu

2022

Veterinary Sciences

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Unfavorable conditions compromise animal reproduction by altering the ovarian granulosa cells’ follicular dynamics and normal physiological function (GCs), eventually resulting in oxidative damage and cell apoptosis. Activin is produced in the GCs and plays a vital role in folliculogenesis. This study investigated the effects of activin A (ACT-A) treatment in vitro on the apoptosis of porcine GCs and the underlying molecular mechanism. We found that ACT-A could attenuate the apoptosis of the GCs and enhance the synthesis of estrogen (E2). ACT-A also enhanced FSH-induced estrogen receptor-β (ERβ) expression, inhibiting ERβ aggravated intracellular accumulation of the reactive oxygen species (ROS) and apoptosis. The E2 levels in the culture medium, the mRNA expression pattern of the apoptosis-related genes (CASPASE 3, BCL2, and BAX), steroidogenesis-related gene (CYP19A1), and cell viability were analyzed to confirm the results. In summary, this study indicated the protective role of ACT-A in apoptosis by attenuating the ROS accumulation through ERβ. These results aim to enhance the follicular functions and improve animal reproductive performance.

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Rectifying cow infertility under heat stress by immunization against inhibin and supplementation of progesterone

Cited by 5 publications

References 52 publications

Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle

Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle

Impact of Reproductive Status, Body Condition Score, and Locality on Hormonal, and Some Blood Metabolites in Egyptian Buffaloes

Activin A Reduces Porcine Granulosa Cells Apoptosis via ERβ-Dependent ROS Modulation

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