2016
DOI: 10.1093/bioinformatics/btw651
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Recycler: an algorithm for detecting plasmids from de novo assembly graphs

Abstract: MotivationPlasmids and other mobile elements are central contributors to microbial evolution and genome innovation. Recently, they have been found to have important roles in antibiotic resistance and in affecting production of metabolites used in industrial and agricultural applications. However, their characterization through deep sequencing remains challenging, in spite of rapid drops in cost and throughput increases for sequencing. Here, we attempt to ameliorate this situation by introducing a new circular … Show more

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Cited by 126 publications
(90 citation statements)
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“…Detection and reconstruction of plasmids in the different L. mudanjiangensis strains 443 was performed using Recycler v0.7 [46], with the original fastq files and SPAdes 444 assembly graphs as input. The assembled plasmids were annotated with Prokka and 445 further characterized by scanning against the EggNOG database, as described in above.…”
Section: Plasmid Identification 442mentioning
confidence: 99%
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“…Detection and reconstruction of plasmids in the different L. mudanjiangensis strains 443 was performed using Recycler v0.7 [46], with the original fastq files and SPAdes 444 assembly graphs as input. The assembled plasmids were annotated with Prokka and 445 further characterized by scanning against the EggNOG database, as described in above.…”
Section: Plasmid Identification 442mentioning
confidence: 99%
“…the L. plantarum group, using a comparative genomics approach. Therefore, the 45 genome of the type strain of L. mudanjiangensis was sequenced together with three 46 strains isolated from fermented carrot juice. These and other publicly available genome 47 sequences were used to screen for L. mudanjiangensis species-specific properties, which 48 included an analysis for the presence of genes related to pili formation and conjugation.…”
mentioning
confidence: 99%
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“…Repeated sequences, often shared between plasmid and chromosomal DNA, hinder the assembly of the bacterial genome from short-read sequence data, often resulting in contigs of which the origin, either plasmid or chromosomal, cannot be resolved. In recent years, several tools to study plasmids using WGS data were developed and include PLACNET, PlasmidFinder, cBar, HyAsP, MOB-suite, PlasmidTron, Recycler, and PlasmidSPAdes ( [4][5][6][7][8][9][10][11]. Only Recycler and PlasmidSPAdes are fully automated computer programs that aim to de novo reconstruct whole plasmid sequences from short-read sequence data ( 7,8 ).…”
Section: Introductionmentioning
confidence: 99%
“…In recent years, several tools to study plasmids using WGS data were developed and include PLACNET, PlasmidFinder, cBar, HyAsP, MOB-suite, PlasmidTron, Recycler, and PlasmidSPAdes ( [4][5][6][7][8][9][10][11]. Only Recycler and PlasmidSPAdes are fully automated computer programs that aim to de novo reconstruct whole plasmid sequences from short-read sequence data ( 7,8 ). Recent studies benchmarked the PlasmidSPAdes and Recycler algorithm for genomes of Gram-negative bacteria ( 12,13 ).…”
Section: Introductionmentioning
confidence: 99%