Recycling of intact dense core vesicles in neurites of NGF‐treated PC12 cells

Bauer, Roslyn A.; Khera, Rebecca S; Lieber, Janet L.; Angleson, Joseph K.

doi:10.1016/j.febslet.2004.05.086

Cited by 15 publications

(13 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We concluded that the internalized membrane is recovered to releasable vesicles in Ͻ2 min. This conclusion is in agreement with the recent findings by Bauer et al (8) in NGF-treated PC12 cells, by Henkel et al (21) in bovine chromaffin cells, and by Fulop et al (17) in mouse chromaffin cells. These authors have shown that internalized granules that resemble large dense core vesicles show up in ϳ2 min after exocytosis and become releasable in Ͻ5 min.…”

Section: Discussionsupporting

confidence: 83%

“…It is commonly believed that the entire contents of dense core granules are released on exocytosis. However, existing evidence supports the notion that vesicles may actually release only a portion of these contents (1,3,8,9,32,37) before they are internalized (40). In pituitary lactotrophs, dense core vesicles undergoing exocytic fusion with the plasma membrane retained some nonsecreted prolactin in a dense core that was slowly internalized and then used during subsequent rounds of exocytosis (9).…”

Section: Discussionmentioning

confidence: 94%

“…In pituitary lactotrophs, dense core vesicles undergoing exocytic fusion with the plasma membrane retained some nonsecreted prolactin in a dense core that was slowly internalized and then used during subsequent rounds of exocytosis (9). PC12 cells retain and retrieve their vesicle cores and make them available for the subsequent exocytotic release in Ͻ5 min (8). Particularly in chromaffin cells, one-third of the granules fused in response to a 0.5-s depolarization resealed within 100 s (32), and the recaptured granules can retain vesicle proteins such as chromogranins and the tissue plasminogen activator (17,32).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid recovery of releasable vesicles and formation of nonreleasable endosomes follow intense exocytosis in chromaffin cells

Bay

Ibáñez²,

Marengo³

2007

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Perez Bay AE, Ibañ ez LI, Marengo FD. Rapid recovery of releasable vesicles and formation of nonreleasable endosomes follow intense exocytosis in chromaffin cells. Am J Physiol Cell Physiol 293: C1509-C1522, 2007. First published August 8, 2007 doi:10.1152/ajpcell.00632.2006.-Neurons and neuroendocrine cells must retrieve plasma membrane excess and refill vesicle pools depleted by exocytosis. To perform these tasks cells can use different endocytosis/recycling mechanisms whose selection will impact on vesicle recycling time and secretion performance. We used FM1-43 to evaluate in the same experiment exocytosis, endocytosis, and recovery of releasable vesicles on mouse chromaffin cells. Various exocytosis levels were induced by a variety of stimuli, and we discriminated the resultant endocytosis-recycling responses according to their ability to rapidly generate releasable vesicles. Exocytosis of Յ20% of plasma membrane (provoked by nicotine/acetylcholine) was followed by total recovery of releasable vesicles. If a stronger stimulus (50 mM K ϩ and 2 mM Ca 2ϩ ) provoking intense exocytosis (51 Ϯ 7%) was applied, endocytosis still retrieved all the fused membrane, but only a fraction (19 Ϯ 2%) was releasable by a second stimulus. Using ADVASEP-7 or bromophenol blue to quickly eliminate fluorescence from noninternalized FM1-43, we determined that this fraction became releasable in Ͻ2 min. The remaining nonreleasable fraction was distributed mainly as fluorescent spots (ϳ0.7 m) selectively labeled by 40-to 70-kDa dextrans and was suppressed by a phosphatidylinositol-3-phosphate kinase inhibitor, suggesting that it had been formed by a bulk retrieval mechanism. We concluded that chromaffin cells can rapidly recycle significant fractions of their total vesicle population, and that this pathway prevails when cholinergic agonists are used as secretagogues. When exocytosis exceeded ϳ20% of plasma membrane, an additional mechanism was activated, which was unable to produce secretory vesicles in our experimental time frame but appeared crucial to maintaining membrane surface homeostasis under extreme conditions. endocytosis; mouse chromaffin cells; calcium signal; FM1-43; ADVASEP-7; bromophenol blue

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid recovery of releasable vesicles and formation of nonreleasable endosomes follow intense exocytosis in chromaffin cells

Bay

Ibáñez²,

Marengo³

2007

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

“…However, existing evidence supports the notion that vesicles might actually release only a portion of these contents (Alés et al, 1999;Artalejo et al, 1998;Bauer et al, 2004a;Taraska et al, 2003) before they are internalized. PC12 cells retain and retrieve their vesicle cores and make them available for subsequent exocytic release within <5 min (Bauer et al, 2004b). In chromaffin cells, one-third of the granules fuse in response to depolarization and reseal within 100 s (Perrais et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Transient fusion ensures granule replenishment to enable repeated release after IgE-mediated mast cell degranulation

Balseiro-Gómez

Flores

Acosta

et al. 2016

Journal of Cell Science

View full text Add to dashboard Cite

To ensure normal immune function, mast cells employ different pathways to release mediators. Here, we report a thus far unknown capacity of mast cells to recycle and reuse secretory granules after an antigen-evoked degranulation process under physiological conditions; this phenomenon involves the existence of a recycling secretory granule pool that is available for release in a short time scale. Rapid endocytic modes contributed to the recycling of ∼60% of the total secretory granule population, which involved kiss-and-run and cavicapture mechanisms, causing retention of the intragranular matrix. We found the presence of normal-size granules and giant actomyosin-and dynamin-dependent granules, which were characterized by large quantal content. These large structures allowed the recovered mast cells to release a large amount of 5-HT, compensating for the decrease in the number of exocytosed secretory granules. This work uncovers a new physiological role of the exo-endocytosis cycle in the immunological plasticity of mast cells and reveals a new property of their biological secretion.

show abstract

“…Moreover, kiss-and-run exocytosis could be a means to regulate quantal size, with the open time of the fusion pore determining the amount of neurotransmitter released per vesicle. Finally, during kiss-and-run exocytosis, LDCV retain their dense-core, which is made up of granins [255], thereby preventing graninmediated feedback. Consequently, factors that regulate the dynamics of the fusion pore or control the choice between full fusion and kiss-and-run exocytosis are critical for neurotransmission and are likely to play a role in synaptic plasticity [256].…”

Section: Endocytosis and Kiss-and-runmentioning

confidence: 99%

Targeting Exocytosis: Ins and Outs of the Modulation of Quantal Dopamine Release

Westerink

2006

CNSNDDT

View full text Add to dashboard Cite

Dopaminergic neurotransmission is mediated by the vesicular release of dopamine (DA), i.e. DA exocytosis. DA exocytosis and its modulation are generally believed to affect neuronal communication, development, maintenance and survival, and contribute to extracellular DA levels in the brain. As a result, DA exocytosis likely plays an important role in several neurological and psychiatric disorders, like Parkinson's disease (PD) and schizophrenia. As exocytosis is part of a sophisticated ensemble of processes, it can be modulated at different levels, including DA synthesis, uptake and vesicular transport as well as Ca 2+ -homeostasis and exocytotic proteins. Nonetheless, to be effective, modulation of exocytosis should result in functional changes, which are reflected by changes in release frequency, vesicle contents, and the time course of the exocytotic event. As will be shown in this review, functional changes in DA exocytosis can be produced by e.g. pharmacological/drug treatment, feedback mechanisms and up/down-regulation of exocytosis-related proteins. Moreover, the mode of DA exocytosis, i.e. classical full fusion or kiss-and-run exocytosis, could also serve as a potential target for functional modulation of dopaminergic neurotransmission. Since the onset and progression of neurological and psychiatric disorders often show a strong correlation with changes in brain DA levels, DA synthesis, transport or uptake, the findings described in this review highlight the importance of the modulation of (the mode of) DA exocytosis for normal progression of dopaminergic neurotransmission and the potential of exocytotic processes as drug targets.

show abstract

Recycling of intact dense core vesicles in neurites of NGF‐treated PC12 cells

Cited by 15 publications

References 36 publications

Rapid recovery of releasable vesicles and formation of nonreleasable endosomes follow intense exocytosis in chromaffin cells

Rapid recovery of releasable vesicles and formation of nonreleasable endosomes follow intense exocytosis in chromaffin cells

Transient fusion ensures granule replenishment to enable repeated release after IgE-mediated mast cell degranulation

Targeting Exocytosis: Ins and Outs of the Modulation of Quantal Dopamine Release

Contact Info

Product

Resources

About