Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang, Xianrui; Li, Li; Xiao, Li; Zhang, Donghui

doi:10.1186/s13287-018-1094-8

Cited by 51 publications

(35 citation statements)

References 88 publications

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“…The basic tissue engineering procedures consist of appropriate scaffold manufacture, hydrogel matrix support, and patterning design. The combination of these approaches promotes the development of the desired complex, both in tissue structure and function[ 20 , 80 ].…”

Section: Different Types Of Scaffold Formulationsmentioning

confidence: 99%

“…The therapeutic role of DSCs in combination with various scaffolds has been extensively investigated in restoring tooth damage or loss due to caries, periodontal disease, trauma, or genetic disorders[ 89 ]. The procedure of dentin formation consists of odontoblastic deposition, vascularization, and neuron formation[ 20 ]. Among multiple approaches to promote dentin formation and teeth tissue regeneration, the application of DSCs with a synthetic pre-designed and optimized scaffold is the most accepted technique for tooth regeneration[ 90 ].…”

Section: Tooth Reconstructionmentioning

confidence: 99%

“…One of the unique characteristics of DSCs is their ability to differentiate into mesodermal, ectodermal, and endodermal cell lineages[ 18 ]. DSCs from each source are capable of specifically differentiating into various distinct cells, including epithelial cells, odontoblasts, osteoblasts, chondroblasts, adipocytes, vascular cells, endotheliocytes, neuronal cells, glial cells, photoreceptor cells, and muscle cells[ 19 , 20 ]. Although all stem cells obtained from various sources are named DSCs in this study, their phenotype, differentiation potential (both in in vitro and in vivo conditions) and functional properties (such as biological response during differentiation and tissue repair) are different[ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

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Dental stem cells (DSCs) are self-renewable cells that can be obtained easily from dental tissues, and are a desirable source of autologous stem cells. The use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation, high plasticity, immunomodulatory properties, and multipotential abilities. Using appropriate scaffolds loaded with favorable biomolecules, such as growth factors, and cytokines, can improve the proliferation, differentiation, migration, and functional capacity of DSCs and can optimize the cellular morphology to build tissue constructs for specific purposes. An enormous variety of scaffolds have been used for tissue engineering with DSCs. Of these, the scaffolds that particularly mimic tissue-specific micromilieu and loaded with biomolecules favorably regulate angiogenesis, cell-matrix interactions, degradation of extracellular matrix, organized matrix formation, and the mineralization abilities of DSCs in both in vitro and in vivo conditions. DSCs represent a promising cell source for tissue engineering, especially for tooth, bone, and neural tissue restoration. The purpose of the present review is to summarize the current developments in the major scaffolding approaches as crucial guidelines for tissue engineering using DSCs and compare their effects in tissue and organ regeneration.

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Section: Different Types Of Scaffold Formulationsmentioning

confidence: 99%

Section: Tooth Reconstructionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

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“…Human dental pulp stem cells (hDPSCs) are a candidate for this purpose. hDPSCs can be easily obtained from primary teeth lost during childhood or wisdom teeth extracted in adulthood [ 1 ]. hDPSCs are multipotent [ 2 , 3 ] and their facile collection supports regenerative medicine [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid differentiation of human dental pulp stem cells to neuron-like cells by high K<sup>+</sup> stimulation

et al. 2020

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As human-origin cells, human dental pulp stem cells (hDPSCs) are thought to be potentially useful for biological and medical experiments. They are easily obtained from lost primary teeth or extracted wisdom teeth, and they are mesenchymal stem cells that are known to differentiate into osteoblasts, chondrocytes, and adipocytes. Although hDPSCs originate from neural crest cells, it is difficult to induce hDPSCs to differentiate into neuron-like cells. To facilitate their differentiation into neuron-like cells, we evaluated various differentiation conditions. Activation of K + channels is thought to regulate the intracellular Ca 2+ concentration, allowing for manipulation of the cell cycle to induce the differentiation of hDPSCs. Therefore, in addition to a conventional neural cell differentiation protocol, we activated K + channels in hDPSCs. Immunocytochemistry and real-time PCR revealed that applying a combination of 3 stimuli (high K + solution, epigenetic reprogramming solution, and neural differentiation solution) to hDPSCs increased their expression of neuronal markers, such as β3-tubulin, postsynaptic density protein 95, and nestin within 5 days, which led to their rapid differentiation into neuron-like cells. Our findings indicate that epigenetic reprogramming along with cell cycle regulation by stimulation with high K + accelerated the differentiation of hDPSCs into neuron-like cells. Therefore, hDPSCs can be used in various ways as neuron-like cells by manipulating their cell cycle.

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“…It has been shown that different subsets of dental MSCs may be distinguished on the basis of the expression of surface markers 21 and show variable potential to regenerate dental/oral tissues in vitro and in vivo 17 . In contrast to other SC sources representing the gold standard in regenerative medicine, such as the bone marrow-and the adipose tissue-derived MSCs, dental MSCs have been shown to possess functional peculiarities that render them as the cell source of choice for dental TE 22 .…”

Section: Significancementioning

confidence: 99%

Prospects of Advanced Therapy Medicinal Products–Based Therapies in Regenerative Dentistry: Current Status, Comparison with Global Trends in Medicine, and Future Perspectives

Bakopoulou

2020

Journal of Endodontics

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Introduction: Regenerative medicine offers innovative approaches to restore damaged tissues on the basis of tissue engineering (TE). Although research on advanced therapy medicinal products (ATMPs) has been very active in recent years, the number of licensed products remains surprisingly low and restricted to the treatment of severe, incurable diseases. Methods: This paper provides a critical review of current literature on the regulatory, clinical, and commercial status of ATMP-based therapies in the EU and worldwide and the hurdles to overcome for their broader application in Regenerative Dentistry. Results: Competent authorities have focused on developing regulatory pathways to address unmet patient needs. Oncology represents the dominating field, followed by cardiovascular, musculoskeletal, neurodegenerative, immunologic, and inherited diseases. Yet, the status remains in early development, and scientific, regulatory, and cost-effectiveness issues impose considerable hurdles toward marketing authorization, technology adoption, and patient accessibility. In this context, although regenerative dentistry has achieved breakthrough innovations in TE of several dental/oral tissues in preclinical models, it has hardly harnessed research progress to integrate innovative regenerative treatments into clinical practice. Conclusion: Global demographic changes, which demonstrate a steady increase of the aging population, highlight the societal need for the application of ATMP-based therapies in the treatment of noncommunicable diseases (NCDs). Although oral diseases, as an integral part of NCDs, are not life-threatening and largely preventable, they sustain high prevalence, with severe burden on economy and quality of life. In this perspective, the urgent request to ultimately translate draining research in dental TE conducted during the last decades into innovative treatments brought safely and cost-effectively into society at large still holds the stage. This review provides an overview of the regulatory, clinical, and commercial status of ATMP-based therapies in the European Union and worldwide and the hurdles to overcome for their broader application in regenerative dentistry.

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Recycle the dental fairy’s package: overview of dental pulp stem cells

Cited by 51 publications

References 88 publications

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Rapid differentiation of human dental pulp stem cells to neuron-like cells by high K<sup>+</sup> stimulation

Prospects of Advanced Therapy Medicinal Products–Based Therapies in Regenerative Dentistry: Current Status, Comparison with Global Trends in Medicine, and Future Perspectives

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