Recycling of superfine resolution agarose gel

Seng, T.-Y.; Singh, Rajinder; Faridah, Q.Z.; Tan, Soon Guan; Alwee, S.S.R.S.

doi:10.4238/2013.march.11.1

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…For example, high-resolution agarose can usually be reused for several time by the simple remelting process in the application of electrophoretic separation which is the routine method for resolving DNA. 39 To demonstrate the recycling of the agarose stamp, the used agarose stamp was soaked in the aqueous methanol solution for 24 h to elute most of the etchant and reaction products. Subsequently, the agarose stamp was placed in the boiling DI water with occasional swirling for the remelting process owing to the melting temperature of 90À95 °C of the agarose as shown in Figure 8c (see the video in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se₂ Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

et al. 2015

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A reactive mold-assisted chemical etching (MACE) process through an easy-to-make agarose stamp soaked in bromine methanol etchant to rapidly imprint larger area micro- and nanoarrays on CIGS substrates was demonstrated. Interestingly, by using the agarose stamp during the MACE process with and without additive containing oil and triton, CIGS microdome and microhole arrays can be formed on the CIGS substrate. Detailed formation mechanisms of microstructures and the chemical composition variation after the etching process were investigated. In addition, various microand nanostructures were also demonstrated by this universal approach. The microstructure arrays integrated into standard CIGS solar cells with thinner thickness can still achieve an efficiency of 11.22%, yielding an enhanced efficiency of ∼18% compared with that of their planar counterpart due to an excellent absorption behavior confirmed by the simulation results, which opens up a promising way for the realization of high-efficiency micro- or nanostructured thin-film solar cells. Finally, the complete dissolution of agarose stamp into hot water demonstrates an environmentally friendly method by the mold-assisted chemical etching process through an easy-to-make agarose stamp.

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Section: Resultsmentioning

confidence: 99%

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se₂ Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

et al. 2015

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“…Recycling of agarose after electrophoresis is very effective for cost-saving. Several reports are published [13,14], in which used agarose gels are simply boiled and poured to a gel tray. After cooling to make the recycle gel solid, the recycled gel is enough for applying another electrophoresis.…”

Section: Recycling and Reusing Of Agarosementioning

confidence: 99%

Cost-Effective Technical Tips for Agarose Gel Electrophoresis of Deoxyribonucleic Acid

Sasagawa¹

2021

Analytical Chemistry - Advancement, Perspectives and Applications

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Agarose gel electrophoresis is one of the most fundamental experiment in biochemistry and/or molecular biology, especially in analyzing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Many laboratories do agarose gel electrophoresis almost every day. Besides, sometimes we need to prepare tens of agarose gels at a time for training and/or practices of students. In such situations, the more costeffective way we have, the much more experiments in laboratories/trainings of students we can achieve. Actually, experiments of using agarose can be achieved in a more inexpensive way. In this manuscript, conditions of agarose gel electrophoresis experiment (agarose, buffer, and equipment) are considered, and achievements of such efforts are described.

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“…The generated SSR markers were separated using Superfine Resolution Agarose 3% (Seng et al 2013) using TAE 0.5x buffer (Brody & Kern…”

Section: Microsatellite Analysismentioning

confidence: 99%

HIGH OUTCROSSING RATE AND POLLEN DISPERSAL DISTANCE of Diospyros celebica Bakh. (EBENACEAE), AN ENDEMIC TREE SPECIES IN SULAWESI ISLAND, INDONESIA

Restu¹,

Gusmiaty²,

Larekeng

2017

BIOTROPIA

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Diosphyros celebicaBakh., also known as Sulawesi ebony, is an endemic to central and northern Sulawesi. Information about pollen dispersal patterns of D. celebica have not been previously investigated. This study was aimed to determine pollination type, percentage of selfing and outcrossing, as well as distance of pollen dispersal of D. celebica. This study was conducted at experimental forest of Universitas Hasanuddin, Maros District, South Sulawesi Province, Indonesia. One hundred and sixty six individuals of D. celebica consisted of adult trees and seedlings were analyzed in this study. Ninety four adult trees were selected to become parent trees. The trees were mapped with GPS coordinates. All samples were genotyped using four SSR markers loci. Parental analysis and determination of pollen dispersal patterns were carried out using Cervus 3.0.3. Results indicated that the evaluated markers were effective for assigning candidate of male parents to all evaluated seedlings. Donated pollens could come from male parents in any directions relative to female parent positions. Pollen dispersal pattern showed outcrossing pollination among different male parents (pollen donated trees). The results indicated that seeds were produced predominatly by outcrossing. Pollen dispersal reached up to 166 m. Pollen related processes were linked through female parents, pollinators availability and ecological environment. Simultaneous use of progeny genotyping, spatially explicit analysis of environmental variables and outcomes of plant-animal interactions were the key elements for an expanded approach to gene flow analysis considering dispersal via pollen and seeds. Research on pollen dispersal of D. celebica should be carried out in other forest types, such as mixed forest and highland forest.

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Recycling of superfine resolution agarose gel

Cited by 8 publications

References 11 publications

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se₂ Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se₂ Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

Cost-Effective Technical Tips for Agarose Gel Electrophoresis of Deoxyribonucleic Acid

HIGH OUTCROSSING RATE AND POLLEN DISPERSAL DISTANCE of Diospyros celebica Bakh. (EBENACEAE), AN ENDEMIC TREE SPECIES IN SULAWESI ISLAND, INDONESIA

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Recycling of superfine resolution agarose gel

Cited by 8 publications

References 11 publications

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se2 Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se2 Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

Cost-Effective Technical Tips for Agarose Gel Electrophoresis of Deoxyribonucleic Acid

HIGH OUTCROSSING RATE AND POLLEN DISPERSAL DISTANCE of Diospyros celebica Bakh. (EBENACEAE), AN ENDEMIC TREE SPECIES IN SULAWESI ISLAND, INDONESIA

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Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se₂ Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process

Large-Scale Micro- and Nanopatterns of Cu(In,Ga)Se₂ Thin Film Solar Cells by Mold-Assisted Chemical-Etching Process