Recurrent vulvovaginal Candida spp isolates phenotypically express less virulence traits

Faria-Gonçalves, Paula; Rolo, Joana; Gaspar, Carlos; Oliveira, Ana Sofia; Pestana, Paula Gouveia; Palmeira‐de‐Oliveira, Rita; Gonçalves, Teresa; Martinez-de-Oliveira, José; Palmeira‐de‐Oliveira, Ana

doi:10.1016/j.micpath.2020.104471

Cited by 13 publications

(13 citation statements)

References 21 publications

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“…Interestingly, by comparatively evaluating isolates obtained from two clinical groups of VVC and cVVC, Faria-Gonc ¸alves et al found that virulence factors (biofilm formation, germ tube formation and phospholipase production) were more expressed in the VVC group in relation to the cVVR group. The hypothesis was raised that these strains by reducing the expression of virulence factors may more easily escape the immune system and may persist [73].…”

Section: Consolaro Et Al Point To a Possible Relationship Between The Production Of Proteolytic Enzymes And Adhesion Of Cells To The Mucomentioning

confidence: 99%

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Virulence Factors as Promoters of Chronic Vulvovaginal Candidosis: A Review

et al. 2021

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Section: Consolaro Et Al Point To a Possible Relationship Between The Production Of Proteolytic Enzymes And Adhesion Of Cells To The Mucomentioning

confidence: 99%

“…Hence, phospholipase production seems to be an important virulence factor for Candida spp. mucosal infection in general [73].…”

Section: Production Of Extracellular Enzymesmentioning

confidence: 99%

Virulence Factors as Promoters of Chronic Vulvovaginal Candidosis: A Review

et al. 2021

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“…On the other hand, in the sVVC group, a significant correlation ( p < 0.05) was observed between the proportion of viable internalized yeasts and the level of hydrophobicity of yeast cell walls. In the epithelial-adherence test (co-culture with HeLa cells), we found a significant correlation ( p < 0.05) between the number of CFU/mL recovered after incubation and the ability to form biofilms in-vitro (as determined in Table 1 [ 10 ]) only for the sVVC clinical group ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…The study was approved by the Ethics Committee of the research centre where the experiments were conducted, CICS-UBI (approval CE-UBI-Pj-2018-022). The strains were described in previous studies, in regard to their susceptibility to fluconazole and ability to form biofilms [ 3 , 10 ] ( Table 1 ). The identification of the Candida albicans isolates was obtained using gold-standard techniques routinely used for identification in clinical laboratories.…”

Section: Methodsmentioning

confidence: 99%

“…to persist in the vaginal mucosa are largely elusive. Our previous studies have identified that chronic Candida isolates are weak biofilm and germ tube producers, when compared with isolates involved in sporadic infections [ 10 ]. A recent study has revealed that vaginal isolates show adaptative behavior in their interaction with macrophages in-vitro, reflecting their infective ability [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Vulvovaginal Candida albicans Clinical Isolates’ Resistance to Phagocytosis In-Vitro

Faria-Gonçalves

Oliveira

Gaspar

et al. 2022

Life

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Previous studies have revealed that Candida albicans isolates involved in chronic vulvovaginal candidosis (cVVC) phenotypically express less virulent traits than clinical isolates involved in sporadic infections. In this study, we aimed to further explore this finding by studying the behaviour of those same clinical isolates in in-vitro models of infection. Eighteen clinical Candida albicans isolates were collected from women suffering sporadic (eight isolates) or chronic infections (ten isolates). Adhesion to HeLa cells (human cervical cancer epithelial cell line) and resistance to phagocytosis by RAW 264.7 cells (murine macrophages cell line) were tested in-vitro. In addition, phenotypic expression of virulence factors related with either adhesion or resistance to phagocytosis was tested in-vitro. Results indicated that yeast isolates involved in sporadic infection adhered in a higher proportion of HeLa cells than those of chronic infections, which was related with their ability to produce biofilm (p < 0.05). The ability to evade phagocytosis was related to an elevated production of proteases (p < 0.05) by chronic isolates, while sporadic isolates’ resistance to phagocytosis was related to a higher hydrophobicity of cell walls (p < 0.05). We conclude that the evasion of macrophage-mediated phagocytosis related to the production of proteases might be an important factor involved in the recurrence of vulvovaginal candidosis infection.

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Humoral immune responses against facultative pathogen Candida utilis in atopic patients with vulvovaginal candidiasis. Candida utilis glucomannan – New serologic biomarker

Paulovičová

Hrubiško

2022

Immunobiology

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Recurrent vulvovaginal Candida spp isolates phenotypically express less virulence traits

Cited by 13 publications

References 21 publications

Virulence Factors as Promoters of Chronic Vulvovaginal Candidosis: A Review

Virulence Factors as Promoters of Chronic Vulvovaginal Candidosis: A Review

Vulvovaginal Candida albicans Clinical Isolates’ Resistance to Phagocytosis In-Vitro

Humoral immune responses against facultative pathogen Candida utilis in atopic patients with vulvovaginal candidiasis. Candida utilis glucomannan – New serologic biomarker

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