Recurrent Haemoptysis? Think of Paragonimiasis

Razaque, M A; Mutum, Samarendra Singh; Singh, Takhelmayum Hemjit

doi:10.1177/004947559102100406

Cited by 8 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations appear to support the results of others [1,9]. The present study also shows that common symptoms are similar to those reported elsewhere [10-12]. …”

Section: Discussionsupporting

confidence: 94%

An epidemiological study of pleuropulmonary paragonimiasis among pupils in the peri-urban zone of Kumba town, Meme Division, Cameroon

et al. 2003

View full text Add to dashboard Cite

show abstract

“…These observations appear to support the results of others [1,9]. The present study also shows that common symptoms are similar to those reported elsewhere [10-12]. …”

Section: Discussionsupporting

confidence: 94%

An epidemiological study of pleuropulmonary paragonimiasis among pupils in the peri-urban zone of Kumba town, Meme Division, Cameroon

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Beside P. westermani, In the context of India, Chandler and Read (1961) indicated Bengal, Assam and some other parts of the country as endemic foci of human paragonimiasis. In recent years, this infection has been reported in a sizeable human population of Manipur, a northeastern state of the country (Razaque et al 1991;Singh et al 1993). Although the fluke is known to parasitize a wide range of mammalian hosts representing as many as 11 families, the status of its prevalence and host range in India is not well documented.…”

Section: Introductionmentioning

confidence: 96%

Surface fine topography and PCR-based determination of metacercaria of Paragonimus sp. from edible crabs in Arunachal Pradesh, Northeast India

et al. 2007

View full text Add to dashboard Cite

In several mountainous regions of Northeastern India, foci of Paragonimus infection reportedly involving species that are known to prevail in China have been identified. The present study was undertaken to demonstrate the surface fine topography and sequence analysis of the ribosomal deoxyribonucleic acid (rDNA; second internal transcribed spacer, ITS2) of the metacercarial stages of the lung fluke collected from a mountain stream of the area (Miao, Changlang District in Arunachal Pradesh). The encysted metacercariae were oval in shape and had a smooth surface. The newly excysted metacercaria had a ventral sucker larger than the oral; the body surface was covered with numerous single-pointed and thorn-like tegumentary spines, of which those on the anterior part of the body were bigger in size and showed a gradual reduction in length and number towards the posterior end; dome-shaped papillae in variable numbers were seen around the rim of the oral sucker and were sparsely distributed all over the body surface. The polymerase chain reaction-amplified rDNA ITS2 sequences of the metacercariae were aligned with known sequences for the various species of Paragonimus, and the expectation value was found to be most significant with P. westermani, revealing an absolute match. The surface topography including the number and distribution of papillae and spination patterns and the ITS2 sequences of the metacercariae strongly suggest that the Paragonimus species, prevalent in the region of India, is in fact P. westermani.

show abstract

“…This infection has been reported in a sizeable human population of Manipur, a north-eastern state of India [6,7]. Very recently in Manipur and Arunachal Pradesh (Northeast India), the suspected foci of human infection where consumption of crustacean intermediate hosts is of regular practice, the Chinese species, P. hueitungensis and P. heterotremus , respectively were identified as etiological agents of paragonimiasis [8,9].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic reconstruction using secondary structures and sequence motifs of ITS2 rDNA of Paragonimus westermani (Kerbert, 1878) Braun, 1899 (Digenea: Paragonimidae) and related species

et al. 2009

View full text Add to dashboard Cite

BackgroundMost phylogenetic studies using current methods have focused on primary DNA sequence information. However, RNA secondary structures are particularly useful in systematics because they include characteristics that give "morphological" information, not found in the primary sequence. In several mountainous regions of Northeastern India, foci of Paragonimus (lung fluke) infection reportedly involve species that are known to prevail in neighbouring countries. The present study was undertaken to demonstrate the sequence analysis of the ribosomal DNA (ITS2) of the infective (metacercarial) stage of the lung fluke collected from the edible crab hosts that are abundant in a mountain stream of the area (Miao, Changlang District in Arunachal Pradesh) and to construct its phylogeny. Using the approach of molecular morphometrics that is based on ITS2 secondary structure homologies, phylogenetic relationships of the various isolates of Paragonimus species that are prevalent in the neighbouring Near-eastern countries have been discussed.ResultsInitially, ten predicted RNA secondary structures were reconstructed and the topology based only on the predicted RNA secondary structure of the ITS2 region resolved most relationships among the species studied. We obtained three similar topologies for seven species of the genus Paragonimus on the basis of traditional primary sequence analysis using MEGA and a Bayesian analysis of the combined data. The latter approach allowed us to include both primary sequence and RNA molecular morphometrics; each data partition was allowed to have a different evolution rate. Paragonimus westermani was found to group with P. siamensis of Thailand; this was best supported by both the molecular morphometrics and combined analyses. P. heterotremus, P. proliferus, P. skrjabini, P. bangkokensis and P. harinasutai formed a separate clade in the molecular phylogenies, and were reciprocally monophyletic with respect to other species. ITS2 sequence motifs allowed an accurate in-silico distinction of lung flukes.ConclusionData indicate that ITS2 motifs (≤ 50 bp in size) can be considered a promising tool for trematode species identification. RNA secondary structure analysis could be a valuable tool for distinguishing new species and completing Paragonimus systematics, more so because ITS2 secondary structure contains more information than the usual primary sequence alignment.

show abstract

Recurrent Haemoptysis? Think of Paragonimiasis

Cited by 8 publications

References 3 publications

An epidemiological study of pleuropulmonary paragonimiasis among pupils in the peri-urban zone of Kumba town, Meme Division, Cameroon

An epidemiological study of pleuropulmonary paragonimiasis among pupils in the peri-urban zone of Kumba town, Meme Division, Cameroon

Surface fine topography and PCR-based determination of metacercaria of Paragonimus sp. from edible crabs in Arunachal Pradesh, Northeast India

Phylogenetic reconstruction using secondary structures and sequence motifs of ITS2 rDNA of Paragonimus westermani (Kerbert, 1878) Braun, 1899 (Digenea: Paragonimidae) and related species

Contact Info

Product

Resources

About