Recurrent Streptococcus equi subsp. zooepidemicus Bacteremia in an Infant

Watson, Joshua R.; Leber, Amy; Velineni, Sridhar; Timoney, John F.; Ardura, Monica I.

doi:10.1128/jcm.01306-15

Cited by 6 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few studies have analyzed SzM of S. equi subsp. zooepidemicus isolated from diverse clinical manifestations or host species (8,16,18,36). Two SzM sequences of S. equi subsp.…”

Section: Discussionmentioning

confidence: 99%

Prominent Binding of Human and Equine Fibrinogen to Streptococcus equi subsp. zooepidemicus Is Mediated by Specific SzM Types and Is a Distinct Phenotype of Zoonotic Isolates

et al. 2019

View full text Add to dashboard Cite

Streptococcus equi subsp. zooepidemicus is an important pathogen in horses that causes severe diseases such as pneumonia and abortion. Furthermore, it is a zoonotic agent, and contact with horses is a known risk factor. In this study, we investigated the working hypothesis that the zoonotic potential varies among S. equi subsp. zooepidemicus strains in association with differences in M-like protein-mediated binding of host plasma proteins. We demonstrate via in-frame deletion mutagenesis of two different S. equi subsp. zooepidemicus strains that the M-like protein SzM is crucial for the binding of fibrinogen to the bacterial surface and for survival in equine and human blood. S. equi subsp. zooepidemicus isolates of equine and human origins were compared with regard to SzM sequences and binding of equine and human fibrinogens. The N-terminal 216 amino acids of the mature SzM were found to exhibit a high degree of diversity, but the majority of human isolates grouped in three distinct SzM clusters. Plasma protein absorption assays and flow cytometry analysis revealed that pronounced binding of human fibrinogen is a common phenotype of human S. equi subsp. zooepidemicus isolates but much less so in equine S. equi subsp. zooepidemicus isolates. Furthermore, binding of human fibrinogen is associated with specific SzM types. These results suggest that SzM-mediated binding of human fibrinogen is an important virulence mechanism of zoonotic S. equi subsp. zooepidemicus isolates.

show abstract

“…Few studies have analyzed SzM of S. equi subsp. zooepidemicus isolated from diverse clinical manifestations or host species (8,16,18,36). Two SzM sequences of S. equi subsp.…”

Section: Discussionmentioning

confidence: 99%

Prominent Binding of Human and Equine Fibrinogen to Streptococcus equi subsp. zooepidemicus Is Mediated by Specific SzM Types and Is a Distinct Phenotype of Zoonotic Isolates

et al. 2019

View full text Add to dashboard Cite

show abstract

“…MLST and PFGE have been used in epidemiologic investigations of SEZ to determine the source of human infections, i.e. if the zoonosis resulted from direct contact with animals or from the consumption of dairy products [4,5,12,16,37,[43][44][45]. PubMLST global database of Streptococcus zooepidemicus currently includes 830 SEZ isolates (http://pubmlst.org/ szooepidemicus), most from Europe (77%).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to β-lactams, human isolates are commonly susceptible to other families of antibiotics such as glycopeptides, fluoroquinolones, daptomycin and linezolid. However, susceptibility to tetracyclines, macrolides, clindamycin, rifamycins and sulfonamides is highly variable among different isolates [2,[13][14][15][16]. In the case of tetracycline, one of the strategies responsible for this antibiotic resistance is the specific ribosomal protection, whose most common enzymes are encoded by tetM and tetO genes [17].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Streptococcus equi subsp. zooepidemicus isolates containing lnuB gene responsible for the L phenotype

Azpiroz¹,

Burger²,

Mazza³

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Within the framework of the β-hemolytic streptococci surveillance carried out by the National Reference Laboratory from Uruguay, three putative Streptococcus equi subsp. zooepidemicus (SEZ) were received from different health centers. Being these the first reports associated with human infections in Uruguay, the objective of this work was to confirm their identification, to determine their genetic relationship and to study their antibiotic susceptibility. Using four different methods, they were identified as SEZ, a subspecies which has been described as the etiologic agent of rare and severe zoonosis in a few cases in other countries. The three isolates presented different pulsotypes by PFGE; however, two of them appeared to be related and were confirmed as ST431 by MLST, while the remaining isolate displayed ST72. Their resistance profile exhibited an unexpected feature: despite all of them were susceptible to macrolides, they showed different levels of resistance to clindamycin, i.e. they had the so-called “L phenotype”. This rare trait is known to be due to a nucleotidyl-transferase, encoded by genes of the lnu family. Although this phenotype was previously described in a few SEZ isolates, its genetic basis has not been studied yet. This was now analyzed by PCR in the three isolates and they were found to contain a lnuB gene. The lnuB sequence was identical among the three isolates and with many lnuB sequences deposited in data banks. In conclusion, for the first time in Uruguay, three SEZ isolates recovered from non-epidemiologically related cases of human invasive infection were identified. Moreover, this is the first report about the presence of a lnu gene in the S. equi species, revealing the active lateral spread of the lnuB in a new streptococcal host.

show abstract

“…Strains were also susceptible to ceftriaxone or levofloxacin in combination with rifampicin [92,95]. [196] Streptococcus iniae S. iniae are most often isolated from aquatic animals, mainly fish and marine mammals [96,97]. This species is characterized by β-haemolysis, which can be diffused by the outer ring of α-haemolysis [98].…”

Section: Streptococcus Equi Subsp Zooepidemicus (Sez)mentioning

confidence: 99%

Pathogenicity and drug resistance of animal streptococci responsible for human infections

Glajzner

Szewczyk

Szemraj

2021

Journal of Medical Microbiology

View full text Add to dashboard Cite

Bacteria of the genus Streptococcus , earlier considered typically animal, currently have also been causing infections in humans. It is necessary to make clinicians aware of the emergence of new species that may cause the development of human diseases. There is an increasing frequency of isolation of streptococci such as S. suis , S. dysgalactiae , S. iniae and S. equi from people. Isolation of Streptococcus bovis/Streptococcus equinus complex bacteria has also been reported. The streptococcal species described in this review are gaining new properties and virulence factors by which they can thrive in new environments. It shows the potential of these bacteria to changes in the genome and the settlement of new hosts. Information is presented on clinical cases that concern streptococcus species belonging to the groups Bovis, Pyogenic and Suis. We also present the antibiotic resistance profiles of these bacteria. The emerging resistance to β-lactams has been reported. In this review, the classification, clinical characteristics and antibiotic resistance of groups and species of streptococci considered as animal pathogens are summarized.

show abstract

Recurrent Streptococcus equi subsp. zooepidemicus Bacteremia in an Infant

Cited by 6 publications

References 19 publications

Prominent Binding of Human and Equine Fibrinogen to Streptococcus equi subsp. zooepidemicus Is Mediated by Specific SzM Types and Is a Distinct Phenotype of Zoonotic Isolates

Prominent Binding of Human and Equine Fibrinogen to Streptococcus equi subsp. zooepidemicus Is Mediated by Specific SzM Types and Is a Distinct Phenotype of Zoonotic Isolates

Characterization of Streptococcus equi subsp. zooepidemicus isolates containing lnuB gene responsible for the L phenotype

Pathogenicity and drug resistance of animal streptococci responsible for human infections

Contact Info

Product

Resources

About