Recycling of &lt;I&gt;Calotropis gigantea&lt;/I&gt; Waste Fiber by Enzymatic Hydrolysis to Produce Bioethanol

Moeini, Seyed Sepehr; Dadashian, Fatemeh; Vahabzadeh, Farzaneh

doi:10.17485/ijst/2016/v9i9/79770

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…Waste from tea production amounts to billions of tons practically everywhere in the world, costing money and having negative effects on the environment for disposal [ 9 ]. These wastes are burned or disposed of in landfills.…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilization of β-galactosidase using a novel carrier alginate/tea waste: statistical optimization of beads modification and reusability

Abdella,

Hassan

2024

Bioprocess Biosyst Eng

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Abstractβ-galactosidase has been immobilized onto novel alginate/tea waste gel beads (Alg/TW) via covalent binding. Alg/TW beads were subjected to chemical modification through amination with polyethyleneimine (PEI) followed by activation with glutaraldehyde (GA). Chemical modification parameters including PEI concentration, PEI pH, and GA concentration were statistically optimized using Response Surface methodology (RSM) based on Box–Behnken Design (BBD). Analysis of variance (ANOVA) results confirmed the great significance of the model that had F value of 37.26 and P value < 0.05. Furthermore, the R2 value (0.9882), Adjusted R2 value (0.9617), and predicted R2 value (0.8130) referred to the high correlation between predicted and experimental values, demonstrating the fitness of the model. In addition, the coefficient of variation (CV) value was 2.90 that pointed to the accuracy of the experiments. The highest immobilization yield (IY) of β-galactosidase (75.1%) was given under optimized conditions of PEI concentration (4%), PEI pH (9.5), and GA concentration (2.5%). Alg/TW beads were characterized by FT-IR, TGA, and SEM techniques at each step of immobilization process. Moreover, the immobilized β-galactosidase revealed a very good reusability as it could be reused for 15 and 20 consecutive cycles keeping 99.7 and 72.1% of its initial activity, respectively. In conclusion, the environmental waste (tea waste) can be used in modern technological industries such as the food and pharmaceutical industry.

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Section: Introductionmentioning

confidence: 99%

Covalent immobilization of β-galactosidase using a novel carrier alginate/tea waste: statistical optimization of beads modification and reusability

Abdella,

Hassan

2024

Bioprocess Biosyst Eng

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“…Billions tons of tea wastes are produced in nearly all parts of the world. their disposal is costly and not devoid of environmental impact [1]. these wastes dumped into landfills or burned.…”

Section: Introductionmentioning

confidence: 99%

Tea wastes as an Alternative Sustainable Raw Material for Ethanol Production

2020

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I NtegRAtINg sustainability in life became an urgent necessity. Currently, tea sectors play important role in adopting new technologies in order to reduce tea wastes. Collection of these wastes for production of ethanol will contribute to the improvement of life and the environment. Simple technique was proposed for saccharification of spent tea leaves either by cellulase or fermentation with baker's yeast. Scanning electron microscope analysis showed certain modification for the morphology of both green and black tea leaves. Brunauer Emmett Teller analysis confirmed the increment in the surface area of both tea leaves. X-Ray Diffraction analysis proved the amorphous nature of their cellulose with little crystallinity. this nature eases their saccharification because cellulose is usually susceptible to degradation in its amorphous form. the percentage of produced ethanol was 74.4% from spent green tea leaves and 80.7% from spent black tea leaves after 24 h at mild temperature. the habit of drinking tea in egypt shed the light on a promising approach relies on the production of ethanol from tea wastes especially spent tea leaves.

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Latex-bearing plant (Calotropis procera) as a biorefinery for bioethanol production

Mahmoud

El‐Bery

Ali

et al. 2021

Biomass Conv. Bioref.

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Recycling of <I>Calotropis gigantea</I> Waste Fiber by Enzymatic Hydrolysis to Produce Bioethanol

Cited by 5 publications

References 14 publications

Covalent immobilization of β-galactosidase using a novel carrier alginate/tea waste: statistical optimization of beads modification and reusability

Covalent immobilization of β-galactosidase using a novel carrier alginate/tea waste: statistical optimization of beads modification and reusability

Tea wastes as an Alternative Sustainable Raw Material for Ethanol Production

Latex-bearing plant (Calotropis procera) as a biorefinery for bioethanol production

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