Recurrent outbreaks of Serratia marcescens among neonates and infants at a pediatric department: an outbreak analysis

Ivády, Balázs; Szabó, Dóra; Damjanova, Ivelina; Pataki, M; Szabó, Marcell; Kenesei, Éva

doi:10.1007/s15010-014-0654-9

Cited by 20 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S erratia marcescens es un importante patógeno causante de infecciones asociadas a la atención de salud (IAAS) en las unidades de cuidados intensivos neonatales (UCIN) con significativas tasas de mortalidad 1,2 . El bajo peso al nacer, la estancia prolongada, el uso de dispositivos médi-cos invasores y la utilización previa de antimicrobianos son considerados factores de riesgo para su adquisición 3 .…”

Section: Introductionunclassified

Brote por Serratia marcescens en una Unidad de Cuidados Intensivos Neonatales: Guayaquil-Ecuador

Soria¹,

Nieto²,

Villacís

et al. 2016

Rev. chil. infectol.

View full text Add to dashboard Cite

Serratia marcescens outbreak in Neonatal IntensiveCare Unit. Guayaquil, EcuadorWe report a Serratia marcescens outbreak occurred in the NICU of a pediatric hospital in Guayaquil, Ecuador. Nine cases of infection were detected, from which septicemia was developed in 55.5%. The index case was a newborn derived from another institution with septic arthritis caused by the outbreak strain. The infection rate was 17.6% and mortality rate was 33.3%. All isolates were resistant to aminoglycosides and susceptible to third generation cephalosporins and carbapenems. Clonality analysis by pulsed-field gel electrophoresis (PFGE) revealed the presence of two closely related clones confirming the horizontal spread. Measures were taken by the committee such as: strengthening the hand hygiene, patient hygiene and cohort studies of gastrointestinal colonization, which allowed the control of the outbreak.

show abstract

Section: Introductionunclassified

Brote por Serratia marcescens en una Unidad de Cuidados Intensivos Neonatales: Guayaquil-Ecuador

Soria¹,

Nieto²,

Villacís

et al. 2016

Rev. chil. infectol.

View full text Add to dashboard Cite

show abstract

“…Bacterium-specific PCR has not been widely used in the diagnosis of meningitis in newborn babies since it can be caused by many types of bacteria: some more common (e.g. Group B Streptococcus, Escherichia coli) [12][13][14]35], some less so [23][24][25][26][27][28][29][30][31][32][39][40][41][42][43][44][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

et al. 2020

View full text Add to dashboard Cite

Background: PCRctic is an innovative assay based on 16S rDNA PCR technology that has been designed to detect a single intact bacterium in a specimen of cerebro-spinal fluid (CSF). The assay's potential for accurate, fast and inexpensive discrimination of bacteria-free CSF makes it an ideal adjunct for confident exclusion of bacterial meningitis in newborn babies where the negative predictive value of bacterial culture is poor. This study aimed to stress-test and optimize PCRctic in the "field conditions" to attain a clinically useful level of specificity. Methods: The specificity of PCRctic was evaluated in CSF obtained from newborn babies investigated for meningitis on a tertiary neonatal unit. Following an interim analysis, the method of skin antisepsis was changed to increase bactericidal effect, and snap-top tubes (Eppendorf™) replaced standard universal containers for collection of CSF to reduce environmental contamination. Results: The assay's specificity was 90.5% in CSF collected into the snap-top tubesup from 60% in CSF in the universal containers. The method of skin antisepsis had no effect on the specificity. All CSF cultures were negative and no clinical cases of neonatal bacterial meningitis occurred during the study. Conclusions: A simple and inexpensive optimization of CSF collection resulted in a high specificity output. The low prevalence of neonatal bacterial meningitis means that a large multi-centre study will be required to validate the assay's sensitivity and its negative predictive value.

show abstract

“…Bacterium-speci c PCR has not been widely used in the diagnosis of meningitis in newborn babies since it can be caused by many types of bacteria: some more common (e.g. Group B Streptococcus, Escherichia coli) [12][13][14]25], some less so [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay.

Abelian

Mund

Curran

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: PCRctic is an innovative assay based on 16S rDNA PCR technology that has been designed to detect a single intact bacterium in a specimen of cerebro-spinal fluid (CSF). The assay’s potential for accurate, fast and inexpensive discrimination of bacteria-free CSF makes it an ideal adjunct for confident exclusion of bacterial meningitis in newborn babies where the negative predictive value of bacterial culture is poor. This study aimed to stress-test and optimize PCRctic in the “field conditions” to attain a clinically useful level of specificity.Methods: The specificity of PCRctic was evaluated in CSF obtained from newborn babies investigated for meningitis on a tertiary neonatal unit. Following an interim analysis, the method of skin antisepsis was changed to increase bactericidal effect, and snap-top tubes (EppendorfTM) replaced standard universal containers for collection of CSF to reduce environmental contamination.Results: The assay’s specificity was 90.5% in CSF collected into the snap-top tubes – up from 60% in CSF in the universal containers. The method of skin antisepsis had no effect on the specificity. All CSF cultures were negative and no clinical cases of neonatal bacterial meningitis occurred during the study. Conclusions: a simple and inexpensive optimization of CSF collection resulted in a high specificity output. The low prevalence of neonatal bacterial meningitis means that a large multi-centre study will be required to validate the assay’s sensitivity and its negative predictive value.

show abstract

Recurrent outbreaks of Serratia marcescens among neonates and infants at a pediatric department: an outbreak analysis

Cited by 20 publications

References 31 publications

Brote por Serratia marcescens en una Unidad de Cuidados Intensivos Neonatales: Guayaquil-Ecuador

Brote por Serratia marcescens en una Unidad de Cuidados Intensivos Neonatales: Guayaquil-Ecuador

Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay.

Contact Info

Product

Resources

About