RED AND FAR-RED INSENSITIVE 2, a RING-Domain Zinc Finger Protein, Mediates Phytochrome-Controlled Seedling Deetiolation Responses

Chen, Mingjie; Ni, Min

doi:10.1104/pp.105.073163

Cited by 22 publications

(17 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on these interaction screens 5 RING E3 ligases were selected to the study, represented by the following mutant lines; N656705 (AT5G65683), N686069 (AT1G61620), N372291 (AT3G29270), N2037522 and N67002 (AT4G17680), and N742646 (AT2G44410). In addition, a mutant line for COP1, cop1-6 , and RED AND FAR-RED INSENSITIVE 2 (RFI2) for which a role in mediating red and far-red light signaling and ubiquitination activity has been shown in vitro , were included (Stone et al, 2005; Chen and Ni, 2006a). This E3 ligase was selected as a candidate since its expression is regulated by circadian clock and rfi2-1 mutant flowers early (Chen and Ni, 2006b).…”

Section: Resultsmentioning

confidence: 99%

Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases in Arabidopsis

et al. 2017

View full text Add to dashboard Cite

Flowering time control integrates endogenous as well as environmental signals to promote flower development. The pathways and molecular networks involved are complex and integrate many modes of signal transduction. In plants ubiquitin mediated protein degradation pathway has been proposed to be as important mode of signaling as phosphorylation and transcription. To systematically study the role of ubiquitin signaling in the molecular regulation of flowering we have taken a genomic approach to identify flower related Ubiquitin Proteasome System components. As a large and versatile gene family the RING type ubiquitin E3 ligases were chosen as targets of the genomic screen. The complete list of Arabidopsis RING E3 ligases were retrieved and verified in the Arabidopsis genome v11 and their differential expression was used for their categorization into flower organs or developmental stages. Known regulators of flowering time or floral organ development were identified in these categories through literature search and representative mutants for each category were purchased for functional characterization by growth and morphological phenotyping. To this end, a workflow was developed for high throughput phenotypic screening of growth, morphology and flowering of nearly a thousand Arabidopsis plants in one experimental round.

show abstract

Section: Resultsmentioning

confidence: 99%

Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases in Arabidopsis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…By reverse genetics, we have identified candidate genes to regulators of shade-avoidance responses; ATHB2 orthologs (Ciarbelli et al 2008), photomorphogenesis and seedling establishment; FHY3/FAR1, NDPK2, PIF/PIL family, PP7, RAP1/ ATMYC2, RFI2 (Shen et al 2005;Yadav et al 2005;Chen and Ni 2006;Lin et al 2007;Genoud et al 2008;Leivar et al 2008), light-regulated proteolysis; COP1, EID1, LAF1, SPA1 (Yi and Deng 2005;Marrocco et al 2006;Yang et al 2009;Chen et al 2010;Fankhauser and Ulm 2011) and developmental transitions; CO, PFT1 (Suárez-López et al 2001;Cerdán and Chory 2003) (Table S2). Using the obtained sequence information in a reverse genetics approach, we have identified a novel petunia mutant carrying a mutation in CCT (CO, CO-like, TOC1) domain (Strayer et al 2000;Valverde 2011).…”

Section: Discussionmentioning

confidence: 99%

A reverse genetics approach identifies novel mutants in light responses and anthocyanin metabolism in petunia

Berenschot

Quecini

2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Flower color and plant architecture are important commercially valuable features for ornamental petunias (Petunia x hybrida Vilm.). Photoperception and light signaling are the major environmental factors controlling anthocyanin and chlorophyll biosynthesis and shadeavoidance responses in higher plants. The genetic regulators of these processes were investigated in petunia by in silico analyses and the sequence information was used to devise a reverse genetics approach to probe mutant populations. Petunia orthologs of photoreceptor, light-signaling components and anthocyanin metabolism genes were identified and investigated for functional conservation by phylogenetic and protein motif analyses. The expression profiles of photoreceptor gene families and of transcription factors regulating anthocyanin biosynthesis were obtained by bioinformatic tools. Two mutant populations, generated by an alkalyting agent and by gamma irradiation, were screened using a phenotype-independent, sequence-based method by high-throughput PCR-based assay. The strategy allowed the identification of novel mutant alleles for anthocyanin biosynthesis (CHALCONE SYNTHASE ) and regulation (PH4), and for light signaling (CONSTANS ) genes.

show abstract

“…In addition, the homologous PHYB transcript of N. attenuata was significantly down‐regulated in both ir‐ dcl3 and ir‐ dcl4 lines (Table S1). Light receptors entrain endogenous diurnal rhythms in plants and also control the plant's sensitivity to jasmonates during herbivore attack in Arabidopsis (Chen and Ni 2006). Interestingly, herbivores perform better on plants exposed to far‐red light, and total phenolics are lower in phyb mutants than in wild type plants (Moreno et al 2009).…”

Section: Discussionmentioning

confidence: 99%

DICER‐like Proteins and Their Role in Plant‐herbivore Interactions in Nicotiana attenuata

Bozorov¹,

Pandey

Dinh

et al. 2012

JIPB

View full text Add to dashboard Cite

DICER-like (DCL) proteins produce small RNAs that silence genes involved in development and defenses against viruses and pathogens. Which DCLs participate in plant-herbivore interactions remains unstudied. We identified and stably silenced four distinct DCL genes by RNAi in Nicotiana attenuata (Torrey ex. Watson), a model for the study of plant-herbivore interactions. Silencing DCL1 expression was lethal. Manduca sexta larvae performed significantly better on ir-dcl3 and ir-dcl4 plants, but not on ir-dcl2 plants compared to wild type plants. Phytohormones, defense metabolites and microarray analyses revealed that when DCL3 and DCL4 were silenced separately, herbivore resistance traits were regulated in distinctly different ways. Crossing of the lines revealed complex interactions in the patterns of regulation. Single ir-dcl4 and double ir-dcl2 ir-dcl3 plants were impaired in JA accumulation, while JA-Ile was increased in ir-dcl3 plants. Ir-dcl3 and ir-dcl4 plants were impaired in nicotine accumulation; silencing DCL2 in combination with either DCL3 or DCL4 restored nicotine levels to those of WT. Trypsin proteinase inhibitor activity and transcripts were only silenced in ir-dcl3 plants. We conclude that DCL2/3/4 interact in a complex manner to regulate anti-herbivore defenses and that these interactions significantly complicate the already challenging task of understanding smRNA function in the regulation of biotic interactions.

show abstract

RED AND FAR-RED INSENSITIVE 2, a RING-Domain Zinc Finger Protein, Mediates Phytochrome-Controlled Seedling Deetiolation Responses

Cited by 22 publications

References 64 publications

Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases in Arabidopsis

Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases in Arabidopsis

A reverse genetics approach identifies novel mutants in light responses and anthocyanin metabolism in petunia

DICER‐like Proteins and Their Role in Plant‐herbivore Interactions in Nicotiana attenuata

Contact Info

Product

Resources

About